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[184454_at]

Description Y59A8B.23 /REP_DB=WormBase Gene ID /WP=CE26212 /TR=Q9GRY2 /GB=CAC14417.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 12      4grp_highest_8_8 > 39      4grp_high_8_8 > 12      6grp_highest_nonorm > 80      6grp_total > 23      4grp_total > 12      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 144
1 2 3 4 range
A 111.5
(-1.8)
P 		119
(-1.5)
P 		86.8
(-3)
P 		214
(3)
P 		127
B 138.9
(3)
P 		105.7
(0.1)
P 		69.6
(-3)
P 		137.4
(2.9)
P 		69
C 122.7
(-1)
P 		105.8
(-2)
P 		90
(-3)
P 		187.8
(3)
P 		98
avr 124.4
(-0.4) 		110.2
(-1.3) 		82.1
(-3) 		179.7
(3) 		98

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 269
1 2 3 4 5 6 range
A 363.3
(3)
P 		102
(-2.8)
P 		267.9
(0.9)
P 		136.6
(-2.1)
P 		238.5
(0.2)
P 		94.5
(-3)
P 		269
B 363
(3)
P 		339.1
(2.4)
P 		306.6
(1.6)
P 		214.1
(-0.8)
P 		128.1
(-3)
P 		146.8
(-2.5)
P 		235
C 126.4
(-2.1)
P 		274.4
(3)
P 		119.8
(-2.4)
P 		236.9
(1.7)
P 		101.2
(-3)
P 		138.4
(-1.7)
P 		173
avr 284.2
(3) 		238.5
(1.3) 		231.4
(1) 		195.9
(-0.4) 		155.9
(-1.9) 		126.6
(-3) 		158
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 28
1 2 3 4 range
A 40.6
(3)
P 		25.8
(-2)
P 		22.7
(-3)
P 		31.3
(-0.1)
P 		18
B 44.3
(3)
P 		26.1
(-0.9)
P 		16.1
(-3)
P 		29.3
(-0.2)
P 		28
C 40.6
(3)
P 		30.4
(-3)
P 		31.4
(-2.4)
P 		31.7
(-2.2)
P 		10
avr 41.8
(3) 		27.4
(-1.7) 		23.4
(-3) 		30.8
(-0.6) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 103
1 2 3 4 5 6 range
A 67
(2.4)
P 		36.3
(-1.4)
P 		58.3
(1.3)
P 		34.6
(-1.6)
P 		72
(3)
P 		23
(-3)
P 		49
B 126.3
(3)
P 		112.9
(1.9)
P 		74.4
(-1.2)
P 		56.7
(-2.6)
P 		52
(-3)
P 		54.7
(-2.8)
P 		74
C 46.8
(-1.2)
P 		83.7
(3)
P 		30.5
(-3)
P 		47.6
(-1.1)
P 		32.8
(-2.7)
P 		46.3
(-1.2)
P 		53
avr 80
(3) 		77.6
(2.6) 		54.4
(-1) 		46.3
(-2.2) 		52.3
(-1.3) 		41.3
(-3) 		39
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184454_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13901
Sequence Type Exemplar sequence
Representative Public ID Y59A8B.23 NCBI
Target Description Y59A8B.23 /REP_DB=WormBase Gene ID /WP=CE26212 /TR=Q9GRY2 /GB=CAC14417.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075116(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075116 NCBI Caenorhabditis elegans Y59A8B.23 (Y59A8B.23) mRNA, complete cds. 11/11 None
GENEFINDER00000028719 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:17956697:17966421:-1 11/11 None
Y59A8B.23 ENSEMBL cdna:known chromosome:CEL140:V:17956309:17969423:-1 gene:Y59A8B.23 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000028692 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:17956696-17967928(-) 98.72 100.0
Public Domain and Genome References
Gene Title
Ensembl Y59A8B.23
Entrez Gene 190400 Entrez gene
SwissProt Q9GRY2 EMBL-EBI
WormBase CE38123 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:246583_AT protein kinase family protein at
CANINE_2:CFAAFFX.8385.1.S1_AT similar to oxidative-stress responsive 1 cfa
CANINE_2:CFAAFFX.8390.1.S1_AT similar to oxidative-stress responsive 1 cfa
CANINE_2:CFAAFFX.8390.1.S1_S_AT similar to oxidative-stress responsive 1 cfa
DROSOPHILA_2:1626099_S_AT frayed dm
DROSGENOME1:144108_AT frayed dm
CHICKEN:GGA.3394.1.S1_AT oxidative-stress responsive 1 gga
HG-U133_PLUS_2:202696_AT oxidative-stress responsive 1 hs
HG-U133A:202696_AT oxidative-stress responsive 1 hs
HG-FOCUS:202696_AT oxidative-stress responsive 1 hs
