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[184461_s_at]

Description K04F10.5 /REP_DB=WormBase Gene ID /WP=CE11738 /TR=O44767 /GB=AAB96752.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 04      4grp_total > 26      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 82
1 2 3 4 range
A 12.7
(-3)
A 		28.6
(-0.8)
A 		15.9
(-2.6)
A 		56.5
(3)
A 		44
B 18.1
(-1.5)
A 		43
(3)
A 		23.4
(-0.5)
A 		9.8
(-3)
A 		33
C 18.9
(-3)
A 		73.8
(1.5)
A 		24.8
(-2.5)
A 		91.6
(3)
A 		73
avr 16.6
(-3) 		48.5
(2.3) 		21.4
(-2.2) 		52.6
(3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 84
1 2 3 4 5 6 range
A 42.3
(2.2)
A 		47.8
(3)
A 		17.4
(-1.3)
A 		5.8
(-3)
A 		30.8
(0.6)
A 		26.7
(-0)
A 		42
B 60.9
(3)
A 		50.2
(1.5)
A 		53.7
(2)
A 		24.9
(-2.1)
A 		18.5
(-3)
A 		19.8
(-2.8)
A 		42
C 40.3
(-1)
A 		15.5
(-3)
A 		23.6
(-2.3)
A 		90.2
(3)
A 		54.9
(0.2)
A 		22.6
(-2.4)
A 		75
avr 47.8
(3) 		37.8
(0.6) 		31.6
(-0.9) 		40.3
(1.2) 		34.7
(-0.2) 		23
(-3) 		25
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 4.6
(-2.4)
A 		6.2
(-0.1)
A 		4.2
(-3)
A 		8.3
(3)
A 		4
B 5.8
(-0.4)
A 		10.6
(3)
A 		5.4
(-0.7)
A 		2.1
(-3)
A 		9
C 6.3
(-3)
A 		21.2
(3)
A 		8.7
(-2)
A 		15.5
(0.7)
A 		15
avr 5.6
(-3) 		12.7
(3) 		6.1
(-2.6) 		8.6
(-0.5) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 7.8
(-0.6)
A 		17
(3)
A 		3.8
(-2.1)
A 		1.5
(-3)
A 		9.3
(0)
A 		6.5
(-1.1)
A 		16
B 21.2
(3)
A 		16.7
(1.2)
A 		13
(-0.4)
A 		6.6
(-3)
A 		7.5
(-2.6)
A 		7.4
(-2.7)
A 		15
C 14.9
(1.6)
A 		4.7
(-3)
A 		6
(-2.4)
A 		18.1
(3)
A 		17.8
(2.9)
A 		7.6
(-1.7)
A 		13
avr 14.6
(3) 		12.8
(1.5) 		7.6
(-2.7) 		8.7
(-1.8) 		11.5
(0.5) 		7.2
(-3) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184461_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1872
Sequence Type Exemplar sequence
Representative Public ID K04F10.5 NCBI
Target Description K04F10.5 /REP_DB=WormBase Gene ID /WP=CE11738 /TR=O44767 /GB=AAB96752.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059432(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059432 NCBI Caenorhabditis elegans K04F10.5 (K04F10.5) mRNA, complete cds. 11/11 A 
SNAP00000038584 ENSEMBL cdna:SNAP chromosome:CEL140:II:9667211:9674735:-1 11/11 None
GENEFINDER00000038593 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:9673317:9674735:-1 11/11 None
K04F10.5 ENSEMBL cdna:known chromosome:CEL140:I:6358390:6360730:-1 gene:K04F10.5 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000027818 2/11 Cross Hyb Matching Probes None
GENEFINDER00000027831 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6358318-6360659(-) 100.0 100.0
Public Domain and Genome References
Ensembl K04F10.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_491833 K04F10.5 [Caenorhabditis elegans] 0.0
blast T27754 hypothetical protein ZK1320.8 - Caenorhabditis elegans 0.0
blast T19605 hypothetical protein C31C9.5 - Caenorhabditis elegans 1.0E-132
blast T19605 hypothetical protein C31C9.5 - Caenorhabditis elegans 1.0E-130
blast AAK39265 Hypothetical protein R11E3.3 [Caenorhabditis elegans] ref|NP_500640.1| R11E3.3 [Caenorhabditis elegans] 1.0E-130
blast AAK39265 Hypothetical protein R11E3.3 [Caenorhabditis elegans] ref|NP_500640.1| R11E3.3 [Caenorhabditis elegans] 1.0E-128
blast AAK39265 Hypothetical protein R11E3.3 [Caenorhabditis elegans] ref|NP_500640.1| R11E3.3 [Caenorhabditis elegans] 1.0E-112
Protein Domains
Database ID Description E-Value
Pfam IPR000477 EMBL-EBI RNA-directed DNA polymerase (Reverse transcriptase) 5.7E-18
Pfam IPR005135 EMBL-EBI Endonuclease/exonuclease/phosphatase 1.1E-27
Pfam IPR000477 EMBL-EBI RNA-directed DNA polymerase (Reverse transcriptase) 2.7E-31
Sequence
Target Sequence 
>C. ELEGANS:184461_S_AT
gaccaaaacccacttgagcagactcaaaggtacgaaggcagaagcgcaaaaggccaatca
cacaagattcgttcgcgaattcttgaactcggccccacccaacaggatcctgggagccac
gccgccaccattgaatagttctgaagaatctagcctcccaaaagccaccagaacggaact
ggctcgacttcgctgtggacactccttactgatccccaggtacaaagcgagaattgaagg
aacccccatcggacatgcggatgtggagacaacgagggagggatcgatcactacttatcc
tgtggaagatcgcatcccatatcgaaagaaaaactatggacgtccccaatggaggccgcc
cgagccctcgggctggcctacaaaccatatgacccgggtggaggagcaacgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACCAAAACCCACTTGAGCAGACTC 705 383 1740 Antisense
GTTCGCGAATTCTTGAACTCGGCCC 624 441 1810 Antisense
GCCGCCACCATTGAATAGTTCTGAA 348 285 1860 Antisense
AGTTCTGAAGAATCTAGCCTCCCAA 63 65 1876 Antisense
GCCACCAGAACGGAACTGGCTCGAC 317 279 1903 Antisense
TCCTTACTGATCCCCAGGTACAAAG 600 581 1942 Antisense
GATCGATCACTACTTATCCTGTGGA 693 417 2021 Antisense
GTGGAAGATCGCATCCCATATCGAA 558 491 2041 Antisense
AACTATGGACGTCCCCAATGGAGGC 489 137 2071 Antisense
GGCTGGCCTACAAACCATATGACCC 326 541 2110 Antisense
ATGACCCGGGTGGAGGAGCAACGAC 258 45 2128 Antisense
Sequence Source Affymetrix Proprietary Database