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[184369_s_at]

Description T22D1.10 /REP_DB=WormBase Gene ID /WP=CE17254 /TR=Q9GZH2 /GB=AAF98631.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 68      6grp_high1_10_10 > 96      4grp_highest_8_8 > 33      4grp_high_8_8 > 58      6grp_highest_nonorm > 95      6grp_total > 86      4grp_total > 61      6grp_highest_sub > In      6grp_cgc > 68      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 550
1 2 3 4 range
A 994.5
(3)
P 		905.9
(1.9)
P 		913.7
(2)
P 		525.6
(-3)
P 		469
B 978
(2.3)
P 		904.3
(1.1)
P 		1019
(3)
P 		657.1
(-3)
P 		362
C 1075.2
(3)
P 		866.3
(-0.1)
P 		904.9
(0.5)
P 		671
(-3)
P 		404
avr 1015.9
(3) 		892.2
(1.1) 		945.9
(1.9) 		617.9
(-3) 		398

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 732
1 2 3 4 5 6 range
A 485.5
(-3)
P 		537
(-2.5)
P 		681.5
(-0.9)
P 		688.4
(-0.9)
P 		883.5
(1.2)
P 		1051.8
(3)
P 		566
B 512.5
(-3)
P 		589.3
(-2.3)
P 		567.5
(-2.5)
P 		722.5
(-1.2)
P 		873.9
(0.1)
P 		1217
(3)
P 		705
C 531.9
(-3)
P 		580.8
(-2.5)
P 		726.3
(-0.9)
P 		880
(0.7)
P 		731.4
(-0.9)
P 		1098.7
(3)
P 		567
avr 510
(-3) 		569
(-2.4) 		658.4
(-1.5) 		763.6
(-0.5) 		829.6
(0.1) 		1122.5
(3) 		613
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 285
1 2 3 4 range
A 362.1
(3)
P 		196.7
(-0.5)
P 		238.9
(0.4)
P 		76.9
(-3)
P 		285
B 311.9
(3)
P 		223.3
(-0.1)
P 		236.1
(0.4)
P 		140
(-3)
P 		172
C 355.9
(3)
P 		249.1
(0.4)
P 		315.4
(2)
P 		113.1
(-3)
P 		243
avr 343.3
(3) 		223
(-0.1) 		263.5
(0.9) 		110
(-3) 		233

