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[184587_s_at]

Description Y66H1B.4 /REP_DB=WormBase Gene ID /WP=CE20348 /TR=Q9Y194 /GB=AAC69001.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 22      6grp_high1_10_10 > 02      4grp_highest_8_8 > 25      4grp_high_8_8 > 59      6grp_highest_nonorm > 31      6grp_total > 33      4grp_total > 60      6grp_highest_sub > De      6grp_cgc > 20      6grp_high1_sub > De      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1254
1 2 3 4 range
A 1257.9
(2.2)
P 		950.6
(0.6)
P 		1400.3
(3)
P 		272.1
(-3)
P 		1128
B 1306.5
(2.1)
P 		1262.9
(1.9)
P 		1499.4
(3)
P 		245.9
(-3)
P 		1254
C 1263.6
(2.3)
P 		1171
(1.8)
P 		1402.4
(3)
P 		251.5
(-3)
P 		1151
avr 1276
(2.2) 		1128.2
(1.4) 		1434
(3) 		256.5
(-3) 		1178

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 895
1 2 3 4 5 6 range
A 1125.9
(3)
P 		1069.1
(2.5)
P 		985.7
(1.7)
P 		825.8
(0.1)
P 		498.3
(-3)
P 		603.2
(-2)
P 		628
B 1262.8
(3)
P 		859.4
(0.3)
P 		1110.1
(2)
P 		720.1
(-0.7)
P 		641.6
(-1.2)
P 		371.7
(-3)
P 		891
C 1266.2
(3)
P 		867.5
(0)
P 		1068
(1.5)
P 		569.3
(-2.2)
P 		540.6
(-2.4)
P 		460.2
(-3)
P 		806
avr 1218.3
(3) 		932
(0.7) 		1054.6
(1.7) 		705.1
(-1.2) 		560.2
(-2.3) 		478.4
(-3) 		740
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 449
1 2 3 4 range
A 458
(3)
P 		206.4
(-0.6)
P 		366.1
(1.7)
P 		39.8
(-3)
P 		418
B 416.6
(3)
P 		311.9
(1.3)
P 		347.4
(1.9)
P 		52.4
(-3)
P 		364
C 418.3
(2.1)
P 		336.7
(1)
P 		488.8
(3)
P 		42.4
(-3)
P 		446
avr 431
(3) 		285
(0.7) 		400.8
(2.5) 		44.9
(-3) 		386

