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[184443_at]

Description Y45F10D.2 /REP_DB=WormBase Gene ID /WP=CE16641 /TR=O45945 /GB=CAA16377.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 62      6grp_high1_10_10 > 27      4grp_highest_8_8 > 31      4grp_high_8_8 > 13      6grp_highest_nonorm > 70      6grp_total > 26      4grp_total > 11      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 331
1 2 3 4 range
A 154.9
(-3)
P 		205.3
(-1.6)
P 		165.4
(-2.7)
P 		378.4
(3)
P 		224
B 215
(-2)
P 		176.9
(-2.8)
P 		165.3
(-3)
P 		451.5
(3)
P 		286
C 194.4
(-1.5)
P 		189.3
(-1.6)
P 		120.1
(-3)
P 		409.9
(3)
P 		290
avr 188.1
(-2.1) 		190.5
(-2.1) 		150.3
(-3) 		413.3
(3) 		263

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 538
1 2 3 4 5 6 range
A 532.3
(3)
P 		386.4
(0.9)
P 		186.9
(-2.1)
P 		140.1
(-2.7)
P 		122.5
(-3)
P 		309.5
(-0.3)
P 		410
B 469.7
(3)
P 		405.7
(1.9)
P 		221.3
(-1.1)
P 		119.8
(-2.8)
P 		107.9
(-3)
P 		197
(-1.5)
P 		362
C 645.9
(3)
P 		303.3
(-0.9)
P 		301.2
(-0.9)
P 		133.3
(-2.8)
P 		117.8
(-3)
P 		188.5
(-2.2)
P 		528
avr 549.3
(3) 		365.1
(0.4) 		236.5
(-1.3) 		131.1
(-2.8) 		116.1
(-3) 		231.7
(-1.4) 		433
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 58
1 2 3 4 range
A 56.4
(3)
P 		44.6
(-2.4)
P 		43.2
(-3)
P 		55.4
(2.5)
P 		13
B 68.6
(0.1)
P 		43.7
(-2.4)
P 		38.3
(-3)
P 		96.2
(3)
P 		58
C 64.3
(1.9)
P 		54.4
(-0.2)
P 		41.9
(-3)
P 		69.1
(3)
P 		27
avr 63.1
(1.1) 		47.6
(-1.8) 		41.1
(-3) 		73.6
(3) 		33

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 213
1 2 3 4 5 6 range
A 98.1
(0.7)
P 		137.4
(3)
P 		40.7
(-2.7)
P 		35.5
(-3)
P 		37
(-2.9)
P 		75.4
(-0.7)
P 		102
B 163.4
(3)
P 		135
(1.7)
P 		53.7
(-2)
P 		31.7
(-3)
P 		43.8
(-2.4)
P 		73.5
(-1.1)
P 		132
C 239.4
(3)
P 		92.5
(-1.1)
P 		76.7
(-1.6)
P 		26.8
(-3)
P 		38.1
(-2.7)
P 		63.1
(-2)
P 		213
avr 167
(3) 		121.6
(1) 		57
(-1.9) 		31.3
(-3) 		39.6
(-2.6) 		70.7
(-1.3) 		136
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184443_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9818
Sequence Type Exemplar sequence
Representative Public ID Y45F10D.2 NCBI
Target Description Y45F10D.2 /REP_DB=WormBase Gene ID /WP=CE16641 /TR=O45945 /GB=CAA16377.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070253(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070253 NCBI Caenorhabditis elegans Y45F10D.2 (Y45F10D.2) mRNA, complete cds. 11/11 None
SNAP00000025703 ENSEMBL cdna:SNAP chromosome:CEL140:IV:13773683:13774615:-1 11/11 None
GENEFINDER00000025725 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:13773683:13774615:-1 11/11 None
Y45F10D.2 ENSEMBL cdna:known chromosome:CEL140:IV:13773683:13774615:-1 gene:Y45F10D.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:13773688-13774621(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y45F10D.2
Entrez Gene 189924 Entrez gene
SwissProt O45945 EMBL-EBI
WormBase CE16641 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA16377 Hypothetical protein Y45F10D.2 [Caenorhabditis elegans] ref|NP_502654.1| Y45F10D.2 [Caenorhabditis elegans] 3.0E-66
blast CAE74589 Hypothetical protein CBG22370 [Caenorhabditis briggsae] 4.0E-40
Sequence
Target Sequence 
>C. ELEGANS:184443_AT
gattcatcgtgttcgcattgttcgctaatattcaatcggcaccggtagttggtctcaact
acgacaatattcaatatcttcataatcttagtctcacttattctaacaactctttggaag
gtctcggagcaattgataatttcaccgacgtgaactcacaaatggctcaaagcctgaagt
ctgcaatcaactcggctctgccaggatatcttgtgtcatcccgttcttcaactccgcctg
cctcctcggcttcttcctcggcttcatccatgacgccaactggaaatggagcacaagctt
ccactattccaatgaatcaaggatcatctactagtatgccagtccgtcaaaaaagacaag
ctgtttcaacaacaacaacaactgcatctccaatgagcacatcctcaccaactccatccc
aacaaccatcaaccactcaaccctacggaactgatgaggaattctatgctcgtaccgatc
tcgtccacttccacgtggatat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTCATCGTGTTCGCATTGTTCGC 540 431 20 Antisense
AATCGGCACCGGTAGTTGGTCTCAA 313 169 53 Antisense
GTCTCACTTATTCTAACAACTCTTT 139 469 110 Antisense
TGAAGTCTGCAATCAACTCGGCTCT 584 575 194 Antisense
GGCTCTGCCAGGATATCTTGTGTCA 173 539 213 Antisense
TGGAGCACAAGCTTCCACTATTCCA 442 549 306 Antisense
CATCTACTAGTATGCCAGTCCGTCA 381 213 344 Antisense
CTGCATCTCCAATGAGCACATCCTC 18 239 401 Antisense
CCACTCAACCCTACGGAACTGATGA 428 275 452 Antisense
GGAATTCTATGCTCGTACCGATCTC 578 531 477 Antisense
ATCTCGTCCACTTCCACGTGGATAT 267 33 497 Antisense
Sequence Source Affymetrix Proprietary Database