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 364
1 2 3 4 5 6 range
A 89.5
(-3)
P 		191
(0.4)
P 		148.4
(-1)
P 		174.6
(-0.1)
P 		266.6
(3)
P 		256.1
(2.6)
P 		177
B 178.3
(-2.2)
P 		196.2
(-1.9)
P 		137.8
(-3)
P 		191.4
(-2)
P 		355.1
(1.1)
P 		453.8
(3)
P 		316
C 197.2
(-2.4)
P 		177.2
(-3)
P 		184.9
(-2.8)
P 		177
(-3)
P 		236.7
(-1.1)
P 		367.8
(3)
P 		191
avr 155
(-3) 		188.1
(-2) 		157
(-2.9) 		181
(-2.2) 		286.1
(0.9) 		359.2
(3) 		204
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184369_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8695
Sequence Type Exemplar sequence
Representative Public ID T22D1.10 NCBI
Target Description T22D1.10 /REP_DB=WormBase Gene ID /WP=CE17254 /TR=Q9GZH2 /GB=AAF98631.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068666(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068666 NCBI Caenorhabditis elegans RUVB (recombination protein) homolog family member (ruvb-2) (ruvb-2) mRNA, complete cds. 11/11 None
SNAP00000024012 ENSEMBL cdna:SNAP chromosome:CEL140:IV:6917265:6919382:-1 11/11 None
GENEFINDER00000024027 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:6917265:6919382:-1 11/11 None
T22D1.10 ENSEMBL cdna:known chromosome:CEL140:IV:6917124:6919390:-1 gene:T22D1.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:6917270-6919388(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ruvb-2
Ensembl T22D1.10
Entrez Gene 177458 Entrez gene
SwissProt Q9GZH2 EMBL-EBI
WormBase CE17254 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:247014_AT DNA helicase, putative at
CANINE:1591959_AT similar to RuvB-like 2 (48-kDa TATA box-binding protein-interacting protein) (48-kDa TBP-interacting protein) (TIP49b) (Repressing pontin 52) (Reptin 52) (51 kDa erythrocyte cytosolic protein) (ECP-51) (TIP60-associated protein 54-beta) (TAP54-beta... cfa
CANINE_2:CFA.19239.1.S1_S_AT similar to RuvB-like 2 (48-kDa TATA box-binding protein-interacting protein) (48-kDa TBP-interacting protein) (TIP49b) (Repressing pontin 52) (Reptin 52) (51 kDa erythrocyte cytosolic protein) (ECP-51) (TIP60-associated protein 54-beta) (TAP54-beta... cfa
CANINE_2:CFA.20388.1.S1_S_AT similar to RuvB-like 2 (48-kDa TATA box-binding protein-interacting protein) (48-kDa TBP-interacting protein) (TIP49b) (Repressing pontin 52) (Reptin 52) (51 kDa erythrocyte cytosolic protein) (ECP-51) (TIP60-associated protein 54-beta) (TAP54-beta... cfa
CANINE_2:CFAAFFX.6720.1.S1_AT similar to RuvB-like 2 (48-kDa TATA box-binding protein-interacting protein) (48-kDa TBP-interacting protein) (TIP49b) (Repressing pontin 52) (Reptin 52) (51 kDa erythrocyte cytosolic protein) (ECP-51) (TIP60-associated protein 54-beta) (TAP54-beta... cfa
DROSOPHILA_2:1630792_AT reptin dm
DROSGENOME1:153759_AT reptin dm
HG-FOCUS:201459_AT RuvB-like 2 (E. coli) hs
HG-U133_PLUS_2:201459_AT RuvB-like 2 (E. coli) hs
HG-U133A:201459_AT RuvB-like 2 (E. coli) hs
HG-U133A_2:201459_AT RuvB-like 2 (E. coli) hs
HU35KSUBA:RC_AA457129_AT RuvB-like 2 (E. coli) hs
HU35KSUBA:W74106_AT RuvB-like 2 (E. coli) hs
U133_X3P:G5730022_3P_S_AT RuvB-like 2 (E. coli) hs
U133_X3P:G5730022_3P_AT RuvB-like 2 (E. coli) hs
U133_X3P:HS2.403943.1.A1_3P_X_AT RuvB-like 2 (E. coli) hs
U133_X3P:HS2.403943.1.A1_3P_AT RuvB-like 2 (E. coli) hs
HG-U133_PLUS_2:1559945_AT RuvB-like 2 (E. coli) hs
HG-U133_PLUS_2:1559946_S_AT RuvB-like 2 (E. coli) hs
HG-U95AV2:35758_AT RuvB-like 2 (E. coli) hs
MG-U74AV2:160422_AT RuvB-like protein 2 mm
MG-U74CV2:167710_I_AT RuvB-like protein 2 mm
MOE430A:1422482_AT RuvB-like protein 2 mm
MOUSE430A_2:1422482_AT RuvB-like protein 2 mm
MOUSE430_2:1422482_AT RuvB-like protein 2 mm
MU19KSUBC:TC36730_AT RuvB-like protein 2 (Ruvbl2), mRNA mm
MU19KSUBC:TC36730_G_AT RuvB-like protein 2 (Ruvbl2), mRNA mm
MU19KSUBC:TC41024_AT RuvB-like protein 2 (Ruvbl2), mRNA mm
YEAST_2:1769758_AT Essential protein involved in transcription regulation; component of chromatin remodeling complexes; required for assembly and function of the INO80 complex; member of the RUVB-like protein family Sc
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
166 nucleotide binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF98631 Ruvb (recombination protein) homolog protein 2 [Caenorhabditis elegans] ref|NP_501067.1| RUVB (recombination protein) homolog family member (ruvb-2) [Caenorhabditis elegans] 0.0
blast CAE61760 Hypothetical protein CBG05719 [Caenorhabditis briggsae] 0.0
blast AAH47966 Ruvbl2-prov protein [Xenopus laevis] 1.0E-155
Protein Domains
Database ID Description E-Value
Pfam IPR010339 EMBL-EBI TIP49, C-terminal 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:184369_S_AT
tgacgtcatcaattctcgtacccaaggatacgtggcactcttctcaggagacactggaga
gatcaaagcagaagtgagagatcagattaataagaaggttttggagtggagagaagaggg
aaaagcaaagtttgtaccaggagtattgtttattgatgaagctcatatgcttgatatcga
atgcttttcgttcctcaacagagccatcgaaggcgaactcagtcccttgattatcatggc
caccaacagattgatcgaaaaagtgcgtggaacagatgtcgaatctgctcatggcatccc
atctgatttccttgatcgtatgctcatcatcaacgcgatcccatatactaaagaagatac
agcaaagattttgtcaattcgatgtgatgaagaaggagtcaaattgcaaccaactgccct
tgatcttctagtgaaattacaagaagccacatctcttcgttactgtattcatctcattgc
tgcttctgaagttattcgtattcgcagtaaagctgaaattgtcaccactgatcacattgg
atcagcttatcgtttgttcttcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGACGTCATCAATTCTCGTACCCAA 149 571 741 Antisense
CAAGGATACGTGGCACTCTTCTCAG 6 183 763 Antisense
TTTCGTTCCTCAACAGAGCCATCGA 455 671 926 Antisense
TCGAAGGCGAACTCAGTCCCTTGAT 447 601 947 Antisense
GTCCCTTGATTATCATGGCCACCAA 474 473 962 Antisense
ATCCCATCTGATTTCCTTGATCGTA 469 37 1036 Antisense
GATCGTATGCTCATCATCAACGCGA 572 417 1054 Antisense
ATCAACGCGATCCCATATACTAAAG 380 27 1069 Antisense
AACTGCCCTTGATCTTCTAGTGAAA 2 143 1152 Antisense
AAGAAGCCACATCTCTTCGTTACTG 184 153 1181 Antisense
GGATCAGCTTATCGTTTGTTCTTCG 203 511 1279 Antisense
Sequence Source Affymetrix Proprietary Database