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[184473_at]

Description Y53C10A.2 /REP_DB=WormBase Gene ID /WP=CE22370 /TR=Q9XW55 /GB=CAA22136.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 87      4grp_total > 01      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 93
1 2 3 4 range
A 6.3
(-3)
A 		23.9
(-0.1)
A 		8.5
(-2.6)
A 		43.1
(3)
A 		37
B 34
(3)
A 		16.9
(-1.7)
A 		22.4
(-0.2)
A 		12.3
(-3)
A 		22
C 5.6
(-2.9)
A 		4.6
(-3)
A 		30.3
(-1.3)
A 		98
(3)
A 		93
avr 15.3
(-3) 		15.1
(-3) 		20.4
(-2.1) 		51.1
(3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 39
1 2 3 4 5 6 range
A 30.5
(2.5)
A 		20.5
(0)
A 		8.5
(-3)
A 		24.2
(0.9)
A 		17.3
(-0.8)
A 		32.5
(3)
A 		24
B 5
(-3)
A 		24
(-0)
A 		27.5
(0.5)
A 		17.6
(-1)
A 		30
(0.9)
A 		43.6
(3)
A 		39
C 28.3
(1.5)
A 		23.3
(-0.1)
A 		14
(-3)
A 		33.1
(3)
A 		29.4
(1.8)
A 		28.7
(1.6)
A 		19
avr 21.3
(-1.5) 		22.6
(-1.1) 		16.7
(-3) 		25
(-0.3) 		25.6
(-0.1) 		34.9
(3) 		18
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 2.3
(-2.9)
A 		5.2
(1.4)
A 		2.2
(-3)
A 		6.3
(3)
A 		4
B 10.8
(3)
A 		4.2
(-1.8)
A 		5.2
(-1.1)
A 		2.6
(-3)
A 		8
C 1.9
(-2.8)
A 		1.3
(-3)
A 		10.5
(0.6)
A 		16.5
(3)
A 		15
avr 5
(-1.3) 		3.6
(-3) 		6
(-0.1) 		8.5
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 5.6
(0.7)
A 		7.3
(2.4)
A 		1.9
(-3)
A 		6.1
(1.2)
A 		5.2
(0.3)
A 		7.9
(3)
A 		6
B 1.7
(-3)
A 		8
(-0.4)
A 		6.7
(-0.9)
A 		4.7
(-1.8)
A 		12.2
(1.3)
A 		16.2
(3)
A 		15
C 10.5
(3)
A 		7.1
(0)
A 		3.6
(-3)
A 		6.7
(-0.3)
A 		9.5
(2.1)
A 		9.6
(2.2)
A 		7
avr 5.9
(-1.5) 		7.5
(-0.1) 		4.1
(-3) 		5.8
(-1.6) 		9
(1.1) 		11.2
(3) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184473_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1434
Sequence Type Exemplar sequence
Representative Public ID Y53C10A.2 NCBI
Target Description Y53C10A.2 /REP_DB=WormBase Gene ID /WP=CE22370 /TR=Q9XW55 /GB=CAA22136.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060632(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060632 NCBI Caenorhabditis elegans Y53C10A.2 (Y53C10A.2) mRNA, complete cds. 11/11 None
SNAP00000000680 ENSEMBL cdna:SNAP chromosome:CEL140:I:11961925:11976782:-1 11/11 None
GENEFINDER00000000695 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:11976144:11976665:-1 11/11 None
Y53C10A.2 ENSEMBL cdna:known chromosome:CEL140:I:11976144:11976665:-1 gene:Y53C10A.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:11976074-11976596(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y53C10A.2
Entrez Gene 190191 Entrez gene
SwissProt Q9XW55 EMBL-EBI
WormBase CE22370 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAS72409 heat shock transcription factor 1 [Caenorhabditis elegans] emb|CAA22146.1| Hypothetical protein Y53C10A.12 [Caenorhabditis elegans] ref|NP_493031.1| Heat Shock Factor family member (hsf-1) [Caenorhabditis elegans] 0.0
blast CAE71722 Hypothetical protein CBG18699 [Caenorhabditis briggsae] 1.0E-154
blast CAA22136 Hypothetical protein Y53C10A.2 [Caenorhabditis elegans] ref|NP_493033.1| Y53C10A.2 [Caenorhabditis elegans] 2.0E-70
blast CAA22136 Hypothetical protein Y53C10A.2 [Caenorhabditis elegans] ref|NP_493033.1| Y53C10A.2 [Caenorhabditis elegans] 2.0E-64
Protein Domains
Database ID Description E-Value
scop a.2.7.Seryl-tRNA synthetase (SerRS) All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Seryl-tRNA synthetase (SerRS) 1.10000002384186
Pfam IPR000232 EMBL-EBI Heat shock factor (HSF)-type DNA-binding domain 7.8E-60
Sequence
Target Sequence 
>C. ELEGANS:184473_AT
atatcaagagatcgtcggctcctgcggctcctgctcctgtgacagtgttgaaaatgtggc
caaacgaaatgagaagccagtcgttggaagagaatcttgacgcaaaacttgagcaattca
aaaaggagcattctccggtcaaggaacgaggaaattgctccaaaacacgaaaaatatccg
agaatcagccgccgccgagctttgaagttgtcatggctaagattcgtggaattcaagaaa
gtttgaagaatatggaagatcagatgaatgaagaagagcgcggaaaggaaatgccagatt
cgactgaaaattccttcgacttttcgaaaatttggcaatatttcgatcagacgaggatcg
aggaaattcgtgcgctttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATATCAAGAGATCGTCGGCTCCTGC 89 21 23 Antisense
CTCCTGCTCCTGTGACAGTGTTGAA 441 235 50 Antisense
GAATCTTGACGCAAAACTTGAGCAA 152 329 114 Antisense
GAGCATTCTCCGGTCAAGGAACGAG 549 387 148 Antisense
AAAATATCCGAGAATCAGCCGCCGC 10 135 193 Antisense
GCCGCCGAGCTTTGAAGTTGTCATG 249 285 213 Antisense
AGTTGTCATGGCTAAGATTCGTGGA 582 63 228 Antisense
GAGCGCGGAAAGGAAATGCCAGATT 245 385 298 Antisense
GCCAGATTCGACTGAAAATTCCTTC 504 277 315 Antisense
ATTTGGCAATATTTCGATCAGACGA 54 19 352 Antisense
GGATCGAGGAAATTCGTGCGCTTTT 140 513 377 Antisense
Sequence Source Affymetrix Proprietary Database