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[184430_at]

Description F54D12.6 /REP_DB=WormBase Gene ID /WP=CE17871 /TR=O44830 /GB=AAB94991.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 49      4grp_total > 16      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 72
1 2 3 4 range
A 5.2
(-2.6)
A 		15.1
(2.9)
A 		15.3
(3)
A 		4.5
(-3)
A 		11
B 9
(-3)
A 		14.7
(-1.5)
A 		26.9
(1.6)
A 		32.3
(3)
A 		23
C 8.6
(-3)
A 		21.1
(-1.9)
A 		20.3
(-2)
A 		76
(3)
A 		67
avr 7.6
(-3) 		17
(-1.1) 		20.8
(-0.4) 		37.6
(3) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 31
1 2 3 4 5 6 range
A 25
(2.6)
A 		6.6
(-2.9)
A 		8.2
(-2.4)
A 		6.1
(-3)
A 		26.5
(3)
A 		11.2
(-1.5)
A 		20
B 16.4
(-0.2)
A 		2.8
(-2.8)
A 		2.8
(-2.8)
A 		7.6
(-1.9)
A 		1.9
(-3)
A 		32.8
(3)
A 		31
C 30.2
(3)
A 		2.8
(-3)
A 		5
(-2.5)
A 		4.2
(-2.7)
A 		3.2
(-2.9)
A 		14.6
(-0.4)
A 		27
avr 23.9
(3) 		4.1
(-3) 		5.3
(-2.6) 		6
(-2.4) 		10.5
(-1.1) 		19.5
(1.7) 		20
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 1.9
(-0.8)
A 		3.3
(1.7)
A 		4
(3)
A 		0.7
(-3)
A 		3
B 2.9
(-3)
A 		3.6
(-2)
A 		6.2
(2)
A 		6.9
(3)
A 		4
C 2.8
(-3)
A 		6.1
(-1)
A 		7.1
(-0.4)
A 		12.8
(3)
A 		10
avr 2.5
(-3) 		4.3
(-0.5) 		5.8
(1.6) 		6.8
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 12
1 2 3 4 5 6 range
A 4.6
(-0.1)
A 		2.3
(-2.3)
A 		1.8
(-2.7)
A 		1.5
(-3)
A 		8
(3)
A 		2.7
(-1.9)
A 		7
B 5.7
(-0.4)
A 		0.9
(-2.9)
A 		0.7
(-3)
A 		2
(-2.3)
A 		0.8
(-2.9)
A 		12.2
(3)
A 		12
C 11.2
(3)
A 		0.8
(-3)
A 		1.3
(-2.7)
A 		0.8
(-3)
A 		1
(-2.9)
A 		4.9
(-0.6)
A 		10
avr 7.2
(3) 		1.3
(-3) 		1.3
(-3) 		1.4
(-2.9) 		3.3
(-1) 		6.6
(2.4) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184430_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5081
Sequence Type Exemplar sequence
Representative Public ID F54D12.6 NCBI
Target Description F54D12.6 /REP_DB=WormBase Gene ID /WP=CE17871 /TR=O44830 /GB=AAB94991.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061557(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061557 NCBI Caenorhabditis elegans F54D12.6 (F54D12.6) mRNA, complete cds. 11/11 None
GENEFINDER00000002563 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:1369052:1374991:-1 10/11 None
F54D12.6 ENSEMBL cdna:known chromosome:CEL140:II:1373695:1374991:-1 gene:F54D12.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:1373693-1374990(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F54D12.6
Entrez Gene 186242 Entrez gene
SwissProt O44830 EMBL-EBI
WormBase CE17871 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5158 insulin receptor binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB94995 Hypothetical protein F54D12.5 [Caenorhabditis elegans] ref|NP_493957.2| F54D12.5 [Caenorhabditis elegans] 1.0E-127
blast AAB94991 Hypothetical protein F54D12.6 [Caenorhabditis elegans] ref|NP_493958.1| F54D12.6 [Caenorhabditis elegans] 2.0E-91
blast CAE62961 Hypothetical protein CBG07176 [Caenorhabditis briggsae] 5.0E-74
blast AAB94991 Hypothetical protein F54D12.6 [Caenorhabditis elegans] ref|NP_493958.1| F54D12.6 [Caenorhabditis elegans] 1.0E-73
blast CAE62962 Hypothetical protein CBG07177 [Caenorhabditis briggsae] 4.0E-60
blast CAG06920 unnamed protein product [Tetraodon nigroviridis] 3.0E-13
Protein Domains
Database ID Description E-Value
Pfam IPR002404 EMBL-EBI Insulin receptor substrate-1, PTB 2.3E-50
Sequence
Target Sequence 
>C. ELEGANS:184430_AT
gattctgggcaatcgacgtggcgacgacgagagtctctacactggcgctggacctgccgg
atcgggagcttcccactcgtttcgtgtctatattcatggaaatcgcaagaaaaatgagct
cgttcatgcctggcttcgcgtgacccgaaccacaatcacgctggagatctcgaaaaagga
atgcctagtatggccgctgccactgatccgccgatacggatacacttctgccggaatttt
cttcttcgaatctggaagacggtgcgagtctggagagggaatgttcacgtttcagagcaa
aaaagctgatgagatatttcagctcatccaatcactgatagaagagttcgccaacacgtc
aatggagaaacagaatatggaaaatagtttccggtaccagcgacagcaatccgtaccgat
cagctctcggagaatgtcaaatacgagtaatacgtcatcggttttcggtacggca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTCTGGGCAATCGACGTGGCGAC 694 431 42 Antisense
ACGACGAGAGTCTCTACACTGGCGC 522 91 65 Antisense
TTCCCACTCGTTTCGTGTCTATATT 449 695 111 Antisense
AAAAATGAGCTCGTTCATGCCTGGC 588 129 151 Antisense
AGGAATGCCTAGTATGGCCGCTGCC 291 55 218 Antisense
GCCGATACGGATACACTTCTGCCGG 631 283 251 Antisense
CTTCTGCCGGAATTTTCTTCTTCGA 648 219 266 Antisense
AGATATTTCAGCTCATCCAATCACT 115 67 353 Antisense
AGAAGAGTTCGCCAACACGTCAATG 590 73 381 Antisense
AAATAGTTTCCGGTACCAGCGACAG 27 103 423 Antisense
TACGTCATCGGTTTTCGGTACGGCA 232 647 492 Antisense
Sequence Source Affymetrix Proprietary Database