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[184628_at]

Description F59E12.8 /REP_DB=WormBase Gene ID /WP=CE11532 /TR=O01898 /GB=AAB54258.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 03      4grp_total > 19      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 84
1 2 3 4 range
A 28.8
(-3)
A 		42.7
(-1.6)
A 		28.4
(-3)
A 		89.6
(3)
A 		61
B 22.3
(-0)
A 		15.7
(-3)
A 		20.9
(-0.7)
A 		29
(3)
A 		13
C 32.9
(-0.1)
A 		39.4
(0.6)
A 		6
(-3)
A 		62.2
(3)
A 		56
avr 28
(-1.6) 		32.6
(-1) 		18.4
(-3) 		60.3
(3) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 79
1 2 3 4 5 6 range
A 81.3
(3)
A 		42.2
(-0)
A 		52
(0.7)
A 		34.2
(-0.6)
A 		51.8
(0.7)
A 		3.8
(-3)
A 		78
B 5.6
(-3)
A 		39.4
(1)
A 		28.3
(-0.3)
A 		56.3
(3)
A 		33.1
(0.3)
A 		7.4
(-2.8)
A 		51
C 14.8
(-2)
A 		59.9
(1.4)
A 		9.3
(-2.5)
A 		80.7
(3)
A 		2.3
(-3)
A 		32.1
(-0.7)
A 		78
avr 33.9
(-0.3) 		47.2
(1.6) 		29.9
(-0.8) 		57.1
(3) 		29.1
(-0.9) 		14.4
(-3) 		43
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 10.5
(0.3)
A 		9.3
(-1)
A 		7.4
(-3)
A 		13.1
(3)
A 		6
B 7.1
(3)
A 		3.9
(-3)
A 		4.8
(-1.3)
A 		6.2
(1.3)
A 		3
C 10.9
(2.7)
A 		11.3
(3)
A 		2.1
(-3)
A 		10.5
(2.5)
A 		9
avr 9.5
(2.5) 		8.2
(1) 		4.8
(-3) 		9.9
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 15
(2.8)
A 		15
(2.8)
A 		11.3
(1.2)
A 		8.7
(0.2)
A 		15.6
(3)
A 		0.9
(-3)
A 		15
B 2
(-3)
A 		13.1
(2.2)
A 		6.9
(-0.7)
A 		14.9
(3)
A 		13.5
(2.3)
A 		2.8
(-2.6)
A 		13
C 5.5
(-1.4)
A 		18.3
(3)
A 		2.4
(-2.4)
A 		16.2
(2.3)
A 		0.7
(-3)
A 		10.7
(0.4)
A 		18
avr 7.5
(-1.5) 		15.5
(3) 		6.9
(-1.8) 		13.3
(1.8) 		9.9
(-0.1) 		4.8
(-3) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184628_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5460
Sequence Type Exemplar sequence
Representative Public ID F59E12.8 NCBI
Target Description F59E12.8 /REP_DB=WormBase Gene ID /WP=CE11532 /TR=O01898 /GB=AAB54258.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062697(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062697 NCBI Caenorhabditis elegans F59E12.8 (F59E12.8) mRNA, complete cds. 11/11 None
SNAP00000018063 ENSEMBL cdna:SNAP chromosome:CEL140:II:5634277:5636091:-1 9/11 None
F59E12.8 ENSEMBL cdna:known chromosome:CEL140:II:5634497:5636895:-1 gene:F59E12.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000018085 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5634494-5636893(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F59E12.8
Entrez Gene 186629 Entrez gene
SwissProt O01898 EMBL-EBI
WormBase CE11532 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB54258 Hypothetical protein F59E12.8 [Caenorhabditis elegans] ref|NP_495098.1| F59E12.8 [Caenorhabditis elegans] 0.0
blast CAE59152 Hypothetical protein CBG02457 [Caenorhabditis briggsae] 1.0E-109
blast AAB54258 Hypothetical protein F59E12.8 [Caenorhabditis elegans] ref|NP_495098.1| F59E12.8 [Caenorhabditis elegans] 3.0E-74
blast CAE59152 Hypothetical protein CBG02457 [Caenorhabditis briggsae] 3.0E-54
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495098.1 3 62-84,123-145,285-307
F59E12.8 3 62-84,123-145,285-307
SNAP00000018063 1 139-161
Sequence
Target Sequence 
>C. ELEGANS:184628_AT
aggaaactatcgtctgttcacaccatctgccgaccgtattccagaatgtccacatgagat
aacaacatccaaatgtaatgcaatattttctgaaattctatccaaacatcctccggaata
tggagatttaatgcaactcaacgatcgagaatttgtttcatcagtctgcccagccgctta
tattgtgcgcccaacgtttccatatcttgacgaattgaacgaaatgatcattaaaagaat
cgataatcattacacaccagcctatccagaattgacactaactggaaaaccgtcaaaatc
ttcgttctctctgaaccaactttcttcaatatttttgattcatcttctcggtgcattcgt
ttcaacattatttcttattgcggaacttgtgatcggtaaaacagattttctaactcatat
cgttgtgttttaccgtactcttaacttagactacggtaaaattttctttttcccaatcaa
tgtttcatcgtcttatggctcatact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGGAAACTATCGTCTGTTCACACCA 478 55 537 Antisense
GCCGACCGTATTCCAGAATGTCCAC 479 283 565 Antisense
AATTCTATCCAAACATCCTCCGGAA 433 177 630 Antisense
AGAATTTGTTTCATCAGTCTGCCCA 617 75 684 Antisense
CTGCCCAGCCGCTTATATTGTGCGC 462 237 702 Antisense
CGCCCAACGTTTCCATATCTTGACG 72 255 724 Antisense
AACCGTCAAAATCTTCGTTCTCTCT 377 145 824 Antisense
CGTTCTCTCTGAACCAACTTTCTTC 338 247 839 Antisense
TTTGATTCATCTTCTCGGTGCATTC 149 667 870 Antisense
TATCGTTGTGTTTTACCGTACTCTT 288 657 954 Antisense
GTTTCATCGTCTTATGGCTCATACT 16 451 1018 Antisense
Sequence Source Affymetrix Proprietary Database