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[184555_at]

Description T20D4.12 /REP_DB=WormBase Gene ID /WP=CE13802 /TR=P91463 /GB=AAB37585.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 61      4grp_total > 40      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 103
1 2 3 4 range
A 17.4
(-2.7)
A 		12.9
(-3)
A 		99.5
(3)
P 		37.8
(-1.3)
A 		87
B 1.7
(-3)
A 		41.9
(-0.6)
A 		101.4
(3)
P 		49.7
(-0.1)
A 		100
C 1
(-3)
A 		17.3
(-2)
A 		103.9
(3)
P 		35.4
(-1)
A 		103
avr 6.7
(-3) 		24
(-1.9) 		101.6
(3) 		41
(-0.8) 		95

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 144
1 2 3 4 5 6 range
A 2.8
(-3)
A 		12.3
(-2.5)
A 		94.6
(1.9)
P 		116.3
(3)
P 		51.2
(-0.4)
P 		5.7
(-2.8)
A 		114
B 1.9
(-3)
A 		29
(-1.9)
A 		146.3
(3)
P 		136.4
(2.6)
P 		40.4
(-1.4)
P 		42.4
(-1.3)
A 		144
C 2.4
(-3)
A 		32.3
(-1)
A 		72
(1.6)
P 		92.5
(3)
P 		35.4
(-0.8)
P 		30.1
(-1.2)
P 		90
avr 2.4
(-3) 		24.5
(-1.8) 		104.3
(2.4) 		115.1
(3) 		42.3
(-0.9) 		26.1
(-1.7) 		113
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 36
1 2 3 4 range
A 6.3
(-2.1)
A 		2.8
(-3)
A 		26
(3)
P 		5.5
(-2.3)
A 		23
B 0.6
(-3)
A 		10.3
(-0.5)
A 		23.5
(3)
P 		10.6
(-0.4)
A 		23
C 0.3
(-3)
A 		5
(-2.2)
A 		36.2
(3)
P 		6
(-2)
A 		36
avr 2.4
(-3) 		6
(-2.2) 		28.6
(3) 		7.4
(-1.9) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 36
1 2 3 4 5 6 range
A 0.5
(-3)
A 		4.4
(-2.2)
A 		20.6
(1.2)
P 		29.5
(3)
P 		15.4
(0.1)
P 		1.4
(-2.8)
A 		29
B 0.7
(-3)
A 		9.7
(-1.5)
A 		35.5
(2.9)
P 		36.1
(3)
P 		16.4
(-0.3)
P 		15.8
(-0.4)
A 		35
C 0.9
(-3)
A 		9.9
(0.1)
A 		18.3
(2.9)
P 		18.6
(3)
P 		11.5
(0.6)
P 		10.1
(0.1)
P 		18
avr 0.7
(-3) 		8
(-1.4) 		24.8
(2.3) 		28.1
(3) 		14.4
(0) 		9.1
(-1.2) 		27
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184555_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15876
Sequence Type Exemplar sequence
Representative Public ID T20D4.12 NCBI
Target Description T20D4.12 /REP_DB=WormBase Gene ID /WP=CE13802 /TR=P91463 /GB=AAB37585.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071548(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071548 NCBI Caenorhabditis elegans T20D4.12 (T20D4.12) mRNA, complete cds. 11/11 None
SNAP00000008844 ENSEMBL cdna:SNAP chromosome:CEL140:V:3396901:3397597:1 11/11 None
GENEFINDER00000008865 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3396901:3397597:1 11/11 None
T20D4.12 ENSEMBL cdna:known chromosome:CEL140:V:3396000:3397597:1 gene:T20D4.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3395999-3397597(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T20D4.12
Entrez Gene 188648 Entrez gene
SwissProt P91463 EMBL-EBI
WormBase CE13802 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37585 Hypothetical protein T20D4.12 [Caenorhabditis elegans] ref|NP_503949.1| T20D4.12 [Caenorhabditis elegans] 1.0E-128
blast AAB37585 Hypothetical protein T20D4.12 [Caenorhabditis elegans] ref|NP_503949.1| T20D4.12 [Caenorhabditis elegans] 1.0E-116
blast AAB37587 Hypothetical protein T20D4.10 [Caenorhabditis elegans] ref|NP_503951.1| T20D4.10 [Caenorhabditis elegans] 4.0E-75
blast AAB37587 Hypothetical protein T20D4.10 [Caenorhabditis elegans] ref|NP_503951.1| T20D4.10 [Caenorhabditis elegans] 5.0E-75
blast AAC04403 Hypothetical protein C17B7.9 [Caenorhabditis elegans] ref|NP_503923.1| C17B7.9 [Caenorhabditis elegans] 3.0E-73
Protein Domains
Database ID Description E-Value
Pfam IPR002542 EMBL-EBI Protein of unknown function DUF19 1.5E-95
Pfam IPR002542 EMBL-EBI Protein of unknown function DUF19 1.5E-95
Sequence
Target Sequence 
>C. ELEGANS:184555_AT
gattcctcaaacttgttgtcattggcactgtgtttttagaagtggctaatgcagcatttg
cggaggaagaaatccttgtgtgtactgaagaagagtcacttaggtcacttttttgtgcca
tgcgtattcccccttttatcaacaaaactgaactactcgacatgaatgacaaaaaggagg
taaaagaattgaaggaggagtgccataacgtactcagttgtttcaacaaaatcaaatgtt
tgggcaaaaatggagatcaaataccgaagcttagggttgttggaaagtattgcaaagcat
tggactacgtacacgataattttgccgagtgtagtgacaaattgaatgcaaagaaatcaa
catgttttgatgactgggacccaattccaaacaaagttcatttcgaaaaagatccaataa
aaaaagagcagttaagaagtgaggcttgcaagatatattttggaaaagacgactgcatga
tgaaggaaattaaggcaacatgtggacaacaagaatgggacgccttcagagtgcatttca
ttaacatcgcaggagga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTCCTCAAACTTGTTGTCATTGG 478 433 83 Antisense
ACTTGTTGTCATTGGCACTGTGTTT 150 105 93 Antisense
GGAAGAAATCCTTGTGTGTACTGAA 116 531 147 Antisense
GAAGAGTCACTTAGGTCACTTTTTT 31 335 172 Antisense
ACTTTTTTGTGCCATGCGTATTCCC 317 103 189 Antisense
AACTGAACTACTCGACATGAATGAC 267 143 228 Antisense
GAAGGAGGAGTGCCATAACGTACTC 239 335 273 Antisense
GCAAAGCATTGGACTACGTACACGA 146 319 374 Antisense
CGATAATTTTGCCGAGTGTAGTGAC 686 223 396 Antisense
GACGCCTTCAGAGTGCATTTCATTA 687 379 601 Antisense
GCATTTCATTAACATCGCAGGAGGA 53 307 615 Antisense
Sequence Source Affymetrix Proprietary Database