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[184624_s_at]

Description C25H3.10 /REP_DB=WormBase Gene ID /WP=CE27090 /TR=Q18188 /GB=AAK31454.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 72      6grp_high1_10_10 > 37      4grp_highest_8_8 > 23      4grp_high_8_8 > 06      6grp_highest_nonorm > 40      6grp_total > 17      4grp_total > 02      6grp_highest_sub > Cu      6grp_total_sub > Decrease      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4488
1 2 3 4 range
A 655.7
(-2.8)
P 		758
(-2.7)
P 		538.5
(-3)
P 		4426.7
(3)
P 		3888
B 761.4
(-2.8)
P 		703.7
(-2.9)
P 		651.6
(-3)
P 		4106.5
(3)
P 		3455
C 753.9
(-2.9)
P 		813.2
(-2.8)
P 		654.8
(-3)
P 		5026.3
(3)
P 		4372
avr 723.7
(-2.8) 		758.3
(-2.8) 		615
(-3) 		4519.8
(3) 		3905

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 703
1 2 3 4 5 6 range
A 907.7
(1.7)
P 		700.8
(-0.3)
P 		1041.4
(3)
P 		590.3
(-1.3)
P 		418.8
(-3)
P 		636.2
(-0.9)
P 		623
B 1072.5
(3)
P 		689
(-0.9)
P 		626.4
(-1.5)
P 		518.8
(-2.6)
P 		475.3
(-3)
P 		647.3
(-1.3)
P 		597
C 1122.1
(3)
P 		784.3
(-0.4)
P 		571.2
(-2.5)
P 		519.2
(-3)
P 		544.1
(-2.8)
P 		709.7
(-1.1)
P 		603
avr 1034.1
(3) 		724.7
(-0.3) 		746.3
(-0.1) 		542.8
(-2.3) 		479.4
(-3) 		664.4
(-1) 		555
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 734
1 2 3 4 range
A 238.7
(-1.8)
P 		164.6
(-2.7)
P 		140.8
(-3)
P 		648
(3)
P 		507
B 242.8
(-2.2)
P 		173.8
(-2.8)
P 		151
(-3)
P 		875.1
(3)
P 		724
C 249.6
(-2.8)
P 		233.8
(-2.9)
P 		228.2
(-3)
P 		847.5
(3)
P 		619
avr 243.7
(-2.3) 		190.7
(-2.8) 		173.3
(-3) 		790.2
(3) 		617

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 312
1 2 3 4 5 6 range
A 167.3
(-1)
P 		249.2
(3)
P 		226.8
(1.9)
P 		149.7
(-1.9)
P 		126.4
(-3)
P 		154.9
(-1.6)
P 		123
B 373.2
(3)
P 		229.3
(-0.7)
P 		152.1
(-2.6)
P 		137.4
(-3)
P 		193.1
(-1.6)
P 		241.4
(-0.4)
P 		236
C 415.9
(3)
P 		239.3
(-0.4)
P 		145.4
(-2.2)
P 		104.4
(-3)
P 		176.1
(-1.6)
P 		237.6
(-0.4)
P 		312
avr 318.8
(3) 		239.3
(0.5) 		174.8
(-1.6) 		130.5
(-3) 		165.2
(-1.9) 		211.3
(-0.4) 		188
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184624_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4942
Sequence Type Exemplar sequence
Representative Public ID C25H3.10 NCBI
Target Description C25H3.10 /REP_DB=WormBase Gene ID /WP=CE27090 /TR=Q18188 /GB=AAK31454.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026811(9), NM_001026810(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026811 NCBI Caenorhabditis elegans C25H3.10b (C25H3.10) mRNA, complete cds. 9/11 None
NM_001026810 NCBI Caenorhabditis elegans C25H3.10a (C25H3.10) mRNA, complete cds. 11/11 None
SNAP00000016812 ENSEMBL cdna:SNAP chromosome:CEL140:II:5696661:5697587:-1 11/11 None
GENEFINDER00000016828 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5696661:5697665:-1 11/11 None
C25H3.10b ENSEMBL cdna:known chromosome:CEL140:II:5696634:5698924:-1 gene:C25H3.10 9/11 None
C25H3.10a ENSEMBL cdna:known chromosome:CEL140:II:5696661:5698881:-1 gene:C25H3.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5696657-5697662(-) 100.0 100.0
Public Domain and Genome References
Ensembl C25H3.10
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK31454 Hypothetical protein C25H3.10a [Caenorhabditis elegans] ref|NP_001021981.1| C25H3.10a [Caenorhabditis elegans] 1.0E-170
blast AAK31454 Hypothetical protein C25H3.10a [Caenorhabditis elegans] ref|NP_001021981.1| C25H3.10a [Caenorhabditis elegans] 1.0E-155
blast AAK31454 Hypothetical protein C25H3.10a [Caenorhabditis elegans] ref|NP_001021981.1| C25H3.10a [Caenorhabditis elegans] 1.0E-140
blast CAE59174 Hypothetical protein CBG02481 [Caenorhabditis briggsae] 1.0E-119
blast CAE59174 Hypothetical protein CBG02481 [Caenorhabditis briggsae] 1.0E-105
blast CAE59174 Hypothetical protein CBG02481 [Caenorhabditis briggsae] 2.0E-92
blast AAV28362 Hypothetical protein C25H3.10b [Caenorhabditis elegans] ref|NP_001021982.1| C25H3.10b [Caenorhabditis elegans] 9.0E-76
Protein Domains
Database ID Description E-Value
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 2.6E-4
Sequence
Target Sequence 
>C. ELEGANS:184624_S_AT
gtggatttactttggctagagatgaatggaagtgaagatgaacctattgaatttcttcaa
aaagttattcaaaaaacaaatttcactagaattgattgtttgaaattgggaagctcaaaa
gttgagtgtgacgtcacaaaattggaaaattgcatggcaagtttggtgtgtgcagaacta
attgttgactggagattcagagttcctgaggattttttcaatgaacttcatgttgagaag
ttaactcgattaacgattggaaatcaggaatctagaaacttctaccacccactttcggga
gatcacttcagaaagttcagaggagattggttggaaattgggcaaaatcgtctcagaagt
tcagatatatctaccttcctaaaaaattggaaattgggctccatttcttggattgaaatg
tacaacattgtcattagttttccaattcccgaattattgattttactgaaaaatgagagt
tgccagaaaacaccaacgacgtggaaagtgtctcgggatctagacgaaggaaaagaggaa
cttgaaattggattcttcaaaagtacaaacttctcgaaacagccatcattgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGGATTTACTTTGGCTAGAGATGA 438 489 193 Antisense
GCATGGCAAGTTTGGTGTGTGCAGA 311 311 344 Antisense
GTTGACTGGAGATTCAGAGTTCCTG 528 437 376 Antisense
GAATCTAGAAACTTCTACCACCCAC 193 327 460 Antisense
CTACCACCCACTTTCGGGAGATCAC 448 225 474 Antisense
TTGGGCAAAATCGTCTCAGAAGTTC 225 707 530 Antisense
AGTTCAGATATATCTACCTTCCTAA 62 63 550 Antisense
TGGAAATTGGGCTCCATTTCTTGGA 133 531 580 Antisense
TTGTCATTAGTTTTCCAATTCCCGA 371 707 620 Antisense
ACGTGGAAAGTGTCTCGGGATCTAG 463 95 691 Antisense
AACTTCTCGAAACAGCCATCATTGG 172 137 760 Antisense
Sequence Source Affymetrix Proprietary Database