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[184433_at]

Description K04F10.1 /REP_DB=WormBase Gene ID /WP=CE11722 /TR=O44771 /GB=AAB96750.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 16      6grp_high1_10_10 > 61      4grp_highest_8_8 > 39      4grp_high_8_8 > 04      6grp_highest_nonorm > 80      6grp_total > 60      4grp_total > 04      6grp_highest_sub > 03_04      6grp_high1_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 152
1 2 3 4 range
A 216.5
(-0.9)
P 		221.6
(-0.6)
P 		180.1
(-3)
P 		285.7
(3)
P 		106
B 222.2
(-1.1)
P 		173.8
(-3)
P 		206.2
(-1.7)
P 		325.3
(3)
P 		152
C 232.6
(-0.1)
P 		188
(-3)
P 		190.8
(-2.8)
P 		281.6
(3)
P 		94
avr 223.8
(-1.2) 		194.5
(-2.9) 		192.4
(-3) 		297.5
(3) 		105

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 154
1 2 3 4 5 6 range
A 176.4
(-0.7)
P 		186.5
(-0.3)
P 		207.2
(0.6)
P 		263.6
(3)
P 		123.9
(-3)
P 		161.4
(-1.4)
P 		140
B 183.5
(-1.1)
P 		146.9
(-3)
P 		265.5
(3)
P 		228
(1.1)
P 		183.2
(-1.2)
P 		160.9
(-2.3)
P 		119
C 195.9
(0)
P 		196
(0)
P 		272.3
(3)
P 		262.8
(2.6)
P 		194.6
(-0)
P 		118.1
(-3)
P 		154
avr 185.3
(-0.8) 		176.5
(-1.3) 		248.3
(2.8) 		251.5
(3) 		167.2
(-1.8) 		146.8
(-3) 		105
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 37
1 2 3 4 range
A 78.8
(3)
P 		48.1
(-2)
P 		47.1
(-2.1)
P 		41.8
(-3)
P 		37
B 70.8
(3)
P 		42.9
(-3)
P 		47.8
(-1.9)
P 		69.3
(2.7)
P 		28
C 77
(3)
P 		54
(-1.7)
P 		66.5
(0.9)
P 		47.5
(-3)
P 		30
avr 75.5
(3) 		48.3
(-3) 		53.8
(-1.8) 		52.9
(-2) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 42
1 2 3 4 5 6 range
A 32.5
(-3)
P 		66.3
(2.9)
P 		45.1
(-0.8)
P 		66.9
(3)
P 		37.4
(-2.1)
P 		39.3
(-1.8)
P 		34
B 63.9
(0.5)
P 		48.9
(-3)
P 		64.5
(0.7)
P 		60.4
(-0.3)
P 		74.4
(3)
P 		60
(-0.4)
P 		26
C 72.6
(3)
P 		59.8
(0.7)
P 		69.3
(2.4)
P 		52.8
(-0.6)
P 		63
(1.3)
P 		39.5
(-3)
P 		33
avr 56.3
(1.4) 		58.3
(2.3) 		59.6
(2.8) 		60
(3) 		58.3
(2.3) 		46.3
(-3) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184433_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2298
Sequence Type Exemplar sequence
Representative Public ID K04F10.1 NCBI
Target Description K04F10.1 /REP_DB=WormBase Gene ID /WP=CE11722 /TR=O44771 /GB=AAB96750.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059436(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059436 NCBI Caenorhabditis elegans K04F10.1 (K04F10.1) mRNA, complete cds. 11/11 None
GENEFINDER00000027832 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6373204:6374312:1 11/11 None
K04F10.1 ENSEMBL cdna:known chromosome:CEL140:I:6373259:6374312:1 gene:K04F10.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6373520-6374241(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K04F10.1
Entrez Gene 187002 Entrez gene
SwissProt O44771 EMBL-EBI
WormBase CE11722 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1632190_AT dm
DROSGENOME1:144619_AT dm
Protein Similarities
Method ID Description E-Value
blast AAB96750 Hypothetical protein K04F10.1 [Caenorhabditis elegans] ref|NP_491837.1| K04F10.1 [Caenorhabditis elegans] 1.0E-106
blast AAB96750 Hypothetical protein K04F10.1 [Caenorhabditis elegans] ref|NP_491837.1| K04F10.1 [Caenorhabditis elegans] 1.0E-105
blast CAE67172 Hypothetical protein CBG12604 [Caenorhabditis briggsae] 1.0E-62
blast CAE67172 Hypothetical protein CBG12604 [Caenorhabditis briggsae] 2.0E-49
blast XP_425139 PREDICTED: similar to Ataxin-1 (Spinocerebellar ataxia type 1 protein) [Gallus gallus] 2.0E-20
blast CAA58533 ataxin-1 [Mus musculus] sp|P54254|ATX1_MOUSE Ataxin-1 (Spinocerebellar ataxia type 1 protein homolog) 5.0E-20
Sequence
Target Sequence 
>C. ELEGANS:184433_AT
agcttccacttattatcctacacatttcatgcgtggtactcaactcaacgtggcaaatgg
aaatatcaaaaaagtggaagatttgtcatctgatgactttctcagatgtgctgcagaatc
agatgatgttattgtcaatgcttctgtgatcaaatctattaaatcaacagctggaagtgt
tactattatttttgaaactggaattgagaaacaactaataccattgaagtgtcaagtcga
gcacccattctttgtattagggaaaggttggtgcagctgtaatcctcggaaaagtggcga
gaactatggtttggattgtgaaattctccaggtagatgatgtgtgtattgtgttgacacg
aaatgaagacaaaataactgactgtgctgttgatatcgtaactgcagaacgagatttaga
gttattcagccagcgggcggcactcaaagaacaacttgttgatgtttattatgagcgagt
ttctggccgagttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCTTCCACTTATTATCCTACACAT 253 79 27 Antisense
CCTACACATTTCATGCGTGGTACTC 569 265 43 Antisense
GTGGTACTCAACTCAACGTGGCAAA 566 487 59 Antisense
GATGACTTTCTCAGATGTGCTGCAG 546 411 118 Antisense
GAAGTGTCAAGTCGAGCACCCATTC 469 335 252 Antisense
TCGAGCACCCATTCTTTGTATTAGG 164 603 263 Antisense
TTGGTGCAGCTGTAATCCTCGGAAA 666 707 294 Antisense
ACTGACTGTGCTGTTGATATCGTAA 145 99 403 Antisense
TTAGAGTTATTCAGCCAGCGGGCGG 221 679 442 Antisense
CGGGCGGCACTCAAAGAACAACTTG 421 243 460 Antisense
TATGAGCGAGTTTCTGGCCGAGTTT 18 657 496 Antisense
Sequence Source Affymetrix Proprietary Database