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[184560_s_at]

Description F16G10.9 /REP_DB=WormBase Gene ID /WP=CE19398 /TR=O76594 /GB=AAC26277.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 29      4grp_total > 40      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 52
1 2 3 4 range
A 11.5
(-3)
A 		35.4
(2.4)
A 		38.2
(3)
A 		32.1
(1.6)
A 		27
B 2.7
(-3)
A 		15.7
(-1.5)
A 		54.8
(3)
A 		29
(0)
A 		52
C 9
(-3)
A 		47.7
(2.1)
A 		54.3
(3)
A 		19.1
(-1.7)
A 		45
avr 7.7
(-3) 		32.9
(0.7) 		49.1
(3) 		26.7
(-0.2) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 44
1 2 3 4 5 6 range
A 25.5
(-0.1)
A 		7.4
(-3)
A 		27.2
(0.2)
A 		23.1
(-0.5)
A 		44.9
(3)
A 		18.7
(-1.2)
A 		38
B 25.2
(3)
A 		5.2
(-2)
A 		1.3
(-3)
A 		1.3
(-3)
A 		5
(-2.1)
A 		10.3
(-0.7)
A 		24
C 2.7
(-2.9)
A 		37.3
(3)
A 		10.8
(-1.5)
A 		23.6
(0.7)
A 		1.9
(-3)
A 		16
(-0.6)
A 		35
avr 17.8
(3) 		16.6
(1.5) 		13.1
(-3) 		16
(0.7) 		17.3
(2.4) 		15
(-0.6) 		5
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 4.2
(-3)
A 		7.7
(0.6)
A 		10
(3)
A 		4.7
(-2.5)
A 		6
B 0.9
(-3)
A 		3.9
(-1.5)
A 		12.7
(3)
A 		6.2
(-0.3)
A 		12
C 3
(-3)
A 		13.7
(1)
A 		18.9
(3)
A 		3.2
(-2.9)
A 		16
avr 2.7
(-3) 		8.4
(0.1) 		13.9
(3) 		4.7
(-1.9) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 4.7
(-1.9)
A 		2.6
(-3)
A 		5.9
(-1.2)
A 		5.8
(-1.3)
A 		13.6
(3)
A 		4.5
(-2)
A 		11
B 8.8
(3)
A 		1.7
(-2)
A 		0.3
(-3)
A 		0.3
(-3)
A 		2
(-1.8)
A 		3.8
(-0.5)
A 		9
C 1
(-2.8)
A 		11.4
(3)
A 		2.7
(-1.8)
A 		4.8
(-0.7)
A 		0.6
(-3)
A 		5.4
(-0.3)
A 		11
avr 4.8
(1.5) 		5.2
(2.5) 		3
(-3) 		3.6
(-1.5) 		5.4
(3) 		4.6
(1) 		2
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184560_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5910
Sequence Type Exemplar sequence
Representative Public ID F16G10.9 NCBI
Target Description F16G10.9 /REP_DB=WormBase Gene ID /WP=CE19398 /TR=O76594 /GB=AAC26277.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061862(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061862 NCBI Caenorhabditis elegans F16G10.9 (F16G10.9) mRNA, complete cds. 11/11 None
SNAP00000023119 ENSEMBL cdna:SNAP chromosome:CEL140:II:2384227:2385530:-1 9/11 None
F16G10.9 ENSEMBL cdna:known chromosome:CEL140:II:2384187:2386710:-1 gene:F16G10.9 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000023134 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2384225-2385626(-) 97.06 97.06
Public Domain and Genome References
Gene Title
Ensembl F16G10.9
Entrez Gene 173593 Entrez gene
SwissProt O76594 EMBL-EBI
WormBase CE19398 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC26277 Hypothetical protein F16G10.9 [Caenorhabditis elegans] ref|NP_494263.1| F16G10.9 [Caenorhabditis elegans] 1.0E-120
blast NP_508647 T14G12.6 [Caenorhabditis elegans] gb|AAA82438.1| Hypothetical protein T14G12.6 [Caenorhabditis elegans] 2.0E-84
blast AAC26277 Hypothetical protein F16G10.9 [Caenorhabditis elegans] ref|NP_494263.1| F16G10.9 [Caenorhabditis elegans] 4.0E-74
blast AAC26276 Hypothetical protein F16G10.8 [Caenorhabditis elegans] ref|NP_494262.1| F16G10.8 [Caenorhabditis elegans] 6.0E-71
blast NP_508647 T14G12.6 [Caenorhabditis elegans] gb|AAA82438.1| Hypothetical protein T14G12.6 [Caenorhabditis elegans] 1.0E-55
blast AAC26276 Hypothetical protein F16G10.8 [Caenorhabditis elegans] ref|NP_494262.1| F16G10.8 [Caenorhabditis elegans] 6.0E-48
Protein Domains
Database ID Description E-Value
Pfam IPR003326 EMBL-EBI Protein of unknown function DUF130 1.5E-78
Pfam IPR003326 EMBL-EBI Protein of unknown function DUF130 1.5E-78
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494263.1 1 12-34
F16G10.9 1 12-34
Sequence
Target Sequence 
>C. ELEGANS:184560_S_AT
tccgagcacattggcacctgtggaaagtactatggctcctggggtgagcacaatgactcc
tgggggtatttatacctcagcggaaaccgaggcgcctgaggtaatcctacaattgacaaa
tctctcaaaattccagctggtttcagaacaactatgcaaaattagtgcagccacgtgccc
agatttggcgtcagtgatctcaggtcaagtagccatgacaactgacacggacggatgcat
tgaattatcgtgtgtacctggaaatgatccatctcttactgcttcatttgataacagtga
aataccaccacctactccaggtgacgtaggtgatgatttttcgattagagtcccattacc
gcctcaagaagttgtcggtggattaagtggatattatggactcgtgtgtgagaataataa
gttgaaggcaacgaagtatccacttggaattgacacttataccggcagtggtatttttgg
agcggatgggtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCGAGCACATTGGCACCTGTGGAA 330 595 156 Antisense
GCACAATGACTCCTGGGGGTATTTA 260 317 203 Antisense
TGGGGGTATTTATACCTCAGCGGAA 191 551 216 Antisense
ATTTGGCGTCAGTGATCTCAGGTCA 245 19 338 Antisense
GTGATCTCAGGTCAAGTAGCCATGA 190 483 349 Antisense
GTAGCCATGACAACTGACACGGACG 407 457 364 Antisense
GACACGGACGGATGCATTGAATTAT 508 363 379 Antisense
GAAATGATCCATCTCTTACTGCTTC 456 361 416 Antisense
TCCCATTACCGCCTCAAGAAGTTGT 263 597 506 Antisense
TTGGAATTGACACTTATACCGGCAG 576 681 599 Antisense
GTGGTATTTTTGGAGCGGATGGGTC 507 487 623 Antisense
Sequence Source Affymetrix Proprietary Database