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[184616_at]

Description Y18D10A.4 /REP_DB=WormBase Gene ID /WP=CE21400 /TR=Q9XW23 /GB=CAA22310.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 59      4grp_total > 09      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 119
1 2 3 4 range
A 27.6
(-1.9)
A 		5.4
(-3)
A 		38.5
(-1.3)
A 		124
(3)
A 		119
B 26.1
(0)
A 		35.1
(3)
A 		34.1
(2.7)
A 		17.1
(-3)
A 		18
C 22.7
(-3)
A 		49.7
(-0.8)
A 		26.3
(-2.7)
A 		94.9
(3)
A 		72
avr 25.5
(-3) 		30.1
(-2.5) 		33
(-2.2) 		78.7
(3) 		53

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 36
1 2 3 4 5 6 range
A 39.9
(3)
A 		19.9
(-0.3)
A 		20.5
(-0.2)
A 		3.6
(-3)
A 		11.8
(-1.6)
A 		27.9
(1)
A 		36
B 7.3
(-2.8)
A 		18.2
(-0.2)
A 		6.3
(-3)
A 		20.1
(0.2)
A 		24.5
(1.2)
A 		32.1
(3)
A 		26
C 22.5
(1)
A 		6.1
(-3)
A 		22.1
(0.9)
A 		30.9
(3)
A 		17.6
(-0.2)
A 		17.6
(-0.2)
A 		25
avr 23.2
(1.6) 		14.7
(-3) 		16.3
(-2.1) 		18.2
(-1.1) 		18
(-1.2) 		25.9
(3) 		11
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 10
(0.1)
A 		1.2
(-3)
A 		10.1
(0.1)
A 		18.2
(3)
A 		17
B 8.3
(2.5)
A 		8.7
(3)
A 		7.9
(2.1)
A 		3.6
(-3)
A 		5
C 7.5
(-3)
A 		14.3
(1.8)
A 		9.2
(-1.8)
A 		16
(3)
A 		9
avr 8.6
(-2.3) 		8.1
(-3) 		9.1
(-1.7) 		12.6
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 7.4
(3)
A 		7.1
(2.7)
A 		4.5
(0.3)
A 		0.9
(-3)
A 		3.6
(-0.5)
A 		6.8
(2.4)
A 		7
B 2.5
(-2.4)
A 		6.1
(-0.4)
A 		1.5
(-3)
A 		5.3
(-0.8)
A 		10
(1.9)
A 		12
(3)
A 		11
C 8.3
(3)
A 		1.9
(-3)
A 		5.6
(0.5)
A 		6.2
(1)
A 		5.7
(0.6)
A 		5.9
(0.8)
A 		6
avr 6.1
(0.1) 		5
(-1.5) 		3.9
(-3) 		4.1
(-2.7) 		6.4
(0.5) 		8.2
(3) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184616_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2109
Sequence Type Exemplar sequence
Representative Public ID Y18D10A.4 NCBI
Target Description Y18D10A.4 /REP_DB=WormBase Gene ID /WP=CE21400 /TR=Q9XW23 /GB=CAA22310.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060841(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060841 NCBI Caenorhabditis elegans Y18D10A.4 (Y18D10A.4) mRNA, complete cds. 11/11 None
SNAP00000000411 ENSEMBL cdna:SNAP chromosome:CEL140:I:12824025:12832786:1 9/11 None
GENEFINDER00000000465 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:12824025:12824549:1 11/11 None
Y18D10A.4 ENSEMBL cdna:known chromosome:CEL140:I:12824025:12824549:1 gene:Y18D10A.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:12823955-12824480(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y18D10A.4
Entrez Gene 189473 Entrez gene
SwissProt Q9XW23 EMBL-EBI
WormBase CE21400 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA22311 Hypothetical protein Y18D10A.5 [Caenorhabditis elegans] ref|NP_493243.1| Glycogen Synthase Kinase family member (gsk-3) [Caenorhabditis elegans] 0.0
blast AAD45354 GSK-3 [Caenorhabditis elegans] 0.0
blast CAE63499 Hypothetical protein CBG07972 [Caenorhabditis briggsae] 0.0
blast CAA22310 Hypothetical protein Y18D10A.4 [Caenorhabditis elegans] ref|NP_493242.1| Y18D10A.4 [Caenorhabditis elegans] 1.0E-51
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 8.0E-73
hanks 3.1.7 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; PCTAIRE1 2.0E-69
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 2.3E-91
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493242.1 3 54-76,86-108,135-157
Y18D10A.4 3 54-76,86-108,135-157
SNAP00000000411 3 54-76,86-108,129-151
GENEFINDER00000000465 3 54-76,86-108,135-157
Sequence
Target Sequence 
>C. ELEGANS:184616_AT
tgtcagagagatgtggggggccgccttgtctgcgtctcgtagacgggcggactctgagtg
tctgcgtctctgatgactcatcacacattgatggatctatcggttaccatttcttctcct
tagattgcctctcactctctctcactctctccactgctgctgttgtggctacagctacgt
cgtcgtcgtcatcgccgaaggcctttggggtgtcggtttctctgtttctctggagccttt
tggttgcttggtatgtggttggcgtgttgctagttactggaggaatggacagagagaaaa
tatatgtatggcagttctttcacagattgccaacgaagcggaaaattgagaattcctttg
ctttttcttcgttctttttttgcttcctgaagcct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTCAGAGAGATGTGGGGGGCCGCC 527 557 62 Antisense
CCTTGTCTGCGTCTCGTAGACGGGC 549 265 85 Antisense
GTAGACGGGCGGACTCTGAGTGTCT 161 457 100 Antisense
TGTCTGCGTCTCTGATGACTCATCA 268 557 120 Antisense
GATCTATCGGTTACCATTTCTTCTC 29 421 155 Antisense
CTGTTGTGGCTACAGCTACGTCGTC 382 237 221 Antisense
CATCGCCGAAGGCCTTTGGGGTGTC 104 211 251 Antisense
TTCTCTGGAGCCTTTTGGTTGCTTG 66 693 287 Antisense
GTTGGCGTGTTGCTAGTTACTGGAG 629 435 319 Antisense
GAGAATTCCTTTGCTTTTTCTTCGT 590 389 409 Antisense
GTTCTTTTTTTGCTTCCTGAAGCCT 511 441 432 Antisense
Sequence Source Affymetrix Proprietary Database