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[184509_at] DUF-216 family

Description T25E12.11 /REP_DB=WormBase Gene ID /WP=CE16472 /TR=O45825 /GB=CAB04829.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 03      4grp_total > 35      6grp_cgc > 40      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 39
1 2 3 4 range
A 22.6
(0.2)
A 		16.8
(-0.7)
A 		1.9
(-3)
A 		40.9
(3)
A 		39
B 2.1
(-3)
A 		15.8
(3)
A 		13.9
(2.2)
A 		10.2
(0.5)
A 		14
C 18.1
(-0.5)
A 		38.2
(3)
A 		13.1
(-1.4)
A 		4
(-3)
A 		34
avr 14.3
(-1) 		23.6
(3) 		9.6
(-3) 		18.4
(0.8) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 9.1
(-0.8)
A 		19.3
(2)
A 		1.1
(-3)
A 		23
(3)
P 		12
(-0)
A 		14.5
(0.7)
A 		22
B 21.2
(1.9)
P 		25.4
(3)
P 		1.9
(-3)
A 		13.7
(0)
A 		2.3
(-2.9)
A 		12.2
(-0.4)
A 		24
C 24.3
(2.9)
A 		1.6
(-3)
A 		17.6
(1.2)
A 		24.5
(3)
A 		1.6
(-3)
A 		2.8
(-2.7)
A 		23
avr 18.2
(2.1) 		15.4
(1) 		6.9
(-2.4) 		20.4
(3) 		5.3
(-3) 		9.8
(-1.2) 		15
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 8.2
(3)
A 		3.6
(-0.6)
A 		0.5
(-3)
A 		6
(1.3)
A 		8
B 0.7
(-3)
A 		3.9
(3)
A 		3.2
(1.7)
A 		2.2
(-0.2)
A 		3
C 6
(0.1)
A 		11
(3)
A 		4.6
(-0.7)
A 		0.7
(-3)
A 		10
avr 5
(0.9) 		6.2
(3) 		2.8
(-3) 		3
(-2.6) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9
1 2 3 4 5 6 range
A 1.7
(-1.7)
A 		6.9
(3)
A 		0.2
(-3)
A 		5.8
(2)
P 		3.6
(0)
A 		3.5
(-0)
A 		7
B 7.4
(2.2)
P 		8.4
(3)
P 		0.5
(-3)
A 		3.6
(-0.6)
A 		0.9
(-2.7)
A 		4.5
(0)
A 		8
C 9
(3)
A 		0.5
(-3)
A 		4.5
(-0.2)
A 		4.9
(0.1)
A 		0.5
(-3)
A 		0.9
(-2.7)
A 		9
avr 6
(3) 		5.3
(2) 		1.7
(-3) 		4.8
(1.3) 		1.7
(-3) 		3
(-1.2) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184509_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13414
Sequence Type Exemplar sequence
Representative Public ID T25E12.11 NCBI
Target Description T25E12.11 /REP_DB=WormBase Gene ID /WP=CE16472 /TR=O45825 /GB=CAB04829.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074831(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074831 NCBI Caenorhabditis elegans Serpentine Receptor, class Z family member (srz-100) (srz-100) mRNA, complete cds. 10/11 None
GENEFINDER00000017075 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:16730957:16735728:1 10/11 None
T25E12.11 ENSEMBL cdna:known chromosome:CEL140:V:16734481:16735728:1 gene:T25E12.11 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000017065 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16731951-16735728(+) 100.0 100.0
Public Domain and Genome References
Gene Title DUF-216 family
Gene Symbol srz-100
Ensembl T25E12.11
Entrez Gene 188901 Entrez gene
SwissProt O45825 EMBL-EBI
WormBase CE33839 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04829 Hypothetical protein T25E12.11 [Caenorhabditis elegans] ref|NP_507232.3| Serpentine Receptor, class Z family member (srz-100) [Caenorhabditis elegans] 1.0E-164
blast CAB04829 Hypothetical protein T25E12.11 [Caenorhabditis elegans] ref|NP_507232.3| Serpentine Receptor, class Z family member (srz-100) [Caenorhabditis elegans] 1.0E-152
blast CAD89751 Hypothetical protein T25E12.13 [Caenorhabditis elegans] ref|NP_001024156.1| Serpentine Receptor, class Z family member (srz-99) [Caenorhabditis elegans] 1.0E-129
blast CAD89750 Hypothetical protein T25E12.12 [Caenorhabditis elegans] ref|NP_001024155.1| T25E12.12 [Caenorhabditis elegans] 1.0E-121
blast CAD89751 Hypothetical protein T25E12.13 [Caenorhabditis elegans] ref|NP_001024156.1| Serpentine Receptor, class Z family member (srz-99) [Caenorhabditis elegans] 1.0E-121
blast CAA20970 Hypothetical protein Y102A5C.33 [Caenorhabditis elegans] ref|NP_507299.1| Serpentine Receptor, class Z family member (srz-95) [Caenorhabditis elegans] 3.0E-67
Protein Domains
Database ID Description E-Value
Pfam IPR003845 EMBL-EBI Protein of unknown function DUF216 1.1E-7
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 6.5E-47
Pfam IPR003845 EMBL-EBI Protein of unknown function DUF216 1.1E-7
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507232.3 7 20-42,62-84,94-113,118-140,150-169,190-212,232-254
T25E12.11 7 20-42,62-84,94-113,118-140,150-169,190-212,232-254
GENEFINDER00000017075 14 228-250,270-292,304-335,355-377,389-411,421-443,450-467,487-509,516-538,543-565,578-600,615-637,650-672,687-709
Sequence
Target Sequence 
>C. ELEGANS:184509_AT
tcgatatttatctttcacattttacaacaaacaatccatttactatttcccaggatttca
tacggaagtatatcacacctcttaacgtgtttttcatttcactggatgctgcagcgttta
tgttgcatttccttcgtcagtactgccttctaagtacagaaaaagttcaaattttcatag
cgtgcaatgaggtattatatatgtttttgaacgttgtaatatcgtttctaactccattta
tctacattccaattatgattgatataaggaaaaatagacatcttcactccccacaacaca
tgtttctacacaattacatattttttcagagctttctagtgatcattctacccttttgtt
ttgaaactggaaactcttctattgaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGATATTTATCTTTCACATTTTAC 275 603 1556 Antisense
TCCATTTACTATTTCCCAGGATTTC 180 591 1590 Antisense
GGAAGTATATCACACCTCTTAACGT 138 531 1619 Antisense
TAACGTGTTTTTCATTTCACTGGAT 153 639 1638 Antisense
TTCACTGGATGCTGCAGCGTTTATG 488 687 1653 Antisense
GCGTTTATGTTGCATTTCCTTCGTC 84 291 1669 Antisense
CTTCGTCAGTACTGCCTTCTAAGTA 201 219 1687 Antisense
GTTGTAATATCGTTTCTAACTCCAT 663 437 1768 Antisense
GAGCTTTCTAGTGATCATTCTACCC 586 387 1884 Antisense
GATCATTCTACCCTTTTGTTTTGAA 603 419 1896 Antisense
GAAACTGGAAACTCTTCTATTGAAT 666 357 1918 Antisense
Sequence Source Affymetrix Proprietary Database