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[184522_at]

Description F49F1.12 /REP_DB=WormBase Gene ID /WP=CE17095 /TR=Q9GZD7 /GB=AAF99960.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 96      4grp_total > 28      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 77
1 2 3 4 range
A 51.9
(-0.5)
A 		26.9
(-3)
A 		32.1
(-2.5)
A 		87.9
(3)
A 		61
B 24.9
(-1.9)
A 		68.6
(3)
A 		15.2
(-3)
A 		31.3
(-1.2)
A 		53
C 40.6
(-0.3)
A 		63.7
(1.8)
A 		10.8
(-3)
A 		77.4
(3)
A 		67
avr 39.1
(-0.4) 		53.1
(1.4) 		19.4
(-3) 		65.5
(3) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 115
1 2 3 4 5 6 range
A 10.4
(-3)
A 		45.2
(-0.9)
A 		17.5
(-2.6)
A 		45.2
(-0.9)
A 		70
(0.6)
A 		108.4
(3)
A 		98
B 48.2
(-2)
A 		78
(0.1)
A 		32.7
(-3)
A 		47.8
(-2)
A 		84.2
(0.5)
A 		121.5
(3)
A 		89
C 8.2
(-2.9)
A 		54.9
(-0.1)
A 		6.7
(-3)
A 		107.3
(3)
A 		104.5
(2.8)
A 		86.5
(1.8)
A 		101
avr 22.3
(-2.8) 		59.4
(-0.2) 		19
(-3) 		66.8
(0.3) 		86.2
(1.7) 		105.5
(3) 		87
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 18.9
(3)
A 		5.8
(-3)
A 		8.4
(-1.8)
A 		12.9
(0.3)
A 		13
B 7.9
(-1)
A 		16.9
(3)
A 		3.5
(-3)
A 		6.7
(-1.6)
A 		13
C 13.4
(1)
A 		18.3
(3)
A 		3.8
(-3)
A 		13.1
(0.8)
A 		15
avr 13.4
(2.8) 		13.7
(3) 		5.2
(-3) 		10.9
(1) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 44
1 2 3 4 5 6 range
A 1.9
(-3)
A 		16.1
(0.5)
A 		3.8
(-2.5)
A 		11.5
(-0.6)
A 		21.1
(1.7)
A 		26.4
(3)
A 		25
B 16.8
(-1.6)
A 		26
(-0.1)
A 		7.9
(-3)
A 		12.7
(-2.2)
A 		34.2
(1.2)
A 		45.3
(3)
A 		37
C 3.1
(-2.7)
A 		16.7
(-0.2)
A 		1.7
(-3)
A 		21.6
(0.7)
A 		33.8
(3)
A 		28.9
(2.1)
A 		32
avr 7.3
(-2.4) 		19.6
(0.1) 		4.5
(-3) 		15.3
(-0.8) 		29.7
(2.2) 		33.5
(3) 		29
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184522_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10844
Sequence Type Exemplar sequence
Representative Public ID F49F1.12 NCBI
Target Description F49F1.12 /REP_DB=WormBase Gene ID /WP=CE17095 /TR=Q9GZD7 /GB=AAF99960.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068092(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068092 NCBI Caenorhabditis elegans F49F1.12 (F49F1.12) mRNA, complete cds. 11/11 None
SNAP00000034596 ENSEMBL cdna:SNAP chromosome:CEL140:IV:4141736:4143012:-1 11/11 None
GENEFINDER00000034599 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:4135546:4143012:-1 11/11 None
F49F1.12 ENSEMBL cdna:known chromosome:CEL140:IV:4141719:4142733:-1 gene:F49F1.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4141724-4142739(-) 62.36 100.0
Public Domain and Genome References
Gene Title
Ensembl F49F1.12
Entrez Gene 186068 Entrez gene
SwissProt Q9GZD7 EMBL-EBI
WormBase CE17095 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF99966 Hypothetical protein F49F1.10 [Caenorhabditis elegans] ref|NP_500491.1| F49F1.10 [Caenorhabditis elegans] 7.0E-92
blast AAF99967 Hypothetical protein F49F1.11 [Caenorhabditis elegans] ref|NP_500492.1| F49F1.11 [Caenorhabditis elegans] 5.0E-81
blast AAF99965 Hypothetical protein F49F1.9 [Caenorhabditis elegans] ref|NP_500490.1| F49F1.9 [Caenorhabditis elegans] 9.0E-81
blast CAB04392 Hypothetical protein F46A8.6 [Caenorhabditis elegans] ref|NP_492886.2| F46A8.6 [Caenorhabditis elegans] 4.0E-35
blast AAF99960 Hypothetical protein F49F1.12 [Caenorhabditis elegans] ref|NP_500493.1| F49F1.12 [Caenorhabditis elegans] 1.0E-21
blast CAB55058 Hypothetical protein Y50E8A.10 [Caenorhabditis elegans] ref|NP_506647.1| Y50E8A.10 [Caenorhabditis elegans] 8.0E-15
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000034596 1 7-26
GENEFINDER00000034599 1 7-26
Sequence
Target Sequence 
>C. ELEGANS:184522_AT
gaggtggtggcggcaatggatccggatgtcaagtctacgcagagatcagcgaaccaaaac
cgccaaaagcggcaaagatgtggcaccagccgctgctcagactcctgctcaggatcaagg
tccactccgatcccagcctgtcggccttccaatggcacctggaatcaaatcgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGGTGGTGGCGGCAATGGATCCGG 225 403 157 Antisense
GGTGGCGGCAATGGATCCGGATGTC 108 499 162 Antisense
ATGGATCCGGATGTCAAGTCTACGC 329 51 172 Antisense
GATCCGGATGTCAAGTCTACGCAGA 119 417 175 Antisense
CAAAACCGCCAAAAGCGGCAAAGAT 588 183 211 Antisense
CGGCAAAGATGTGGCACCAGCCGCT 437 241 226 Antisense
TGCTCAGACTCCTGCTCAGGATCAA 24 581 250 Antisense
TCCTGCTCAGGATCAAGGTCCACTC 659 591 259 Antisense
TCAGGATCAAGGTCCACTCCGATCC 405 623 265 Antisense
GGCCTTCCAATGGCACCTGGAATCA 5 545 299 Antisense
CAATGGCACCTGGAATCAAATCGAT 421 181 306 Antisense
Sequence Source Affymetrix Proprietary Database