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[184460_at]

Description K04F10.5 /REP_DB=WormBase Gene ID /WP=CE11738 /TR=O44767 /GB=AAB96752.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 74      4grp_total > 43      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 220
1 2 3 4 range
A 63.7
(-3)
A 		106.3
(1.8)
A 		116.9
(3)
A 		96
(0.6)
P 		53
B 60.1
(3)
A 		9.3
(-3)
A 		47.8
(1.5)
A 		45.3
(1.3)
A 		51
C 60.9
(-2.5)
A 		229.7
(3)
M 		45.2
(-3)
A 		89.4
(-1.6)
P 		185
avr 61.6
(-3) 		115.1
(3) 		70
(-2.1) 		76.9
(-1.3) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 74
1 2 3 4 5 6 range
A 2
(-3)
A 		9.7
(-2.4)
A 		25.1
(-1.1)
A 		76.4
(3)
A 		22.9
(-1.3)
A 		57.7
(1.5)
A 		74
B 43.3
(0.3)
A 		33.2
(-0.6)
A 		40.1
(0)
A 		73.9
(3)
A 		5.8
(-3)
A 		28.1
(-1)
A 		68
C 17.2
(-3)
A 		18
(-2.9)
A 		58.9
(3)
A 		38.8
(0.1)
A 		49.2
(1.6)
A 		44.7
(1)
A 		42
avr 20.8
(-2.9) 		20.3
(-3) 		41.4
(-0) 		63
(3) 		26
(-2.2) 		43.5
(0.3) 		43
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 64
1 2 3 4 range
A 23.2
(0.3)
A 		23.1
(0.3)
A 		30.6
(3)
A 		14.1
(-3)
P 		17
B 19.2
(3)
A 		2.3
(-3)
A 		11.1
(0.1)
A 		9.7
(-0.4)
A 		17
C 20.2
(-2.4)
A 		66
(3)
M 		15.8
(-2.9)
A 		15.1
(-3)
P 		51
avr 20.9
(-0.3) 		30.5
(3) 		19.2
(-0.9) 		13
(-3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 0.4
(-3)
A 		3.4
(-2.1)
A 		5.5
(-1.4)
A 		19.4
(3)
A 		6.9
(-0.9)
A 		14.1
(1.3)
A 		19
B 15.1
(1.4)
A 		11.1
(0.1)
A 		9.7
(-0.4)
A 		19.6
(3)
A 		2.3
(-3)
A 		10.5
(-0.2)
A 		17
C 6.4
(-2.5)
A 		5.5
(-3)
A 		15
(2.5)
A 		7.8
(-1.7)
A 		15.9
(3)
A 		15
(2.5)
A 		10
avr 7.3
(-2.6) 		6.7
(-3) 		10.1
(-0.7) 		15.6
(3) 		8.4
(-1.9) 		13.2
(1.4) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184460_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1872
Sequence Type Exemplar sequence
Representative Public ID K04F10.5 NCBI
Target Description K04F10.5 /REP_DB=WormBase Gene ID /WP=CE11738 /TR=O44767 /GB=AAB96752.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059432(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059432 NCBI Caenorhabditis elegans K04F10.5 (K04F10.5) mRNA, complete cds. 11/11 A 
K04F10.5 ENSEMBL cdna:known chromosome:CEL140:I:6358390:6360730:-1 gene:K04F10.5 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000027818 4/11 Cross Hyb Matching Probes None
GENEFINDER00000027831 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6358318-6360659(-) 100.0 100.0
Public Domain and Genome References
Ensembl K04F10.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_491833 K04F10.5 [Caenorhabditis elegans] 0.0
blast AAK39265 Hypothetical protein R11E3.3 [Caenorhabditis elegans] ref|NP_500640.1| R11E3.3 [Caenorhabditis elegans] 1.0E-112
blast T27754 hypothetical protein ZK1320.8 - Caenorhabditis elegans 1.0E-32
Protein Domains
Database ID Description E-Value
Pfam IPR000477 EMBL-EBI RNA-directed DNA polymerase (Reverse transcriptase) 5.7E-18
Pfam IPR005135 EMBL-EBI Endonuclease/exonuclease/phosphatase 1.1E-27
Sequence
Target Sequence 
>C. ELEGANS:184460_AT
acgtcaactgcactcactgaggtcatcaactccatcgtcggcggactcaacatgaagcag
ccggcaaagcgcacagaggcgaccaaaacccacttgagcagactcaaaggtacgaaggca
gaagcgcaaaaggccaatcacacaagattcgttcgcgaattcttgaactcggccccaccc
aacaggatcctgggagccacgccgccaccattgaatagttctgaagaatctagcctccca
aaagccaccagaacggaactggctcgacttcgctgtggacactccttactgatccccagg
tacaaagcgagaattgaaggaacccccatcggacatgcggatgtggagacaacgagggag
ggatcgatcactacttatcctgtggaagatcgcatcccatatcgaaagaaaaactatgga
cgtccccaatggaggccgcccgagccctcgggctggcctacaaaccatatgacccgggtg
gaggagcaacgacgagcgtctgaaaacggcgctgcgccgcctgttgcacaacaacaacaa
agatttattatgcatcaccgtctccaaattaaatta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGTCAACTGCACTCACTGAGGTCA 626 93 1660 Antisense
CTCACTGAGGTCATCAACTCCATCG 273 227 1672 Antisense
TCCATCGTCGGCGGACTCAACATGA 120 591 1690 Antisense
GCGGACTCAACATGAAGCAGCCGGC 567 293 1700 Antisense
CGGCAAAGCGCACAGAGGCGACCAA 633 241 1721 Antisense
AAAGCGCACAGAGGCGACCAAAACC 324 125 1725 Antisense
GACGAGCGTCTGAAAACGGCGCTGC 424 379 2150 Antisense
TGAAAACGGCGCTGCGCCGCCTGTT 413 571 2160 Antisense
AGATTTATTATGCATCACCGTCTCC 228 65 2200 Antisense
TATGCATCACCGTCTCCAAATTAAA 420 657 2208 Antisense
GCATCACCGTCTCCAAATTAAATTA 166 307 2211 Antisense
Sequence Source Affymetrix Proprietary Database