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[184529_at]

Description F49E10.1 /REP_DB=WormBase Gene ID /WP=CE17844 /TR=Q20600 /GB=AAC69111.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 04      6grp_total > 24      4grp_total > 31      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 171
1 2 3 4 range
A 142.4
(3)
P 		75.1
(-0.3)
P 		18.4
(-3)
A 		101.7
(1)
A 		124
B 138.3
(3)
P 		68.6
(-1.4)
A 		43.9
(-3)
A 		68
(-1.5)
A 		94
C 189.4
(3)
P 		19.3
(-3)
A 		49.3
(-1.9)
A 		125.5
(0.7)
P 		170
avr 156.7
(3) 		54.3
(-2.1) 		37.2
(-3) 		98.4
(0.1) 		120

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 96
1 2 3 4 5 6 range
A 94.1
(0.3)
P 		110.4
(2)
P 		89.8
(-0.1)
P 		60.8
(-3)
P 		120.9
(3)
P 		94.1
(0.3)
A 		60
B 142.2
(3)
P 		129.2
(2.2)
P 		93.6
(-0)
P 		107
(0.8)
P 		46.4
(-3)
A 		53.4
(-2.6)
A 		96
C 132.5
(2.6)
P 		137.8
(3)
P 		133.5
(2.7)
P 		59
(-3)
A 		61.4
(-2.8)
P 		68.9
(-2.2)
P 		79
avr 122.9
(2.7) 		125.8
(3) 		105.6
(0.7) 		75.6
(-2.6) 		76.2
(-2.5) 		72.1
(-3) 		54
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 58
1 2 3 4 range
A 51.8
(3)
P 		16.3
(-1.5)
P 		4.8
(-3)
A 		14.9
(-1.7)
A 		47
B 44.1
(3)
P 		17
(-1.8)
A 		10.2
(-3)
A 		14.5
(-2.2)
A 		34
C 62.7
(3)
P 		5.5
(-3)
A 		17.2
(-1.8)
A 		21.2
(-1.4)
P 		57
avr 52.9
(3) 		12.9
(-2.7) 		10.7
(-3) 		16.9
(-2.1) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 38
1 2 3 4 5 6 range
A 17.3
(-2.5)
P 		39.3
(3)
P 		19.5
(-2)
P 		15.4
(-3)
P 		36.5
(2.3)
P 		22.9
(-1.1)
A 		24
B 49.5
(3)
P 		43
(1.7)
P 		22.7
(-2.3)
P 		28.4
(-1.1)
P 		18.9
(-3)
A 		19.9
(-2.8)
A 		31
C 49.1
(3)
P 		42.1
(1.9)
P 		34
(0.6)
P 		11.9
(-3)
A 		19.9
(-1.7)
P 		23.1
(-1.2)
P 		37
avr 38.6
(2.2) 		41.5
(3) 		25.4
(-1.2) 		18.6
(-3) 		25.1
(-1.3) 		22
(-2.1) 		23
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184529_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16581
Sequence Type Exemplar sequence
Representative Public ID F49E10.1 NCBI
Target Description F49E10.1 /REP_DB=WormBase Gene ID /WP=CE17844 /TR=Q20600 /GB=AAC69111.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076587(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076587 NCBI Caenorhabditis elegans F49E10.1 (F49E10.1) mRNA, complete cds. 9/11 A 
GENEFINDER00000040795 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:5898325:5901919:1 11/11 None
F49E10.1 ENSEMBL cdna:known chromosome:CEL140:X:5897349:5902097:1 gene:F49E10.1 9/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000040789 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5897348-5899907(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F49E10.1
Entrez Gene 180856 Entrez gene
SwissProt Q20600 EMBL-EBI
WormBase CE29965 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.9312.1.S1_AT similar to TBC1 domain family, member 16 cfa
DROSOPHILA_2:1626227_AT dm
DROSGENOME1:146159_AT dm
HG-U133_PLUS_2:228488_AT TBC1 domain family, member 16 hs
HG-U133B:228488_AT TBC1 domain family, member 16 hs
HU35KSUBA:RC_AA009527_AT TBC1 domain family, member 16 hs
HU35KSUBC:RC_N26714_AT TBC1 domain family, member 16 hs
