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[184378_at]

Description F54D12.5 /REP_DB=WormBase Gene ID /WP=CE17870 /TR=O44829 /GB=AAB94995.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 08      4grp_total > 60      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 102
1 2 3 4 range
A 100.2
(-2.3)
P 		96
(-3)
A 		130.6
(3)
A 		105.7
(-1.3)
A 		35
B 81
(3)
M 		73.3
(2.1)
A 		76.5
(2.5)
A 		28.4
(-3)
A 		53
C 77.6
(-0.9)
A 		93.9
(3)
A 		81.7
(0.1)
A 		68.7
(-3)
P 		25
avr 86.3
(0.9) 		87.7
(1.2) 		96.3
(3) 		67.6
(-3) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 74
1 2 3 4 5 6 range
A 76.5
(0.8)
A 		93.5
(3)
A 		51.8
(-2.3)
A 		46.2
(-3)
A 		66.8
(-0.4)
A 		46.5
(-3)
A 		47
B 51
(0.8)
A 		20.5
(-3)
A 		47.9
(0.4)
A 		69.2
(3)
A 		32
(-1.6)
A 		59.9
(1.9)
P 		49
C 88.6
(2)
A 		76.6
(0.1)
A 		68.7
(-1.2)
A 		94.8
(3)
A 		57.1
(-3)
A 		61.5
(-2.3)
P 		38
avr 72
(3) 		63.5
(0.5) 		56.1
(-1.8) 		70.1
(2.4) 		52
(-3) 		56
(-1.8) 		20
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 30
1 2 3 4 range
A 36.5
(3)
P 		20.8
(-1.5)
A 		34.2
(2.3)
A 		15.5
(-3)
A 		21
B 25.8
(3)
M 		18.1
(0.7)
A 		17.7
(0.5)
A 		6.1
(-3)
A 		20
C 25.7
(2)
A 		27
(2.5)
A 		28.5
(3)
A 		11.6
(-3)
P 		17
avr 29.3
(3) 		22
(0.6) 		26.8
(2.2) 		11.1
(-3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 27
1 2 3 4 5 6 range
A 14.1
(-2.2)
A 		33.3
(3)
A 		11.3
(-3)
A 		11.7
(-2.9)
A 		20.1
(-0.6)
A 		11.3
(-3)
A 		22
B 17.7
(1.2)
A 		6.8
(-3)
A 		11.6
(-1.1)
A 		18.3
(1.5)
A 		13
(-0.6)
A 		22.3
(3)
P 		16
C 32.8
(3)
A 		23.4
(-0.7)
A 		17.5
(-3)
A 		19.1
(-2.4)
A 		18.5
(-2.6)
A 		20.6
(-1.8)
P 		15
avr 21.5
(3) 		21.2
(2.8) 		13.5
(-3) 		16.4
(-0.8) 		17.2
(-0.2) 		18.1
(0.5) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184378_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4897
Sequence Type Exemplar sequence
Representative Public ID F54D12.5 NCBI
Target Description F54D12.5 /REP_DB=WormBase Gene ID /WP=CE17870 /TR=O44829 /GB=AAB94995.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061556(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061556 NCBI Caenorhabditis elegans F54D12.5 (F54D12.5) mRNA, complete cds. 11/11 None
GENEFINDER00000002563 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:1369052:1374991:-1 11/11 None
F54D12.5 ENSEMBL cdna:known chromosome:CEL140:II:1369052:1372618:-1 gene:F54D12.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:1369792-1372742(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F54D12.5
Entrez Gene 3565154 Entrez gene
SwissProt O44829 EMBL-EBI
WormBase CE36864 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB94995 Hypothetical protein F54D12.5 [Caenorhabditis elegans] ref|NP_493957.2| F54D12.5 [Caenorhabditis elegans] 1.0E-127
blast CAE62961 Hypothetical protein CBG07176 [Caenorhabditis briggsae] 2.0E-74
blast CAE62961 Hypothetical protein CBG07176 [Caenorhabditis briggsae] 5.0E-74
blast AAB94991 Hypothetical protein F54D12.6 [Caenorhabditis elegans] ref|NP_493958.1| F54D12.6 [Caenorhabditis elegans] 1.0E-73
Protein Domains
Database ID Description E-Value
Pfam IPR002404 EMBL-EBI Insulin receptor substrate-1, PTB 2.3E-50
Sequence
Target Sequence 
>C. ELEGANS:184378_AT
ctgaagtgtcggctgcactttcagcgatgaatcgtgacatttcctctggctccggctctt
ccacggctgcttcagccaccgccaccacgtcatcaaaactgccacgttgcacgccaccgc
ccgtctcgaaccggcagctaaaacggtatgcaatggtaccgggaacatccatgtcgaagc
gtcgaatctcacgggaagacgcgaaacgggcgttcctgtcgagtgatccacgtgtcggac
acgtcattgatgatccgagatatgcggctgtgaatgtggaggctgatgagaattatgcga
atttacgcgatattcagactgacaaaatcatgcgtggaatcgacgtggaaaccctcacca
acctacacacaaccactgaaccatctcccccactgccacgtcatcaccagattcaggcct
ccaccaccgccggtgccacgtcatcatcattatctgctgctgctggaacaagaggaacac
taaaaccgtcgaatggcctaatgaccgcccgtgcggcgacggcttctcccctgccacgtc
tcaactatgcacaaatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTGAAGTGTCGGCTGCACTTTCAGC 686 235 374 Antisense
ATGAATCGTGACATTTCCTCTGGCT 47 45 400 Antisense
CATCAAAACTGCCACGTTGCACGCC 362 211 464 Antisense
GTACCGGGAACATCCATGTCGAAGC 516 457 529 Antisense
GTCGAAGCGTCGAATCTCACGGGAA 325 473 546 Antisense
TCGAGTGATCCACGTGTCGGACACG 40 597 592 Antisense
TGGAATCGACGTGGAAACCCTCACC 564 545 708 Antisense
GCCACGTCATCACCAGATTCAGGCC 675 279 768 Antisense
GTCATCATCATTATCTGCTGCTGCT 445 467 813 Antisense
CCGTCGAATGGCCTAATGACCGCCC 638 263 859 Antisense
GCCACGTCTCAACTATGCACAAATT 677 279 906 Antisense
Sequence Source Affymetrix Proprietary Database