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[184551_at]

Description H27M09.4 /REP_DB=WormBase Gene ID /WP=CE25036 /TR=Q9N5K5 /GB=AAF39908.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 58      4grp_total > 10      6grp_cgc > 28      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 272
1 2 3 4 range
A 20.1
(-2.7)
A 		77.3
(-0.6)
A 		12.6
(-3)
A 		174.7
(3)
P 		162
B 25.5
(-3)
A 		29.5
(-2.9)
A 		31.2
(-2.9)
A 		284.1
(3)
P 		259
C 16.7
(-3)
A 		51
(-2.2)
A 		73.5
(-1.6)
A 		268.8
(3)
P 		252
avr 20.8
(-3) 		52.6
(-2.1) 		39.1
(-2.5) 		242.5
(3) 		222

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1679
1 2 3 4 5 6 range
A 575.7
(-1.1)
P 		99.7
(-2.8)
P 		51.9
(-2.9)
A 		34.2
(-3)
A 		77.7
(-2.8)
P 		1713.2
(3)
P 		1679
B 766.5
(0.3)
P 		64.9
(-2.9)
P 		68.4
(-2.9)
A 		35.3
(-3)
A 		62.7
(-2.9)
P 		1376.7
(3)
P 		1341
C 982.6
(1.2)
P 		86.2
(-2.8)
P 		52.2
(-3)
A 		41.5
(-3)
A 		76.6
(-2.8)
P 		1400.8
(3)
P 		1359
avr 774.9
(0) 		83.6
(-2.8) 		57.5
(-2.9) 		37
(-3) 		72.3
(-2.9) 		1496.9
(3) 		1460
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 57
1 2 3 4 range
A 7.3
(-1.9)
A 		16.8
(0.6)
A 		3.3
(-3)
A 		25.6
(3)
P 		22
B 8.1
(-2.9)
A 		7.3
(-3)
A 		7.2
(-3)
A 		60.5
(3)
P 		53
C 5.5
(-3)
A 		14.7
(-1.6)
A 		25.6
(0)
A 		45.3
(3)
P 		40
avr 7
(-3) 		12.9
(-2) 		12
(-2.2) 		43.8
(3) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 505
1 2 3 4 5 6 range
A 106.1
(-1.6)
P 		35.4
(-2.6)
P 		11.3
(-3)
A 		8.7
(-3)
A 		23.5
(-2.8)
P 		417.1
(3)
P 		408
B 266.7
(0.1)
P 		21.6
(-2.9)
P 		16.6
(-2.9)
A 		9.3
(-3)
A 		25.5
(-2.8)
P 		513.4
(3)
P 		504
C 364.2
(1.6)
P 		26.3
(-2.8)
P 		13.3
(-2.9)
A 		8.3
(-3)
A 		24.8
(-2.8)
P 		468.9
(3)
P 		461
avr 245.7
(0.1) 		27.8
(-2.8) 		13.7
(-2.9) 		8.8
(-3) 		24.6
(-2.8) 		466.5
(3) 		458
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184551_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.597
Sequence Type Exemplar sequence
Representative Public ID H27M09.4 NCBI
Target Description H27M09.4 /REP_DB=WormBase Gene ID /WP=CE25036 /TR=Q9N5K5 /GB=AAF39908.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059557(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059557 NCBI Caenorhabditis elegans DumPY : shorter than wild-type family member (dpy-14) (dpy-14) mRNA, complete cds. 11/11 None
GENEFINDER00000019145 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6851624:6853224:1 11/11 None
SNAP00000019134 ENSEMBL cdna:SNAP chromosome:CEL140:I:6851755:6853224:1 11/11 None
H27M09.4.1 ENSEMBL cdna:known chromosome:CEL140:I:6851731:6853263:1 gene:H27M09.4 11/11 None
H27M09.4.2 ENSEMBL cdna:known chromosome:CEL140:I:6851731:6853321:1 gene:H27M09.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6851685-6853155(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol dpy-14
Ensembl H27M09.4
Entrez Gene 172410 Entrez gene
SwissProt Q9N5K5 EMBL-EBI
WormBase CE25036 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6817 phosphate transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5737 cytoplasm inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
42302 structural constituent of cuticle inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF39908 Dumpy : shorter than wild-type protein 14 [Caenorhabditis elegans] ref|NP_491958.1| DumPY : shorter than wild-type family member (dpy-14) [Caenorhabditis elegans] gb|AAP97871.1| COLlagen structural gene, probably a critical component of the embryonically derived outer layer of the L1 cuticle (31.8 kD) (col-59) [Caenorhabditis elegans] 1.0E-111
blast AAF39908 Dumpy : shorter than wild-type protein 14 [Caenorhabditis elegans] ref|NP_491958.1| DumPY : shorter than wild-type family member (dpy-14) [Caenorhabditis elegans] gb|AAP97871.1| COLlagen structural gene, probably a critical component of the embryonically derived outer layer of the L1 cuticle (31.8 kD) (col-59) [Caenorhabditis elegans] 1.0E-102
blast CAE67301 Hypothetical protein CBG12754 [Caenorhabditis briggsae] 1.0E-101
blast CAE67301 Hypothetical protein CBG12754 [Caenorhabditis briggsae] 1.0E-93
Protein Domains
Database ID Description E-Value
Pfam IPR002486 EMBL-EBI Nematode cuticle collagen, N-terminal 3.2E-7
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 8.6E-11
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 2.3E-4
Pfam IPR002486 EMBL-EBI Nematode cuticle collagen, N-terminal 6.9E-6
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 8.6E-11
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 2.3E-4
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491958.1 1 13-35
H27M09.4.1 1 13-35
H27M09.4.2 1 13-35
GENEFINDER00000019145 2 83-105,115-137
SNAP00000019134 1 13-35
Sequence
Target Sequence 
>C. ELEGANS:184551_AT
ggaccagttgttaatgctgaaccagaaccacaatgttgtacatgccaacaaggaaaggct
ggaccaccaggaccaccaggagatgatggaaaggacggaaatgatggatcagctggaaat
gatgctaagaatggaaaggatggaggtgttggaccatctgatggacttcaatctgaacca
tgcatgatctgcccaccaggtgcacaaggacttggaggtgctccaggagccaagggacca
caaggaccacgtggatcaccaggactttccggtgttgatggaagacgtggagagccaggt
atgtctggaccagctggaactcaaggagaaccaggaccacaaggaccaccaggaaagaag
ggagatgatggaagagttattaatgtcaatggaccaccaggagccggaggagccccagga
ccacaaggacgcaagggagaacgtggaccaaagggagttccaggatcagttcatcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGACCAGTTGTTAATGCTGAACCAG 248 523 358 Antisense
AGAACCACAATGTTGTACATGCCAA 705 73 381 Antisense
CAACAAGGAAAGGCTGGACCACCAG 124 189 403 Antisense
GGAGGTGTTGGACCATCTGATGGAC 252 517 499 Antisense
GACCATCTGATGGACTTCAATCTGA 537 383 509 Antisense
TCAATCTGAACCATGCATGATCTGC 422 633 525 Antisense
GCACAAGGACTTGGAGGTGCTCCAG 605 315 559 Antisense
GGATCACCAGGACTTTCCGGTGTTG 60 511 610 Antisense
GAGAGCCAGGTATGTCTGGACCAGC 595 393 647 Antisense
GACCAGCTGGAACTCAAGGAGAACC 322 383 665 Antisense
GGGAGTTCCAGGATCAGTTCATCCA 153 495 810 Antisense
Sequence Source Affymetrix Proprietary Database