HU35KSUBC:RC_F02618_AT TBC1 domain family, member 16 hs
U133_X3P:HS2.91973.3.S1_3P_S_AT TBC1 domain family, member 16 hs
U133_X3P:HS2.387410.1.S1_3P_S_AT TBC1 domain family, member 16 hs
U133_X3P:HS.91973.1.S1_3P_A_AT TBC1 domain family, member 16 hs
U133_X3P:HS.91973.0.S2_3P_AT TBC1 domain family, member 16 hs
U133_X3P:HS.26289.0.A1_3P_AT TBC1 domain family, member 16 hs
HG-U133_PLUS_2:1554701_A_AT TBC1 domain family, member 16 hs
HG-U133_PLUS_2:1558066_S_AT TBC1 domain family, member 16 hs
HG-U133A_2:222116_S_AT TBC1 domain family, member 16 hs
HG-U133_PLUS_2:222116_S_AT TBC1 domain family, member 16 hs
HG-U133A:222116_S_AT TBC1 domain family, member 16 hs
HG-U133_PLUS_2:222648_AT TBC1 domain family, member 16 hs
HG-U133B:222648_AT TBC1 domain family, member 16 hs
HG-U95B:43969_AT TBC1 domain family, member 16 hs
HG-U95B:44700_AT TBC1 domain family, member 16 hs
HG-U95B:46427_AT TBC1 domain family, member 16 hs
HG-U95D:75710_AT TBC1 domain family, member 16 hs
HG-U95D:84103_AT TBC1 domain family, member 16 hs
HG-U95E:84155_AT TBC1 domain family, member 16 hs
HG-U95C:63121_AT TBC1 domain family, member 16 hs
HG-U95D:78664_AT TBC1 domain family, member 16 hs
HG-U95D:83885_AT TBC1 domain family, member 16 hs
HU35KSUBC:RC_AA011341_AT TBC1 domain family, member 16 hs
HU35KSUBC:RC_N73465_AT TBC1 domain family, member 16 hs
MG-U74BV2:110258_AT TBC1 domain family, member 16 mm
MOE430B:1435763_AT TBC1 domain family, member 16 mm
MOUSE430_2:1435763_AT TBC1 domain family, member 16 mm
MOE430B:1442149_AT TBC1 domain family, member 16 mm
MOUSE430_2:1442149_AT TBC1 domain family, member 16 mm
Protein Similarities
Method ID Description E-Value
blast AAC69111 Hypothetical protein F49E10.1 [Caenorhabditis elegans] ref|NP_508988.2| F49E10.1 [Caenorhabditis elegans] 0.0
blast CAE68817 Hypothetical protein CBG14774 [Caenorhabditis briggsae] 0.0
blast XP_221188 PREDICTED: similar to TBC1 domain family, member 16 [Rattus norvegicus] 1.0E-127
blast AAH79700 MGC68883 protein [Xenopus laevis] gb|AAH57720.1| MGC68883 protein [Xenopus laevis] 1.0E-114
Sequence
Target Sequence 
>C. ELEGANS:184529_AT
gtcttttctcggtcaatcttggtaatggcaccgctaattatacactttcactttatggtg
tttcaggaaaaatgcggagcatgcgtgtgttctactcgaacacggagtgcacatccgggc
aattagtgatcgccactcttgactcgcattacaagatccttcattttcatcatggtggac
tggacaaactcgcctacatctttgaacaatggtcgtcaataaaggcgcgaagcgtgcaag
aaggctctccgtcagcaatagccgataagcagcttataatatgccagcctgcagtcacaa
a

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCTTTTCTCGGTCAATCTTGGTAA 483 469 920 Antisense
GGCACCGCTAATTATACACTTTCAC 92 537 946 Antisense
GAGCATGCGTGTGTTCTACTCGAAC 491 387 996 Antisense
GAACACGGAGTGCACATCCGGGCAA 321 351 1017 Antisense
AATTAGTGATCGCCACTCTTGACTC 505 175 1040 Antisense
GACTCGCATTACAAGATCCTTCATT 338 375 1060 Antisense
ATGGTGGACTGGACAAACTCGCCTA 525 49 1091 Antisense
AAACTCGCCTACATCTTTGAACAAT 489 125 1105 Antisense
GAAGCGTGCAAGAAGGCTCTCCGTC 192 343 1148 Antisense
GCTCTCCGTCAGCAATAGCCGATAA 59 297 1163 Antisense
TAATATGCCAGCCTGCAGTCACAAA 330 633 1196 Antisense
Sequence Source Affymetrix Proprietary Database