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[184615_at]

Description R06A10.1 /REP_DB=WormBase Gene ID /WP=CE12512 /TR=O44746 /GB=AAB96732.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 02      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 588
1 2 3 4 range
A 24.8
(-2.8)
A 		7.6
(-3)
A 		23.3
(-2.8)
A 		530.2
(3)
P 		523
B 5.2
(-3)
A 		46.4
(-2.4)
A 		24.2
(-2.7)
A 		447.1
(3)
P 		442
C 29.1
(-3)
A 		72.5
(-2.5)
A 		34.3
(-2.9)
A 		593.6
(3)
P 		565
avr 19.7
(-3) 		42.2
(-2.7) 		27.3
(-2.9) 		523.6
(3) 		504

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 382
1 2 3 4 5 6 range
A 4.7
(-3)
A 		76.4
(-1.5)
A 		64.9
(-1.8)
A 		102.8
(-1)
M 		203.7
(1.1)
P 		298.5
(3)
P 		294
B 8.6
(-3)
A 		72.1
(-1.8)
A 		66
(-1.9)
A 		79.2
(-1.7)
A 		334
(3)
P 		305.3
(2.5)
P 		325
C 110
(-1.5)
A 		47.5
(-2.5)
A 		43.3
(-2.6)
A 		20
(-3)
A 		386.2
(3)
P 		338.3
(2.2)
P 		366
avr 41.1
(-3) 		65.3
(-2.5) 		58.1
(-2.6) 		67.3
(-2.4) 		308
(2.9) 		314
(3) 		273
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 99
1 2 3 4 range
A 9
(-2.4)
A 		1.6
(-3)
A 		6.1
(-2.6)
A 		77.6
(3)
P 		76
B 1.6
(-3)
A 		11.5
(-2.4)
A 		5.6
(-2.7)
A 		95.3
(3)
P 		94
C 9.6
(-3)
A 		20.8
(-2.3)
A 		12
(-2.8)
A 		100.1
(3)
P 		91
avr 6.7
(-3) 		11.3
(-2.7) 		7.9
(-2.9) 		91
(3) 		84

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 135
1 2 3 4 5 6 range
A 0.9
(-3)
A 		27.2
(-0.8)
A 		14.1
(-1.9)
A 		26.1
(-0.9)
M 		61.5
(2.1)
P 		72.7
(3)
P 		72
B 3
(-3)
A 		24
(-2.1)
A 		16
(-2.4)
A 		21
(-2.2)
A 		135.7
(3)
P 		113.8
(2)
P 		133
C 40.8
(-1.2)
A 		14.5
(-2.5)
A 		11
(-2.7)
A 		4
(-3)
A 		125
(3)
P 		113.3
(2.4)
P 		121
avr 14.9
(-2.9) 		21.9
(-2.5) 		13.7
(-3) 		17
(-2.8) 		107.4
(3) 		99.9
(2.5) 		94
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184615_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.35
Sequence Type Exemplar sequence
Representative Public ID R06A10.1 NCBI
Target Description R06A10.1 /REP_DB=WormBase Gene ID /WP=CE12512 /TR=O44746 /GB=AAB96732.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058418(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058418 NCBI Caenorhabditis elegans R06A10.1 (R06A10.1) mRNA, complete cds. 11/11 None
GENEFINDER00000037730 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:1068989:1089696:1 11/11 None
R06A10.1 ENSEMBL cdna:known chromosome:CEL140:I:1085970:1088453:1 gene:R06A10.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:1087251-1088453(+) 80.17 80.17
Public Domain and Genome References
Gene Title
Ensembl R06A10.1
Entrez Gene 187633 Entrez gene
SwissProt O44746 EMBL-EBI
WormBase CE36707 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB96733 G protein, subunit alpha protein 1 [Caenorhabditis elegans] ref|NP_490817.3| G protein, Subunit Alpha family member (gsa-1) [Caenorhabditis elegans] gb|AAG32094.1| heterotrimeric G protein alpha subunit [Caenorhabditis elegans] dbj|BAA22402.1| G protein alpha subunit [Caenorhabditis elegans] dbj|BAA22401.1| G protein a(s) subunit [Caenorhabditis elegans] 1.0E-139
blast AAW02912 gsa-1 [Caenorhabditis briggsae] 1.0E-130
blast CAE74357 Hypothetical protein CBG22078 [Caenorhabditis briggsae] 1.0E-129
blast AAB96732 Hypothetical protein R06A10.1 [Caenorhabditis elegans] ref|NP_490819.2| R06A10.1 [Caenorhabditis elegans] 2.0E-68
blast CAE74362 Hypothetical protein CBG22086 [Caenorhabditis briggsae] 3.0E-48
Protein Domains
Database ID Description E-Value
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 1.7E-37
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 2.3E-107
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_490819.2 4 5-27,42-64,71-93,128-150
R06A10.1 4 5-27,42-64,71-93,128-150
GENEFINDER00000037730 7 132-154,448-470,480-502,509-531,566-588,609-631,658-680
Sequence
Target Sequence 
>C. ELEGANS:184615_AT
ctgttgcccgattcatttgcgcaataattgtagtgattgcctggttgtccgattaccacg
gaaatccgaccgaagatacgccaaaatcgaaatccgagcaggaaaatgtgacagaagacc
cgtatgagtggatttctcatcgaaaagcaattttgatcgcattcgccggaatttacatca
agatttttatctcgcaattgtttattctca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTGTTGCCCGATTCATTTGCGCAAT 456 239 80 Antisense
AGTGATTGCCTGGTTGTCCGATTAC 525 59 111 Antisense
TGTCCGATTACCACGGAAATCCGAC 630 555 125 Antisense
GGAAATCCGACCGAAGATACGCCAA 455 525 139 Antisense
GATACGCCAAAATCGAAATCCGAGC 381 423 154 Antisense
GAAAATGTGACAGAAGACCCGTATG 495 353 181 Antisense
GAAGACCCGTATGAGTGGATTTCTC 618 337 193 Antisense
AAAGCAATTTTGATCGCATTCGCCG 7 125 223 Antisense
GATCGCATTCGCCGGAATTTACATC 601 417 234 Antisense
TTTACATCAAGATTTTTATCTCGCA 490 673 251 Antisense
TTTATCTCGCAATTGTTTATTCTCA 84 675 265 Antisense
Sequence Source Affymetrix Proprietary Database