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[184446_at]

Description Y50C1A.2 /REP_DB=WormBase Gene ID /WP=CE24398 /TR=Q9N4T4 /GB=AAF60742.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 67      4grp_total > 22      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 157
1 2 3 4 range
A 96.6
(0.4)
A 		24.4
(-2.3)
A 		6.5
(-3)
A 		163.5
(3)
A 		157
B 123.2
(3)
A 		88.5
(0.3)
A 		45
(-3)
A 		88.6
(0.3)
A 		78
C 117
(2.5)
A 		47.3
(-2.4)
A 		38.5
(-3)
A 		123.4
(3)
A 		85
avr 112.3
(2.2) 		53.4
(-1.5) 		30
(-3) 		125.2
(3) 		95

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 113
1 2 3 4 5 6 range
A 52
(-1.7)
A 		30.8
(-3)
A 		99.3
(1.2)
A 		63.2
(-1)
A 		30.1
(-3)
A 		128.7
(3)
A 		99
B 68.7
(-0.3)
A 		62.9
(-0.7)
A 		55.2
(-1.1)
A 		23
(-3)
A 		60.2
(-0.8)
A 		125.7
(3)
P 		103
C 78.8
(0.4)
A 		15.8
(-3)
A 		52.9
(-1)
A 		113.2
(2.3)
A 		52.2
(-1)
A 		125.6
(3)
M 		110
avr 66.5
(-1) 		36.5
(-3) 		69.1
(-0.8) 		66.5
(-1) 		47.5
(-2.3) 		126.7
(3) 		90
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 38
1 2 3 4 range
A 35.2
(3)
A 		5.3
(-2.4)
A 		1.7
(-3)
A 		23.9
(1)
A 		34
B 39.3
(3)
A 		21.9
(-0.6)
A 		10.4
(-3)
A 		18.9
(-1.2)
A 		29
C 38.7
(3)
A 		13.6
(-3)
A 		13.4
(-3)
A 		20.8
(-1.2)
A 		25
avr 37.7
(3) 		13.6
(-2) 		8.5
(-3) 		21.2
(-0.4) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 42
1 2 3 4 5 6 range
A 9.6
(-2.9)
A 		11
(-2.5)
A 		21.6
(0.4)
A 		16
(-1.1)
A 		9.1
(-3)
A 		31.3
(3)
A 		22
B 23.9
(-0.4)
A 		20.9
(-0.8)
A 		13.4
(-1.9)
A 		6.1
(-3)
A 		24.4
(-0.3)
A 		46.9
(3)
P 		41
C 29.2
(0.9)
A 		4.8
(-3)
A 		13.5
(-1.6)
A 		22.8
(-0.1)
A 		16.9
(-1.1)
A 		42.1
(3)
M 		37
avr 20.9
(-1.1) 		12.2
(-3) 		16.2
(-2.1) 		15
(-2.4) 		16.8
(-2) 		40.1
(3) 		28
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184446_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2563
Sequence Type Exemplar sequence
Representative Public ID Y50C1A.2 NCBI
Target Description Y50C1A.2 /REP_DB=WormBase Gene ID /WP=CE24398 /TR=Q9N4T4 /GB=AAF60742.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058330(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058330 NCBI Caenorhabditis elegans Y50C1A.2 (Y50C1A.2) mRNA, complete cds. 11/11 None
GENEFINDER00000037023 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:413591:418976:-1 11/11 None
SNAP00000037021 ENSEMBL cdna:SNAP chromosome:CEL140:I:415820:418663:-1 10/11 None
Y50C1A.2 ENSEMBL cdna:known chromosome:CEL140:I:415850:418663:-1 gene:Y50C1A.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:415873-418663(-) 85.01 85.01
Public Domain and Genome References
Gene Title
Ensembl Y50C1A.2
Entrez Gene 190091 Entrez gene
SwissProt Q9N4T4 EMBL-EBI
WormBase CE36749 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF60742 Hypothetical protein Y50C1A.2 [Caenorhabditis elegans] ref|NP_490731.2| Y50C1A.2 [Caenorhabditis elegans] 0.0
blast AAF60742 Hypothetical protein Y50C1A.2 [Caenorhabditis elegans] ref|NP_490731.2| Y50C1A.2 [Caenorhabditis elegans] 1.0E-149
blast CAE74338 Hypothetical protein CBG22052 [Caenorhabditis briggsae] 1.0E-142
blast CAE74338 Hypothetical protein CBG22052 [Caenorhabditis briggsae] 1.0E-141
blast CAE74338 Hypothetical protein CBG22052 [Caenorhabditis briggsae] 1.0E-117
Sequence
Target Sequence 
>C. ELEGANS:184446_AT
aaatgtgtcgtcacgggctcttatcaggctccgacgatggtaatataattttatggcaaa
tcggaacatcaggactcgaaaaactcggcgggacaaagctcacagtgtcggatctgtcgc
gaaaaattcgaagatcttcgacttctaataagcctgtcgcaattgttagcatgcaagtgt
acgtgtggccaagcggcgaagaagcgtgtgtggcaaccgagacgggtggtctttacctat
taactctaccaacattggactacaaaccgctcagccaccaaactgccacgtcaatcaata
aaatattatttgaaaatcaattcgtggcagtcatttatcatacgtcaaatgctgcagtat
tcaattctgaaggacttgtcgatgagattccatttgttgccacgtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAATGTGTCGTCACGGGCTCTTATC 68 121 635 Antisense
CTCTTATCAGGCTCCGACGATGGTA 373 227 652 Antisense
AGCTCACAGTGTCGGATCTGTCGCG 1 81 731 Antisense
TAAGCCTGTCGCAATTGTTAGCATG 478 637 783 Antisense
GCAAGTGTACGTGTGGCCAAGCGGC 524 319 807 Antisense
GAAGCGTGTGTGGCAACCGAGACGG 229 343 835 Antisense
TTTACCTATTAACTCTACCAACATT 79 665 866 Antisense
CATTGGACTACAAACCGCTCAGCCA 452 215 887 Antisense
CACCAAACTGCCACGTCAATCAATA 487 201 910 Antisense
GTGGCAGTCATTTATCATACGTCAA 597 489 958 Antisense
ATGAGATTCCATTTGTTGCCACGTT 251 45 1016 Antisense
Sequence Source Affymetrix Proprietary Database