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 355
1 2 3 4 5 6 range
A 207.6
(-1.4)
P 		380.2
(3)
P 		214.7
(-1.3)
P 		209.4
(-1.4)
P 		150.4
(-2.9)
P 		146.9
(-3)
P 		233
B 439.4
(3)
P 		286.1
(-0.1)
P 		269.6
(-0.4)
P 		190.7
(-2)
P 		260.7
(-0.6)
P 		138.6
(-3)
P 		301
C 469.3
(3)
P 		264.7
(-0.5)
P 		271.8
(-0.3)
P 		114.5
(-3)
P 		174.9
(-2)
P 		154
(-2.3)
P 		355
avr 372.1
(3) 		310.3
(1.4) 		252
(-0.2) 		171.5
(-2.3) 		195.3
(-1.7) 		146.5
(-3) 		226
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184587_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10858
Sequence Type Exemplar sequence
Representative Public ID Y66H1B.4 NCBI
Target Description Y66H1B.4 /REP_DB=WormBase Gene ID /WP=CE20348 /TR=Q9Y194 /GB=AAC69001.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067512(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067512 NCBI Caenorhabditis elegans Sphingosine Phosphate Lyase family member (spl-1) (spl-1) mRNA, complete cds. 11/11 None
Y66H1B.4 ENSEMBL cdna:known chromosome:CEL140:IV:379900:383185:-1 gene:Y66H1B.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:380017-383179(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol spl-1
Ensembl Y66H1B.4
Entrez Gene 176857 Entrez gene
SwissProt Q9Y194 EMBL-EBI
WormBase CE20348 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:259598_AT pyridoxal-dependent decarboxylase family protein at
CANINE_2:CFA.21559.1.S1_AT similar to sphingosine-1-phosphate lyase 1 cfa
CANINE_2:CFAAFFX.21765.1.S1_AT similar to sphingosine-1-phosphate lyase 1 cfa
DROSOPHILA_2:1624818_S_AT Sphingosine-1-phosphate lyase dm
DROSGENOME1:141536_AT Sphingosine-1-phosphate lyase dm
CHICKEN:GGA.16071.1.S1_S_AT sphingosine-1-phosphate lyase 1 gga
HG-U133_PLUS_2:208381_S_AT sphingosine-1-phosphate lyase 1 hs
HG-U133A:208381_S_AT sphingosine-1-phosphate lyase 1 hs
HG-U133A_2:208381_S_AT sphingosine-1-phosphate lyase 1 hs
HG-U95AV2:35876_S_AT sphingosine-1-phosphate lyase 1 hs
HU35KSUBB:RC_T86156_AT sphingosine-1-phosphate lyase 1 hs
HU35KSUBB:RC_R39923_AT sphingosine-1-phosphate lyase 1 hs
U133_X3P:G10190652_3P_A_AT sphingosine-1-phosphate lyase 1 hs
U133_X3P:HS.186613.1.S3_3P_AT sphingosine-1-phosphate lyase 1 hs
U133_X3P:HS.186613.1.S2_3P_AT sphingosine-1-phosphate lyase 1 hs
HG-U133A_2:212321_AT sphingosine-1-phosphate lyase 1 hs
HG-FOCUS:212321_AT sphingosine-1-phosphate lyase 1 hs
HG-U133_PLUS_2:212321_AT sphingosine-1-phosphate lyase 1 hs
HG-U133A:212321_AT sphingosine-1-phosphate lyase 1 hs
HG-U133A_2:212322_AT sphingosine-1-phosphate lyase 1 hs
HG-U133_PLUS_2:212322_AT sphingosine-1-phosphate lyase 1 hs
HG-U133A:212322_AT sphingosine-1-phosphate lyase 1 hs
HG-U95AV2:35875_AT sphingosine-1-phosphate lyase 1 hs
HG-U95B:44046_AT sphingosine-1-phosphate lyase 1 hs
HG-U95C:63400_AT sphingosine-1-phosphate lyase 1 hs
HG-U95E:87266_AT Sphingosine-1-phosphate lyase 1 hs
HG-U95D:91823_AT Sphingosine-1-phosphate lyase 1 hs
MG-U74AV2:96126_AT sphingosine phosphate lyase 1 mm
MG-U74AV2:96127_AT sphingosine phosphate lyase 1 mm
MOE430A:1415892_AT sphingosine phosphate lyase 1 mm
MOUSE430A_2:1415892_AT sphingosine phosphate lyase 1 mm
MOUSE430_2:1415892_AT sphingosine phosphate lyase 1 mm
MOE430A:1415893_AT sphingosine phosphate lyase 1 mm
MOUSE430A_2:1415893_AT sphingosine phosphate lyase 1 mm
MOUSE430_2:1415893_AT sphingosine phosphate lyase 1 mm
MU19KSUBA:TC19685_AT Sphingosine phosphate lyase 1, mRNA (cDNA clone MGC:13853 IMAGE:4212293) mm
MU19KSUBC:TC40894_AT Sphingosine phosphate lyase 1, mRNA (cDNA clone MGC:13853 IMAGE:4212293) mm
RAE230B:1382843_AT sphingosine phosphate lyase 1 rn
RAT230_2:1382843_AT sphingosine phosphate lyase 1 rn
RAE230A:1372890_AT Sphingosine phosphate lyase 1 rn
RAT230_2:1372890_AT Sphingosine phosphate lyase 1 rn
RG-U34B:RC_AA964871_AT Sphingosine phosphate lyase 1 rn
YEAST_2:1777112_AT Dihydrosphingosine phosphate lyase, regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6520 amino acid metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16831 carboxy-lyase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC69001 Sphingosine phosphate lyase protein 1 [Caenorhabditis elegans] ref|NP_499913.1| Sphingosine Phosphate Lyase family member (spl-1) [Caenorhabditis elegans] gb|AAD44756.1| sphingosine-1-phosphate lyase [Caenorhabditis elegans] sp|Q9Y194|SGPL_CAEEL Sphingosine-1-phosphate lyase (SP-lyase) (SPL) (Sphingosine-1-phosphate aldolase) 0.0
blast CAE65555 Hypothetical protein CBG10544 [Caenorhabditis briggsae] 0.0
blast NP_775139 sphingosine phosphate lyase 1 [Rattus norvegicus] emb|CAD55407.1| sphingosine-1-phosphate lyase [Rattus norvegicus] sp|Q8CHN6|SGPL1_RAT Sphingosine-1-phosphate lyase 1 (SP-lyase) (SPL) (Sphingosine-1-phosphate aldolase) 1.0E-113
Protein Domains
Database ID Description E-Value
Pfam IPR002129 EMBL-EBI Pyridoxal-dependent decarboxylase 2.7E-4
Pfam IPR002129 EMBL-EBI Pyridoxal-dependent decarboxylase 2.9E-7
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499913.1 1 31-53
Y66H1B.4 1 31-53
Sequence
Target Sequence 
>C. ELEGANS:184587_S_AT
gttccaaggaacttcatcacttccagtatttctcggttgccgattggtgtggaggcatct
atgccaccccgactattgcaggatcccgagctggagccaacactgccgtcgcctgggcca
cacttttatccttcggtcgagacgaatatgttcgaagatgtgctcaaattgtgaagcata
cacgaatgctggccgagaaaattgagaaaatcaaatggatcaagccttatggaaaatcgg
atgtttcattggtggcgttctccggaaatggtgtgaatatctacgaagtttctgacaaaa
tgatgaagctcggatggaatttgaacactctgcagaatccagcggcaatccacatttgtt
tgacaatcaatcaagcgaacgaggaagttgtgaatgcgttcgccgtcgaccttgagaaga
tttgtgaagaactcgctgcaaaaggtgaacaaaaagctgacagtggaatggctgcgatgt
atggaatggctgcgcaagtaccaaaatcagtagtggacgaggttatcgctctgtacattg
acgcaact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTCCAAGGAACTTCATCACTTCCA 616 439 1094 Antisense
ATCACTTCCAGTATTTCTCGGTTGC 657 27 1109 Antisense
TCTCGGTTGCCGATTGGTGTGGAGG 423 611 1124 Antisense
GGTGTGGAGGCATCTATGCCACCCC 705 499 1139 Antisense
CTATTGCAGGATCCCGAGCTGGAGC 421 213 1166 Antisense
CACTTTTATCCTTCGGTCGAGACGA 400 197 1214 Antisense
TTGAACACTCTGCAGAATCCAGCGG 280 703 1414 Antisense
GAAGTTGTGAATGCGTTCGCCGTCG 25 337 1477 Antisense
TTCGCCGTCGACCTTGAGAAGATTT 254 695 1492 Antisense
AGTGGACGAGGTTATCGCTCTGTAC 170 59 1605 Antisense
TATCGCTCTGTACATTGACGCAACT 202 657 1617 Antisense
Sequence Source Affymetrix Proprietary Database