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[184424_at]

Description F54D12.8 /REP_DB=WormBase Gene ID /WP=CE17873 /TR=O44833 /GB=AAB94989.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 43      4grp_total > 0      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 46
1 2 3 4 range
A 33.3
(0.3)
A 		19
(-1.8)
A 		10.4
(-3)
A 		52.5
(3)
A 		42
B 37.7
(0)
A 		19.3
(-3)
A 		56.1
(3)
A 		39.1
(0.2)
A 		37
C 11.8
(-3)
A 		21.7
(-0.4)
A 		30.3
(1.9)
A 		34.3
(3)
A 		23
avr 27.6
(-0.9) 		20
(-3) 		32.3
(0.4) 		42
(3) 		22

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 49.3
(3)
A 		9.4
(-3)
A 		11
(-2.8)
A 		14.9
(-2.2)
A 		26.4
(-0.4)
A 		30
(0.1)
A 		40
B 11.8
(-2.7)
A 		9.8
(-3)
A 		48.4
(3)
A 		33.3
(0.7)
A 		35.3
(1)
A 		26.2
(-0.5)
A 		39
C 43.2
(1.5)
A 		32.3
(0.2)
A 		54.9
(3)
A 		16.8
(-1.7)
A 		38.4
(0.9)
A 		6.7
(-3)
A 		48
avr 34.8
(2.1) 		17.2
(-3) 		38.1
(3) 		21.7
(-1.7) 		33.4
(1.7) 		21
(-1.9) 		21
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 12.1
(3)
A 		4.1
(-2.1)
A 		2.7
(-3)
A 		7.7
(0.2)
A 		9
B 12
(2.3)
A 		4.8
(-3)
A 		13
(3)
A 		8.3
(-0.4)
A 		8
C 3.9
(-3)
A 		6.2
(-0.9)
A 		10.6
(3)
A 		5.8
(-1.3)
A 		7
avr 9.3
(3) 		5
(-3) 		8.8
(2.3) 		7.3
(0.2) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 9.1
(3)
A 		3.3
(-2.2)
A 		2.4
(-3)
A 		3.8
(-1.7)
A 		8
(2)
A 		7.3
(1.4)
A 		7
B 4.1
(-2.5)
A 		3.2
(-3)
A 		11.8
(1.6)
A 		8.8
(0)
A 		14.4
(3)
A 		9.8
(0.5)
A 		11
C 16
(3)
A 		9.8
(0.3)
A 		14
(2.1)
A 		3.4
(-2.5)
A 		12.4
(1.4)
A 		2.3
(-3)
A 		14
avr 9.7
(1.2) 		5.4
(-2.9) 		9.4
(0.9) 		5.3
(-3) 		11.6
(3) 		6.5
(-1.9) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184424_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4085
Sequence Type Exemplar sequence
Representative Public ID F54D12.8 NCBI
Target Description F54D12.8 /REP_DB=WormBase Gene ID /WP=CE17873 /TR=O44833 /GB=AAB94989.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061560(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061560 NCBI Caenorhabditis elegans F54D12.8 (F54D12.8) mRNA, complete cds. 11/11 None
SNAP00000002559 ENSEMBL cdna:SNAP chromosome:CEL140:II:1394970:1396607:-1 11/11 None
GENEFINDER00000002565 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:1394970:1398044:-1 11/11 None
F54D12.8 ENSEMBL cdna:known chromosome:CEL140:II:1395010:1398044:-1 gene:F54D12.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:1395008-1398043(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F54D12.8
Entrez Gene 186243 Entrez gene
SwissProt O44833 EMBL-EBI
WormBase CE17873 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB94989 Hypothetical protein F54D12.8 [Caenorhabditis elegans] ref|NP_493961.1| F54D12.8 [Caenorhabditis elegans] 0.0
blast AAB94989 Hypothetical protein F54D12.8 [Caenorhabditis elegans] ref|NP_493961.1| F54D12.8 [Caenorhabditis elegans] 1.0E-149
blast AAB94989 Hypothetical protein F54D12.8 [Caenorhabditis elegans] ref|NP_493961.1| F54D12.8 [Caenorhabditis elegans] 1.0E-116
blast AAR30205 Hypothetical protein F54D12.7 [Caenorhabditis elegans] ref|NP_493959.2| F54D12.7 [Caenorhabditis elegans] 6.0E-39
blast CAE62970 Hypothetical protein CBG07187 [Caenorhabditis briggsae] 1.0E-36
blast CAE62970 Hypothetical protein CBG07187 [Caenorhabditis briggsae] 3.0E-22
blast AAY55830 Hypothetical protein F54D12.11 [Caenorhabditis elegans] ref|NP_001022195.1| F54D12.11 [Caenorhabditis elegans] 1.0E-19
blast AAB94992 Hypothetical protein F54D12.1 [Caenorhabditis elegans] ref|NP_493963.3| F54D12.1 [Caenorhabditis elegans] 2.0E-14
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000002559 1 283-305
GENEFINDER00000002565 1 391-413
Sequence
Target Sequence 
>C. ELEGANS:184424_AT
atgtgcctgttcgagtgtagctctcggcctgattgttttatggccgctgtggacttgggg
agtaacaagtgcaaagtttacaactatggaggtgtcgatcaatttgagtactctgatgca
ccaacaacgtttgcaggtggtttaaagtatactccggccagctacacatgttcttcaaac
ttcagctctctttccgatggacgttatgttcacactctgacatccacaccatactcatca
tatcaatacaatggtactgcgttcattcggatgccctggcctatcaaacatcctcaatcg
tactcaactgcaatctgtgtagcatttggggcagatggactgatgccgattcccggcgcg
ggtggttatgatgttttctatgcgattaaacccctacaaagaacagtggatgttggatac
gtgcgagataatacaactaaacaatatttctggctgcaaccacagcttaggcttgacgac
tcgcccgtaatttggaatcatgataacccgtttaatgaacatgattgctcacttcttgtt
attggcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGTGCCTGTTCGAGTGTAGCTCTC 593 49 394 Antisense
AGCTCTCGGCCTGATTGTTTTATGG 570 79 412 Antisense
TTTATGGCCGCTGTGGACTTGGGGA 358 671 430 Antisense
TCAGCTCTCTTTCCGATGGACGTTA 336 625 575 Antisense
TATGTTCACACTCTGACATCCACAC 112 657 598 Antisense
AAACATCCTCAATCGTACTCAACTG 294 127 679 Antisense
GCAGATGGACTGATGCCGATTCCCG 413 311 724 Antisense
TCCCGGCGCGGGTGGTTATGATGTT 442 595 744 Antisense
GCAACCACAGCTTAGGCTTGACGAC 263 321 849 Antisense
GACGACTCGCCCGTAATTTGGAATC 233 375 868 Antisense
GATTGCTCACTTCTTGTTATTGGCG 673 433 916 Antisense
Sequence Source Affymetrix Proprietary Database