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[184449_at]

Description Y56A3A.21 /REP_DB=WormBase Gene ID /WP=CE22589 /TR=Q9U238 /GB=CAB60502.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 02      6grp_high1_10_10 > 01      4grp_highest_8_8 > 10      4grp_high_8_8 > 54      6grp_highest_nonorm > 14      6grp_total > 23      4grp_total > 57      6grp_high1_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4598
1 2 3 4 range
A 3261
(-2.2)
P 		5866.3
(1.8)
P 		6660.4
(3)
P 		2728.5
(-3)
P 		3932
B 3588.5
(-1.7)
P 		5691.6
(1.3)
P 		6859.1
(3)
P 		2726.4
(-3)
P 		4133
C 3385.9
(-1.4)
P 		5973.7
(2.3)
P 		6503.6
(3)
P 		2261.5
(-3)
P 		4242
avr 3411.8
(-1.8) 		5843.9
(1.8) 		6674.4
(3) 		2572.1
(-3) 		4102

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1910
1 2 3 4 5 6 range
A 4234.5
(3)
P 		3330.4
(-1.4)
P 		4005.9
(1.9)
P 		3546.5
(-0.3)
P 		3490.3
(-0.6)
P 		2991.3
(-3)
P 		1243
B 4249.5
(3)
P 		3748.7
(1.1)
P 		4157.2
(2.7)
P 		3859.1
(1.6)
P 		3481.6
(0.2)
P 		2625.7
(-3)
P 		1624
C 4334.5
(3)
P 		3519.1
(0.4)
P 		3745.5
(1.1)
P 		3764.2
(1.2)
P 		3322.8
(-0.2)
P 		2424.8
(-3)
P 		1910
avr 4272.8
(3) 		3532.7
(0.2) 		3969.5
(1.9) 		3723.3
(0.9) 		3431.6
(-0.2) 		2680.6
(-3) 		1592
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1885
1 2 3 4 range
A 1187.3
(0.5)
P 		1273.4
(0.9)
P 		1741.2
(3)
P 		399.4
(-3)
P 		1342
B 1144.3
(0.4)
P 		1405.7
(1.9)
P 		1589.4
(3)
P 		581
(-3)
P 		1008
C 1120.8
(-0.6)
P 		1717.5
(1.3)
P 		2266.7
(3)
P 		381.3
(-3)
P 		1885
avr 1150.8
(-0) 		1465.5
(1.3) 		1865.8
(3) 		453.9
(-3) 		1412

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 878
1 2 3 4 5 6 range
A 780.6
(-2.3)
P 		1184.4
(3)
P 		872.4
(-1.1)
P 		899.5
(-0.7)
P 		1053.2
(1.3)
P 		728.3
(-3)
P 		456
B 1478.6
(3)
P 		1247.8
(0.2)
P 		1009.5
(-2.6)
P 		1022.2
(-2.5)
P 		1414.5
(2.2)
P 		979.1
(-3)
P 		500
C 1606.6
(3)
P 		1073.8
(-0.8)
P 		953.3
(-1.6)
P 		757
(-3)
P 		1075.2
(-0.8)
P 		811.7
(-2.6)
P 		850
avr 1288.6
(3) 		1168.7
(1.4) 		945.1
(-1.6) 		892.9
(-2.3) 		1181
(1.6) 		839.7
(-3) 		449
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184449_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5997
Sequence Type Exemplar sequence
Representative Public ID Y56A3A.21 NCBI
Target Description Y56A3A.21 /REP_DB=WormBase Gene ID /WP=CE22589 /TR=Q9U238 /GB=CAB60502.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067153(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067153 NCBI Caenorhabditis elegans Y56A3A.21 (Y56A3A.21) mRNA, complete cds. 11/11 None
SNAP00000035200 ENSEMBL cdna:SNAP chromosome:CEL140:III:11920617:11921282:1 11/11 None
GENEFINDER00000035228 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:11920617:11921282:1 11/11 None
Y56A3A.21.2 ENSEMBL cdna:known chromosome:CEL140:III:11920592:11921493:1 gene:Y56A3A.21 11/11 None
Y56A3A.21.1 ENSEMBL cdna:known chromosome:CEL140:III:11920611:11921497:1 gene:Y56A3A.21 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:11920612-11921278(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y56A3A.21
Entrez Gene 176626 Entrez gene
SwissProt Q9U238 EMBL-EBI
WormBase CE22589 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5783 endoplasmic reticulum inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB60502 Hypothetical protein Y56A3A.21 [Caenorhabditis elegans] ref|NP_499554.1| Y56A3A.21 [Caenorhabditis elegans] 6.0E-78
blast CAE67746 Hypothetical protein CBG13321 [Caenorhabditis briggsae] emb|CAE67741.1| Hypothetical protein CBG13316 [Caenorhabditis briggsae] 4.0E-67
Protein Domains
Database ID Description E-Value
Pfam IPR008855 EMBL-EBI Translocon-associated 2.0E-108
Sequence
Target Sequence 
>C. ELEGANS:184449_AT
cgagtcgccaaagtactcggcctcgtcattctccactaccgacggattcttccactacaa
aaccaccttcatcaccgagttcacacttcaatgcagcaacaacccgaagaatatccaata
cactgctgaagtaaacggtcgccttatcccagtctcgatttccgacgagaccgccaagtt
ccaagtctcatggactcttgagcacaaggacgccggagctcaaacctttaatattaacat
tttcgacgaggagggagccgctcaatacgccaagaaccctgtcaccaagccactcttcac
cgttcaacaaagccatgggggtctcgccacgaagtctccaatctcttccgagactgtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGAGTCGCCAAAGTACTCGGCCTCG 336 249 69 Antisense
TCATCACCGAGTTCACACTTCAATG 577 619 137 Antisense
GCTGAAGTAAACGGTCGCCTTATCC 423 293 193 Antisense
TATCCCAGTCTCGATTTCCGACGAG 630 655 213 Antisense
GAGACCGCCAAGTTCCAAGTCTCAT 114 393 235 Antisense
CAAGTCTCATGGACTCTTGAGCACA 209 185 250 Antisense
CGCCGGAGCTCAAACCTTTAATATT 575 255 279 Antisense
CGAGGAGGGAGCCGCTCAATACGCC 68 251 315 Antisense
CACTCTTCACCGTTCAACAAAGCCA 368 197 359 Antisense
TCAACAAAGCCATGGGGGTCTCGCC 170 627 372 Antisense
GTCTCCAATCTCTTCCGAGACTGTC 229 469 402 Antisense
Sequence Source Affymetrix Proprietary Database