HG-U133A_2:202696_AT oxidative-stress responsive 1 hs
HU35KSUBC:RC_H61542_S_AT oxidative-stress responsive 1 hs
HU35KSUBC:RC_AA039663_AT oxidative-stress responsive 1 hs
U133_X3P:G4826877_3P_AT oxidative-stress responsive 1 hs
HG-U95AV2:39136_AT oxidative-stress responsive 1 hs
HG-U95E:82724_AT Oxidative-stress responsive 1 hs
MG-U74BV2:114314_AT oxidative-stress responsive 1 mm
MG-U74BV2:164672_F_AT oxidative-stress responsive 1 mm
MG-U74CV2:166696_AT oxidative-stress responsive 1 mm
MOE430A:1426933_AT oxidative-stress responsive 1 mm
MOUSE430A_2:1426933_AT oxidative-stress responsive 1 mm
MOUSE430_2:1426933_AT oxidative-stress responsive 1 mm
MU19KSUBA:TC18856_AT Oxidative-stress responsive 1, mRNA (cDNA clone IMAGE:3711265) mm
RG-U34A:RC_AA875244_AT oxidative-stress responsive 1 (predicted) rn
RG-U34B:RC_AI059517_AT oxidative-stress responsive 1 (predicted) rn
RAE230A:1372096_AT Oxidative-stress responsive 1 (predicted) rn
RAT230_2:1372096_AT Oxidative-stress responsive 1 (predicted) rn
RAE230B:1380687_AT Oxidative-stress responsive 1 (predicted) rn
RAT230_2:1380687_AT Oxidative-stress responsive 1 (predicted) rn
RG-U34C:RC_AI178912_AT Oxidative-stress responsive 1 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAC14417 Hypothetical protein Y59A8B.23 [Caenorhabditis elegans] gb|AAU89102.1| STE20-like serine/threonine kinase [Caenorhabditis elegans] ref|NP_507517.2| Y59A8B.23 [Caenorhabditis elegans] 0.0
blast CAE61696 Hypothetical protein CBG05644 [Caenorhabditis briggsae] 0.0
blast XP_396480 PREDICTED: similar to Ste20-like protein kinase [Apis mellifera] 1.0E-145
blast XP_396480 PREDICTED: similar to Ste20-like protein kinase [Apis mellifera] 1.0E-121
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 8.0E-63
hanks 2.1.5 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; DmCamKII 2.0E-66
hanks 1.9.6 AGC group; AGC Other; SPK1 2.0E-66
hanks 1.1.1 AGC group; AGC I Cyclic nucleotide regulated protein kinase (PKA & PKG); cAPKa 1.0E-18
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.3E-66
Pfam IPR000719 EMBL-EBI Protein kinase 0.061
Pfam IPR000719 EMBL-EBI Protein kinase 2.0E-46
Sequence
Target Sequence 
>C. ELEGANS:184454_AT
atatctggtacatactctcattgagaatctcgcttcagttccagtagtcgcccatcactc
gtcgaaaaaagtggcttctggaaagttgcgtaaagatgctcacggaaattgggaatttga
atacgactcaccacaagaaagtgatgatgatagtgatttggaggatgaggaacgtgaaaa
gaagaagaagaaggctagtgcttcagcatcaggagctggagcagctggagctgctggagg
agccactggaggagcagcaagtggtgctccaagtgctcaggaaggtggaggagccaccac
tccgtgtccagagacgttgaatatggtgctcagagttcggaatcaacagcgagagctcaa
tgatatcaagtttgactatacaaagtcggctgatactgttgaaggaattgctcacgaact
agtgaccgctgaactcatcgactgtcacgatttggtaattgtcgctgccaaccttcagaa
gcttatcgattttgctgaatcaaagtctgatagacggtcgataacgtttgcattgaattc
tggagtacacgctaatgagatccccgacgagcggacattgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATATCTGGTACATACTCTCATTGAG 327 21 1038 Antisense
TTGAGAATCTCGCTTCAGTTCCAGT 428 701 1058 Antisense
GGCTAGTGCTTCAGCATCAGGAGCT 180 537 1230 Antisense
GCAGCAAGTGGTGCTCCAAGTGCTC 288 313 1291 Antisense
CCACTCCGTGTCCAGAGACGTTGAA 272 275 1334 Antisense
GGTGCTCAGAGTTCGGAATCAACAG 612 495 1362 Antisense
ATTGCTCACGAACTAGTGACCGCTG 400 5 1444 Antisense
TGACCGCTGAACTCATCGACTGTCA 216 569 1460 Antisense
TTGGTAATTGTCGCTGCCAACCTTC 534 701 1489 Antisense
AGTACACGCTAATGAGATCCCCGAC 707 61 1581 Antisense
AGATCCCCGACGAGCGGACATTGAC 426 67 1595 Antisense
Sequence Source Affymetrix Proprietary Database