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[184558_at]

Description Y45F10A.5 /REP_DB=WormBase Gene ID /WP=CE18396 /TR=O62455 /GB=CAA16362.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 33      6grp_high1_10_10 > 23      4grp_highest_8_8 > 39      4grp_high_8_8 > 04      6grp_highest_nonorm > 13      6grp_total > 12      4grp_total > 12      6grp_highest_sub > 02      6grp_cgc > 62      6grp_high1_sub > 02      6grp_cgc_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4202
1 2 3 4 range
A 2976.7
(-0.3)
P 		1738.1
(-2.5)
P 		1422.4
(-3)
P 		4923.6
(3)
P 		3501
B 2380.4
(-1.1)
P 		1446.1
(-2.6)
P 		1208.2
(-3)
P 		4848.9
(3)
P 		3641
C 2325
(-1.4)
P 		1408.5
(-2.7)
P 		1224.6
(-3)
P 		5409.8
(3)
P 		4185
avr 2560.7
(-1) 		1530.9
(-2.6) 		1285.1
(-3) 		5060.8
(3) 		3776

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2227
1 2 3 4 5 6 range
A 2883.7
(1.3)
P 		2748.3
(0.9)
P 		3497.2
(3)
P 		2809.4
(1.1)
P 		1899.4
(-1.5)
P 		1348.2
(-3)
P 		2149
B 2929.9
(1)
P 		3527.7
(3)
P 		2172.4
(-1.5)
P 		2007.4
(-2)
P 		2163.3
(-1.5)
P 		1716.2
(-3)
P 		1812
C 2738.7
(0.7)
P 		3575.6
(3)
P 		2384
(-0.3)
P 		2277
(-0.6)
P 		2043.5
(-1.3)
P 		1424.8
(-3)
P 		2151
avr 2850.8
(1.5) 		3283.9
(3) 		2684.5
(1) 		2364.6
(-0.1) 		2035.4
(-1.2) 		1496.4
(-3) 		1788
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 804
1 2 3 4 range
A 1083.8
(3)
P 		377.3
(-3)
P 		371.8
(-3)
P 		720.7
(-0.1)
P 		712
B 759.1
(0.8)
P 		357.1
(-2.4)
P 		280
(-3)
P 		1033.3
(3)
P 		753
C 769.6
(1.3)
P 		405
(-3)
P 		426.8
(-2.7)
P 		912.2
(3)
P 		507
avr 870.8
(2.8) 		379.8
(-2.8) 		359.5
(-3) 		888.7
(3) 		529

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 846
1 2 3 4 5 6 range
A 531.6
(-1.1)
P 		977.4
(3)
P 		761.6
(1)
P 		712.5
(0.6)
P 		573.2
(-0.7)
P 		328.2
(-3)
P 		649
B 1019.4
(1.6)
P 		1174.2
(3)
P 		527.5
(-3)
P 		531.7
(-3)
P 		878.9
(0.3)
P 		640
(-2)
P 		647
C 1015.1
(2.3)
P 		1091
(3)
P 		606.8
(-1.6)
P 		457.9
(-3)
P 		661.2
(-1.1)
P 		477
(-2.8)
P 		633
avr 855.4
(0.7) 		1080.9
(3) 		632
(-1.5) 		567.4
(-2.1) 		704.4
(-0.8) 		481.7
(-3) 		599
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184558_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10238
Sequence Type Exemplar sequence
Representative Public ID Y45F10A.5 NCBI
Target Description Y45F10A.5 /REP_DB=WormBase Gene ID /WP=CE18396 /TR=O62455 /GB=CAA16362.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070202(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070202 NCBI Caenorhabditis elegans Neuropeptide-Like Protein family member (nlp-17) (nlp-17) mRNA, complete cds. 11/11 None
GENEFINDER00000021297 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:13497595:13505679:-1 9/11 None
Y45F10A.5 ENSEMBL cdna:known chromosome:CEL140:IV:13505236:13505730:-1 gene:Y45F10A.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:13505374-13505685(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nlp-17
Ensembl Y45F10A.5
Entrez Gene 178319 Entrez gene
SwissProt O62455 EMBL-EBI
WormBase CE18396 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA16368 Hypothetical protein Y45F10A.6a [Caenorhabditis elegans] emb|CAA16349.1| Hypothetical protein Y45F10A.6a [Caenorhabditis elegans] ref|NP_502598.1| Y45F10A.6a [Caenorhabditis elegans] 1.0E-162
blast CAC42378 Hypothetical protein Y45F10A.6b [Caenorhabditis elegans] emb|CAC42374.1| Hypothetical protein Y45F10A.6b [Caenorhabditis elegans] ref|NP_502599.1| Y45F10A.6b [Caenorhabditis elegans] 1.0E-162
blast CAE58594 Hypothetical protein CBG01761 [Caenorhabditis briggsae] 1.0E-149
blast CAA16362 Hypothetical protein Y45F10A.5 [Caenorhabditis elegans] ref|NP_502603.1| Neuropeptide-Like Protein family member (nlp-17) [Caenorhabditis elegans] 2.0E-33
blast CAE58593 Hypothetical protein CBG01760 [Caenorhabditis briggsae] 7.0E-25
Protein Domains
Database ID Description E-Value
Pfam IPR004182 EMBL-EBI GRAM domain 4.6E-27
Sequence
Target Sequence 
>C. ELEGANS:184558_AT
tcagcaagtcaattctcttctgccttttggttttggtattcaatgtttttggagctaact
ttgaaaatgatcaggatgtaatgcgtcccccattccaagcccttaaacgtggtagtttgt
caaacatgatgagaatcggaaagcgacaaatgtctcgacaacaggaatacgtacaatttc
caaatgaaggtgttgtgccgtgtgagagctgtaatttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCAGCAAGTCAATTCTCTTCTGCCT 59 625 17 Antisense
TCTCTTCTGCCTTTTGGTTTTGGTA 323 613 30 Antisense
TCTGCCTTTTGGTTTTGGTATTCAA 278 609 35 Antisense
GGTATTCAATGTTTTTGGAGCTAAC 210 505 51 Antisense
TGATCAGGATGTAATGCGTCCCCCA 557 565 84 Antisense
CATTCCAAGCCCTTAAACGTGGTAG 83 205 107 Antisense
GGAAAGCGACAAATGTCTCGACAAC 607 527 154 Antisense
GTCTCGACAACAGGAATACGTACAA 212 471 168 Antisense
GAATACGTACAATTTCCAAATGAAG 428 325 181 Antisense
GAAGGTGTTGTGCCGTGTGAGAGCT 347 335 202 Antisense
TGTGCCGTGTGAGAGCTGTAATTTG 42 555 210 Antisense
Sequence Source Affymetrix Proprietary Database