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[184514_at] glutathione S-transferase

Description F56B3.10 /REP_DB=WormBase Gene ID /WP=CE27168 /TR=O45115 /GB=AAK29835.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 70      6grp_high1_10_10 > 07      4grp_highest_8_8 > 53      4grp_high_8_8 > 09      6grp_highest_nonorm > 70      6grp_total > 08      4grp_total > 23      6grp_highest_sub > De      6grp_cgc > 11      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 341
1 2 3 4 range
A 477.2
(3)
P 		184.8
(-2.4)
P 		150
(-3)
P 		382
(1.3)
P 		327
B 483.9
(3)
P 		240.9
(-2.1)
P 		199.1
(-3)
P 		433.9
(1.9)
P 		285
C 491.2
(3)
P 		226.9
(-1.9)
P 		166.4
(-3)
P 		399.4
(1.3)
P 		325
avr 484.1
(3) 		217.5
(-2.1) 		171.8
(-3) 		405.1
(1.5) 		312

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 361
1 2 3 4 5 6 range
A 512.4
(3)
P 		336.1
(-0.4)
P 		220.8
(-2.6)
P 		316.2
(-0.7)
P 		261.5
(-1.8)
P 		198.3
(-3)
P 		314
B 549.4
(3)
P 		292.1
(-1.3)
P 		272.6
(-1.6)
P 		249.7
(-2)
P 		252.8
(-1.9)
P 		188.1
(-3)
P 		361
C 480.6
(3)
P 		291.3
(-1)
P 		228.7
(-2.3)
P 		296.5
(-0.8)
P 		222.6
(-2.4)
P 		193.6
(-3)
P 		287
avr 514.1
(3) 		306.5
(-0.9) 		240.7
(-2.1) 		287.5
(-1.2) 		245.6
(-2) 		193.3
(-3) 		321
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 135
1 2 3 4 range
A 173.7
(3)
P 		40.1
(-3)
P 		39.2
(-3)
P 		55.9
(-2.3)
P 		135
B 154.3
(3)
P 		59.5
(-2.3)
P 		46.1
(-3)
P 		92.5
(-0.4)
P 		108
C 162.6
(3)
P 		65.2
(-2.6)
P 		58
(-3)
P 		67.3
(-2.5)
P 		105
avr 163.5
(3) 		54.9
(-2.6) 		47.8
(-3) 		71.9
(-1.8) 		116

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 143
1 2 3 4 5 6 range
A 94.5
(0.9)
P 		119.5
(3)
P 		48.1
(-3)
P 		80.2
(-0.3)
P 		78.9
(-0.4)
P 		48.3
(-3)
P 		71
B 191.2
(3)
P 		97.2
(-1.5)
P 		66.2
(-3)
P 		66.1
(-3)
P 		102.7
(-1.2)
P 		70.1
(-2.8)
P 		125
C 178.1
(3)
P 		88.9
(-1.5)
P 		58.2
(-3)
P 		59.6
(-2.9)
P 		72
(-2.3)
P 		64.8
(-2.7)
P 		120
avr 154.6
(3) 		101.9
(-0.3) 		57.5
(-3) 		68.6
(-2.3) 		84.5
(-1.3) 		61.1
(-2.8) 		97
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184514_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10864
Sequence Type Exemplar sequence
Representative Public ID F56B3.10 NCBI
Target Description F56B3.10 /REP_DB=WormBase Gene ID /WP=CE27168 /TR=O45115 /GB=AAK29835.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067580(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067580 NCBI Caenorhabditis elegans Glutathione S-Transferase family member (gst-40) (gst-40) mRNA, complete cds. 11/11 A 
SNAP00000041560 ENSEMBL cdna:SNAP chromosome:CEL140:IV:760618:767729:1 11/11 None
GENEFINDER00000041575 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:760618:762295:1 11/11 None
F56B3.10 ENSEMBL cdna:known chromosome:CEL140:IV:760610:762342:1 gene:F56B3.10 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:761615-762301(+) 100.0 100.0
Public Domain and Genome References
Gene Title glutathione S-transferase
Gene Symbol gst-40
Ensembl F56B3.10
Entrez Gene 176900 Entrez gene
SwissProt O45115 EMBL-EBI
WormBase CE27168 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK29835 Glutathione s-transferase protein 40 [Caenorhabditis elegans] ref|NP_499981.1| Glutathione S-Transferase family member (gst-40) [Caenorhabditis elegans] 1.0E-125
blast CAE65653 Hypothetical protein CBG10717 [Caenorhabditis briggsae] 1.0E-105
blast CAB02288 Hypothetical protein F37B1.2 [Caenorhabditis elegans] ref|NP_496862.1| Glutathione S-Transferase family member (gst-12) [Caenorhabditis elegans] 8.0E-21
Protein Domains
Database ID Description E-Value
Pfam IPR004046 EMBL-EBI Glutathione S-transferase, C-terminal 1.8E-9
Pfam IPR004045 EMBL-EBI Glutathione S-transferase, N-terminal 3.7E-14
Sequence
Target Sequence 
>C. ELEGANS:184514_AT
atgaaacagctcccttttatcgaaatcgatggaggaaagactactctctgtcagacggta
tctatttgccgctacctggcaaagtcaattcagccggatagatggttcggcggagctacc
aaaaccgattcggccaaagtggacatgatggctgatgggtttgctgatttgttccagctt
gccgcaatggctaagtatgcaccggatcccatcaaggattccatgatggggacctacaaa
gagtccattggtccaaagctggaaaatatgcaagatttgttgaaaaaaagcaaaggagag
tacttcgtgggaaaatcgatccattggtgtgatgtctacattcttggggtgttgcaggca
ttggatgagagcgacgacggtgtcttcgacaatcttcccgaactccgcgagtactatctc
agaatgcgcaacctgccggaatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGAAACAGCTCCCTTTTATCGAAA 572 43 13 Antisense
GAAAGACTACTCTCTGTCAGACGGT 375 359 47 Antisense
AGACGGTATCTATTTGCCGCTACCT 286 71 65 Antisense
GGATAGATGGTTCGGCGGAGCTACC 621 507 108 Antisense
GAGCTACCAAAACCGATTCGGCCAA 474 387 125 Antisense
GGCTGATGGGTTTGCTGATTTGTTC 636 539 162 Antisense
TGTTCCAGCTTGCCGCAATGGCTAA 457 563 182 Antisense
TGGCTAAGTATGCACCGGATCCCAT 665 543 200 Antisense
TGAGAGCGACGACGGTGTCTTCGAC 665 567 378 Antisense
AACTCCGCGAGTACTATCTCAGAAT 398 141 413 Antisense
TCAGAATGCGCAACCTGCCGGAATT 342 607 431 Antisense
Sequence Source Affymetrix Proprietary Database