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[184448_at]

Description F41G3.10 /REP_DB=WormBase Gene ID /WP=CE01283 /TR=Q20289 /GB=AAA81107.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 13      6grp_high1_10_10 > 21      4grp_highest_8_8 > 48      4grp_high_8_8 > 42      6grp_highest_nonorm > 14      6grp_total > 32      4grp_total > 53      6grp_high1_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6221
1 2 3 4 range
A 5943
(3)
P 		4256.3
(1.2)
P 		3220.8
(0.1)
P 		370.1
(-3)
P 		5573
B 6543.1
(3)
P 		3866.6
(0.4)
P 		2850.4
(-0.6)
P 		354.8
(-3)
P 		6188
C 6008.9
(3)
P 		3894.8
(0.8)
P 		2791.2
(-0.4)
P 		322.6
(-3)
P 		5686
avr 6165
(3) 		4005.9
(0.8) 		2954.1
(-0.3) 		349.2
(-3) 		5816

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5109
1 2 3 4 5 6 range
A 5077.1
(2.7)
P 		4335.7
(1.7)
P 		5332.2
(3)
P 		3923.3
(1.1)
P 		2046
(-1.3)
P 		797.8
(-3)
P 		4534
B 4850.7
(1.7)
P 		4318.5
(1.1)
P 		5907.2
(3)
P 		3596.3
(0.2)
P 		2167.5
(-1.6)
P 		1018.1
(-3)
P 		4889
C 4689.6
(2.5)
P 		4163.6
(1.7)
P 		5033.1
(3)
P 		3670.7
(1)
P 		1950.9
(-1.6)
P 		1034.3
(-3)
P 		3999
avr 4872.5
(2.3) 		4272.6
(1.5) 		5424.2
(3) 		3730.1
(0.7) 		2054.8
(-1.5) 		950.1
(-3) 		4474
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2110
1 2 3 4 range
A 2163.8
(3)
P 		923.9
(-0.5)
P 		842
(-0.8)
P 		54.2
(-3)
P 		2110
B 2086.5
(3)
P 		955
(-0.4)
P 		660.5
(-1.3)
P 		75.6
(-3)
P 		2011
C 1989.1
(3)
P 		1119.8
(0.3)
P 		972.8
(-0.2)
P 		54.4
(-3)
P 		1935
avr 2079.8
(3) 		999.6
(-0.2) 		825.1
(-0.7) 		61.4
(-3) 		2018

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1544
1 2 3 4 5 6 range
A 935.9
(0.3)
P 		1542
(3)
P 		1161.2
(1.3)
P 		995
(0.6)
P 		617.4
(-1.1)
P 		194.3
(-3)
P 		1348
B 1687.8
(3)
P 		1437.4
(1.9)
P 		1434.5
(1.8)
P 		952.5
(-0.4)
P 		880.6
(-0.7)
P 		379.7
(-3)
P 		1308
C 1738.2
(3)
P 		1270.4
(1)
P 		1281
(1)
P 		738.2
(-1.3)
P 		631.3
(-1.8)
P 		346.2
(-3)
P 		1392
avr 1454
(3) 		1416.6
(2.8) 		1292.2
(2.2) 		895.2
(0.1) 		709.8
(-0.9) 		306.7
(-3) 		1147
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184448_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5986
Sequence Type Exemplar sequence
Representative Public ID F41G3.10 NCBI
Target Description F41G3.10 /REP_DB=WormBase Gene ID /WP=CE01283 /TR=Q20289 /GB=AAA81107.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026986, NM_062978
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062978 NCBI Caenorhabditis elegans F41G3.10 (F41G3.10) mRNA, complete cds. None
SNAP00000017943 ENSEMBL cdna:SNAP chromosome:CEL140:II:6748626:6749450:-1 None
GENEFINDER00000017959 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6748626:6752061:-1 None
SNAP00000017944 ENSEMBL cdna:SNAP chromosome:CEL140:II:6749801:6750946:-1 None
F41G3.10 ENSEMBL cdna:known chromosome:CEL140:II:6748566:6749457:-1 gene:F41G3.10 None
NM_001026986 NCBI Caenorhabditis elegans F41G3.20 (F41G3.20) mRNA, complete cds. None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6748621-6752417(-) 100.0 100.0
Public Domain and Genome References
Gene Title

Ensembl F41G3.10
Entrez Gene 185632 Entrez gene
3565874 Entrez gene
SwissProt Q20289 EMBL-EBI
WormBase CE31937 Wormbase
CE38601 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAY44012 Hypothetical protein F41G3.20 [Caenorhabditis elegans] ref|NP_001022157.1| F41G3.20 [Caenorhabditis elegans] 1.0E-112
blast CAE65957 Hypothetical protein CBG11139 [Caenorhabditis briggsae] 8.0E-81
blast CAE65957 Hypothetical protein CBG11139 [Caenorhabditis briggsae] 3.0E-80
blast CAE65956 Hypothetical protein CBG11138 [Caenorhabditis briggsae] 1.0E-77
blast CAE65956 Hypothetical protein CBG11138 [Caenorhabditis briggsae] 2.0E-76
blast AAA81107 Hypothetical protein F41G3.10 [Caenorhabditis elegans] ref|NP_495379.2| F41G3.10 [Caenorhabditis elegans] 1.0E-75
blast AAA81107 Hypothetical protein F41G3.10 [Caenorhabditis elegans] ref|NP_495379.2| F41G3.10 [Caenorhabditis elegans] 3.0E-67
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.8E-15
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.0E-15
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.8E-15
Pfam IPR007026 EMBL-EBI CC domain 1.8E-10
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 9.7E-16
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 9.1E-16
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.4E-15
Pfam IPR007026 EMBL-EBI CC domain 2.2E-9
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.1E-15
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 5.8E-15
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 9.7E-16
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.5E-15
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.8E-15
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.0E-15
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.8E-15
Pfam IPR007026 EMBL-EBI CC domain 1.8E-10
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.8E-15
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.0E-15
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.8E-15
Sequence
Target Sequence 
>C. ELEGANS:184448_AT
cttcatactgtaccaactctgcatatttgacacttatgaagcaacaatgtccaaaaacct
gtggatattgtaaagtaataatgctgttccaacttctgattgttgctgcttctatcggga
tttcggttcaacaactaggatgcaactccgtaccaatgccggatcttaatggagcttgcc
caccaggaaccacactcattgctggaagtggttgttgccctgatggagacgttttcacaa
tcaccacaacatctacagcagcagctgctgtccacaaccaccagaacttcagtactggaa
ctgctaccacaaccagaacctcaactacttgtgtcgattt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTCATACTGTACCAACTCTGCATA 282 221 392 Antisense
ACTTCTGATTGTTGCTGCTTCTATC 525 103 483 Antisense
GCTTCTATCGGGATTTCGGTTCAAC 8 303 499 Antisense
GCAACTCCGTACCAATGCCGGATCT 578 319 533 Antisense
GGATCTTAATGGAGCTTGCCCACCA 658 511 552 Antisense
GAAGTGGTTGTTGCCCTGATGGAGA 490 335 596 Antisense
GAGACGTTTTCACAATCACCACAAC 117 393 617 Antisense
ACCACAACATCTACAGCAGCAGCTG 422 85 634 Antisense
TCCACAACCACCAGAACTTCAGTAC 516 587 662 Antisense
AGTACTGGAACTGCTACCACAACCA 171 63 682 Antisense
GAACCTCAACTACTTGTGTCGATTT 360 345 707 Antisense
Sequence Source Affymetrix Proprietary Database