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[184495_at]

Description F45C12.9 /REP_DB=WormBase Gene ID /WP=CE10452 /TR=O16738 /GB=AAB66208.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 63      4grp_total > 0      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 19.2
(-1.1)
A 		10.3
(-3)
A 		38.5
(3)
A 		29.2
(1)
A 		28
B 15.5
(-0.9)
A 		8.4
(-2.3)
A 		4.6
(-3)
A 		36.2
(3)
A 		32
C 12.9
(3)
A 		10.4
(0.1)
A 		7.7
(-3)
A 		12.3
(2.3)
A 		5
avr 15.9
(-0.7) 		9.7
(-3) 		16.9
(-0.3) 		25.9
(3) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 8.8
(-2.3)
A 		3.1
(-3)
A 		26.3
(-0.1)
A 		50.4
(3)
A 		25
(-0.2)
A 		3.2
(-3)
A 		47
B 3.5
(-3)
A 		29.2
(3)
A 		17.6
(0.3)
A 		6.8
(-2.2)
A 		5.5
(-2.5)
A 		19.4
(0.7)
A 		26
C 12.6
(-1.1)
A 		2.6
(-2.9)
A 		36.6
(3)
A 		1.8
(-3)
A 		16.6
(-0.4)
A 		13.5
(-1)
A 		35
avr 8.3
(-3) 		11.6
(-1.9) 		26.8
(3) 		19.7
(0.7) 		15.7
(-0.6) 		12
(-1.8) 		19
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 7
(0.6)
A 		2.2
(-3)
A 		10.1
(3)
A 		4.3
(-1.4)
A 		8
B 4.9
(0.5)
A 		2.1
(-2.1)
A 		1.1
(-3)
A 		7.7
(3)
A 		7
C 4.3
(3)
A 		3
(-0.5)
A 		2.7
(-1.4)
A 		2.1
(-3)
A 		2
avr 5.4
(3) 		2.4
(-3) 		4.6
(1.4) 		4.7
(1.6) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 12
1 2 3 4 5 6 range
A 1.6
(-2.6)
A 		1.1
(-2.9)
A 		5.7
(-0.6)
A 		12.8
(3)
A 		7.5
(0.4)
A 		0.8
(-3)
A 		12
B 1.2
(-3)
A 		9.7
(3)
A 		4.3
(-0.8)
A 		1.8
(-2.6)
A 		2.2
(-2.3)
A 		7.2
(1.2)
A 		9
C 4.7
(-0.1)
A 		0.8
(-2.7)
A 		9.3
(3)
A 		0.4
(-3)
A 		5.4
(0.4)
A 		4.5
(-0.2)
A 		9
avr 2.5
(-3) 		3.9
(-0.8) 		6.4
(3) 		5
(0.8) 		5
(0.8) 		4.2
(-0.4) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184495_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5237
Sequence Type Exemplar sequence
Representative Public ID F45C12.9 NCBI
Target Description F45C12.9 /REP_DB=WormBase Gene ID /WP=CE10452 /TR=O16738 /GB=AAB66208.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061650(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061650 NCBI Caenorhabditis elegans F45C12.9 (F45C12.9) mRNA, complete cds. 11/11 None
SNAP00000033185 ENSEMBL cdna:SNAP chromosome:CEL140:II:1708435:1709769:1 11/11 None
F45C12.9 ENSEMBL cdna:known chromosome:CEL140:II:1708435:1709699:1 gene:F45C12.9 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000033208 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:1708433-1709698(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F45C12.9
Entrez Gene 185771 Entrez gene
SwissProt O16738 EMBL-EBI
WormBase CE10452 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB66208 Hypothetical protein F45C12.9 [Caenorhabditis elegans] ref|NP_494051.1| F45C12.9 [Caenorhabditis elegans] 9.0E-82
blast AAB66208 Hypothetical protein F45C12.9 [Caenorhabditis elegans] ref|NP_494051.1| F45C12.9 [Caenorhabditis elegans] 3.0E-81
blast NP_494449 Y25C1A.2 [Caenorhabditis elegans] gb|AAD12834.1| Hypothetical protein Y25C1A.2 [Caenorhabditis elegans] 1.0E-25
blast NP_494449 Y25C1A.2 [Caenorhabditis elegans] gb|AAD12834.1| Hypothetical protein Y25C1A.2 [Caenorhabditis elegans] 2.0E-21
blast AAG00001 Hypothetical protein R03H10.5 [Caenorhabditis elegans] ref|NP_494732.1| R03H10.5 [Caenorhabditis elegans] 1.0E-15
blast AAB70964 Hypothetical protein C08E3.3 [Caenorhabditis elegans] ref|NP_494007.1| C08E3.3 [Caenorhabditis elegans] 4.0E-11
Protein Domains
Database ID Description E-Value
Pfam IPR003326 EMBL-EBI Protein of unknown function DUF130 6.9E-11
Pfam IPR003326 EMBL-EBI Protein of unknown function DUF130 6.9E-11
Sequence
Target Sequence 
>C. ELEGANS:184495_AT
aagccttgtggtttttgtgtctgtcgtggccatgattttctttgccgaacacggttgttc
agattcttgcacgtgtccggatatccgaagtattttttatacgtggagcaataactcaat
attgtactcggaaggacctggatgcattagaaacttgacctgctcaacagatacttgcat
tctcaattattattatctttgcaatcttttcaacttctatgggatagtttgcgaggatgg
tcaatggtacgctaccaagtatccatacggaatttcctactttgccataactgtgacatg
gtacacgatggatgaaacagtggaagggctcctccacattgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGCCTTGTGGTTTTTGTGTCTGTC 8 149 18 Antisense
TCTGTCGTGGCCATGATTTTCTTTG 167 609 37 Antisense
ATGATTTTCTTTGCCGAACACGGTT 644 43 49 Antisense
AACACGGTTGTTCAGATTCTTGCAC 509 135 65 Antisense
TTCTTGCACGTGTCCGGATATCCGA 77 689 81 Antisense
GTACTCGGAAGGACCTGGATGCATT 259 459 141 Antisense
GAAACTTGACCTGCTCAACAGATAC 93 357 167 Antisense
ATCTTTGCAATCTTTTCAACTTCTA 23 33 212 Antisense
GAGGATGGTCAATGGTACGCTACCA 133 403 250 Antisense
ATACGGAATTTCCTACTTTGCCATA 586 19 282 Antisense
CAGTGGAAGGGCTCCTCCACATTGT 502 207 335 Antisense
Sequence Source Affymetrix Proprietary Database