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[184521_at]

Description Y73F8A.33 /REP_DB=WormBase Gene ID /WP=CE24651 /TR=Q9NA44 /GB=CAB60561.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 40      4grp_total > 49      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 76
1 2 3 4 range
A 39.7
(-0.5)
A 		75.8
(3)
A 		59.9
(1.5)
A 		13.2
(-3)
A 		63
B 17.3
(-3)
A 		82.1
(2.8)
A 		84.4
(3)
A 		42.9
(-0.7)
A 		67
C 8.7
(-3)
A 		63.3
(3)
A 		32.3
(-0.4)
A 		31.6
(-0.5)
A 		55
avr 21.9
(-3) 		73.7
(3) 		58.9
(1.3) 		29.2
(-2.2) 		52

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 75
1 2 3 4 5 6 range
A 81.6
(3)
A 		33.5
(-3)
A 		65.9
(1)
A 		51
(-0.8)
A 		54.4
(-0.4)
A 		55.7
(-0.2)
A 		48
B 43.9
(1.5)
A 		54.8
(3)
A 		13.5
(-2.6)
A 		20.9
(-1.6)
A 		10.3
(-3)
A 		21.6
(-1.5)
A 		45
C 6.3
(-3)
A 		69.5
(2.5)
A 		72.7
(2.8)
A 		75.1
(3)
A 		7.7
(-2.9)
A 		44.2
(0.3)
A 		69
avr 43.9
(1.2) 		52.6
(3) 		50.7
(2.6) 		49
(2.2) 		24.1
(-3) 		40.5
(0.5) 		29
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 14.5
(2.2)
A 		16.5
(3)
A 		15.7
(2.7)
A 		1.9
(-3)
A 		15
B 5.5
(-3)
A 		20.3
(3)
A 		19.6
(2.7)
A 		9.1
(-1.5)
A 		15
C 2.9
(-3)
A 		18.2
(3)
A 		11.3
(0.3)
A 		5.3
(-2.1)
A 		15
avr 7.6
(-2) 		18.3
(3) 		15.5
(1.7) 		5.4
(-3) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 15
(1.1)
A 		11.9
(-3)
A 		14.4
(0.3)
A 		12.9
(-1.7)
A 		16.4
(3)
A 		13.6
(-0.7)
A 		5
B 15.3
(1.8)
A 		18.2
(3)
A 		3.3
(-3)
A 		5.5
(-2.1)
A 		4.2
(-2.6)
A 		8.1
(-1.1)
A 		15
C 2.3
(-3)
A 		21.2
(3)
A 		18.5
(2.1)
A 		15.1
(1.1)
A 		2.5
(-2.9)
A 		14.8
(1)
A 		19
avr 10.9
(-1) 		17.1
(3) 		12.1
(-0.2) 		11.2
(-0.8) 		7.7
(-3) 		12.2
(-0.1) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184521_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8765
Sequence Type Exemplar sequence
Representative Public ID Y73F8A.33 NCBI
Target Description Y73F8A.33 /REP_DB=WormBase Gene ID /WP=CE24651 /TR=Q9NA44 /GB=CAB60561.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070467(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070467 NCBI Caenorhabditis elegans Y73F8A.33 (Y73F8A.33) mRNA, complete cds. 11/11 None
SNAP00000030837 ENSEMBL cdna:SNAP chromosome:CEL140:IV:15520086:15520894:1 11/11 None
GENEFINDER00000030916 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:15515457:15520894:1 11/11 None
Y73F8A.33 ENSEMBL cdna:known chromosome:CEL140:IV:15515457:15520894:1 gene:Y73F8A.33 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:15515462-15520900(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y73F8A.33
Entrez Gene 190689 Entrez gene
SwissProt Q9NA44 EMBL-EBI
WormBase CE24651 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB60561 Hypothetical protein Y73F8A.33 [Caenorhabditis elegans] ref|NP_502868.1| Y73F8A.33 [Caenorhabditis elegans] 0.0
blast CAB60561 Hypothetical protein Y73F8A.33 [Caenorhabditis elegans] ref|NP_502868.1| Y73F8A.33 [Caenorhabditis elegans] 1.0E-118
blast AAB94979 Hypothetical protein F52C6.14 [Caenorhabditis elegans] ref|NP_494132.1| F52C6.14 [Caenorhabditis elegans] 3.0E-74
blast AAF59578 Hypothetical protein Y54G2A.20 [Caenorhabditis elegans] ref|NP_500269.1| Y54G2A.20 [Caenorhabditis elegans] 7.0E-29
Sequence
Target Sequence 
>C. ELEGANS:184521_AT
aatgcaccttcgacgatgggcttcacgtatgaggctgaaaaattgtacaatgaatttatg
caggaaaatgacgtgttgtgtaggaagatgaggcaatataagaagtcaagtagcgccgag
tccctggaaactatgcgaaaaaatctcgaaattgttcgtgaagcgaacaagaatactcct
ggaaccagccaggtcttaacagttcgccgagatgctcatcttccatatagaacagcaacg
cagactatgaatcgtattgtgcgacgcaaaaagggagacagaaagcgatcgaataaactt
ctagcttcttctttgccacccaagaaagacgtgtctatttgcagcaaatgccactttgcg
gatcccgttcttgattcggatattcacgagacagacttcgaatcaatcgtcactgaatgg
ctccgatgctctacatgcaagctgcccgtacacttcgtctgctcacatgatggttgcccc
agctgtggtggcactttggagtcatacttacctgacgaagtggacacgcagaactcatct
tccga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATGCACCTTCGACGATGGGCTTCA 600 165 2023 Antisense
CGCCGAGTCCCTGGAAACTATGCGA 317 257 2136 Antisense
AACCAGCCAGGTCTTAACAGTTCGC 242 129 2205 Antisense
AACAGTTCGCCGAGATGCTCATCTT 171 137 2220 Antisense
AATAAACTTCTAGCTTCTTCTTTGC 75 173 2314 Antisense
CGGATCCCGTTCTTGATTCGGATAT 2 245 2381 Antisense
TCACTGAATGGCTCCGATGCTCTAC 300 627 2432 Antisense
CACTTCGTCTGCTCACATGATGGTT 558 195 2473 Antisense
TCACATGATGGTTGCCCCAGCTGTG 703 627 2485 Antisense
GGCACTTTGGAGTCATACTTACCTG 499 535 2512 Antisense
TGGACACGCAGAACTCATCTTCCGA 562 547 2543 Antisense
Sequence Source Affymetrix Proprietary Database