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[184356_s_at]

Description F15B10.2 /REP_DB=WormBase Gene ID /WP=CE16988 /TR=O44165 /GB=AAB88350.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 56      6grp_high1_10_10 > 75      4grp_highest_8_8 > 21      4grp_high_8_8 > 15      6grp_highest_nonorm > 84      6grp_total > 65      4grp_total > 0      6grp_cgc > 66      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 139
1 2 3 4 range
A 458.8
(-3)
P 		479
(-0.4)
P 		505.1
(3)
P 		488.2
(0.8)
P 		46
B 416
(-1.7)
P 		412.2
(-1.9)
P 		387
(-3)
P 		522.7
(3)
P 		136
C 436.5
(-0.2)
P 		383.6
(-3)
P 		484.9
(2.3)
P 		498.5
(3)
P 		115
avr 437.1
(-2.1) 		424.9
(-3) 		459
(-0.4) 		503.1
(3) 		78

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 411
1 2 3 4 5 6 range
A 469.9
(-3)
P 		676.9
(3)
P 		540.2
(-1)
P 		621.4
(1.4)
P 		574.8
(0)
P 		645.8
(2.1)
P 		207
B 546.8
(-1.8)
P 		551.8
(-1.7)
P 		644.9
(0.2)
P 		761.5
(2.5)
P 		484.7
(-3)
P 		784.1
(3)
P 		299
C 445.1
(-3)
P 		631.3
(-0.3)
P 		774.4
(1.8)
P 		597.3
(-0.8)
P 		501.5
(-2.2)
P 		855.8
(3)
P 		411
avr 487.3
(-3) 		620
(-0.1) 		653.2
(0.6) 		660.1
(0.8) 		520.3
(-2.3) 		761.9
(3) 		275
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 98
1 2 3 4 range
A 167.1
(3)
P 		104
(-1)
P 		132.1
(0.8)
P 		71.5
(-3)
P 		96
B 132.7
(3)
P 		101.8
(-1.3)
P 		89.7
(-3)
P 		111.4
(0)
P 		43
C 144.5
(1.3)
P 		110.3
(-1.1)
P 		169
(3)
P 		84.1
(-3)
P 		85
avr 148.1
(3) 		105.4
(-1.3) 		130.3
(1.2) 		89
(-3) 		59

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 206
1 2 3 4 5 6 range
A 86.6
(-3)
P 		240.7
(3)
P 		117.6
(-1.8)
P 		157.6
(-0.2)
P 		173.5
(0.4)
P 		157.2
(-0.3)
P 		154
B 190.3
(-1.5)
P 		183.7
(-1.8)
P 		156.6
(-3)
P 		201.7
(-1)
P 		196.9
(-1.2)
P 		292.4
(3)
P 		136
C 165
(-1.4)
P 		192.6
(-0.4)
P 		197.1
(-0.2)
P 		120.1
(-3)
P 		162.3
(-1.5)
P 		286.5
(3)
P 		166
avr 147.3
(-3) 		205.7
(0.6) 		157.1
(-2.4) 		159.8
(-2.2) 		177.6
(-1.1) 		245.4
(3) 		98
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184356_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10386
Sequence Type Exemplar sequence
Representative Public ID F15B10.2 NCBI
Target Description F15B10.2 /REP_DB=WormBase Gene ID /WP=CE16988 /TR=O44165 /GB=AAB88350.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068617(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068617 NCBI Caenorhabditis elegans Dicer Related Helicase family member (drh-1) (drh-1) mRNA, complete cds. 11/11 None
SNAP00000005367 ENSEMBL cdna:SNAP chromosome:CEL140:IV:6607541:6608986:-1 11/11 None
GENEFINDER00000005370 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:6607541:6613332:-1 11/11 None
F15B10.2.1 ENSEMBL cdna:known chromosome:CEL140:IV:6606952:6613337:-1 gene:F15B10.2 11/11 None
F15B10.2.2 ENSEMBL cdna:known chromosome:CEL140:IV:6607370:6613337:-1 gene:F15B10.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:6607546-6613338(-) 99.97 100.0
Public Domain and Genome References
Gene Title
Gene Symbol drh-1
Ensembl F15B10.2
Entrez Gene 177425 Entrez gene
SwissProt O44165 EMBL-EBI
WormBase CE16988 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.16351.1.S1_S_AT similar to Interferon induced with helicase C domain protein 1 (Helicase with 2 CARD domains) (Helicard) (Melanoma differentiation-associated protein 5) (MDA-5) (RNA helicase-DEAD box protein 116) (Murabutide-down-regulated protein)... cfa
CHICKEN:GGA.16457.1.S1_S_AT interferon induced with helicase C domain 1 gga
CHICKEN:GGAAFFX.23449.1.S1_AT interferon induced with helicase C domain 1 gga
HU35KSUBB:RC_AA134958_AT interferon induced with helicase C domain 1 hs
HG-U133A:219209_AT interferon induced with helicase C domain 1 hs
HG-U133_PLUS_2:219209_AT interferon induced with helicase C domain 1 hs
HG-FOCUS:219209_AT interferon induced with helicase C domain 1 hs
HG-U133A_2:219209_AT interferon induced with helicase C domain 1 hs
U133_X3P:G11545921_3P_AT interferon induced with helicase C domain 1 hs
U133_X3P:HS2AFFX.1.318.S1_3P_AT interferon induced with helicase C domain 1 hs
HG-U133_PLUS_2:1555464_AT interferon induced with helicase C domain 1 hs
HG-U95B:51920_AT interferon induced with helicase C domain 1 hs
HG-U133_PLUS_2:216020_AT Interferon induced with helicase C domain 1 hs
HG-U133A_2:216020_AT Interferon induced with helicase C domain 1 hs
HG-U133A:216020_AT Interferon induced with helicase C domain 1 hs
HG-U95AV2:33612_AT Interferon induced with helicase C domain 1 hs
U133_X3P:HS.163173.0.A1_3P_AT Interferon induced with helicase C domain 1 hs
MOUSE430A_2:1426276_AT interferon induced with helicase C domain 1 mm
MOE430A:1426276_AT interferon induced with helicase C domain 1 mm
MOUSE430_2:1426276_AT interferon induced with helicase C domain 1 mm
MU11KSUBA:AA177851_S_AT interferon induced with helicase C domain 1 mm
MG-U74AV2:103446_AT interferon induced with helicase C domain 1 mm
MG-U74CV2:133277_AT interferon induced with helicase C domain 1 mm
RG-U34B:RC_AI058349_AT Similar to HELICARD rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
9307 DNA restriction-modification system inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
4386 helicase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
8026 ATP-dependent helicase activity inferred from electronic annotation QuickGO AmiGO
15668 type III site-specific deoxyribonuclease activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB88350 Dicer related helicase protein 1 [Caenorhabditis elegans] ref|NP_501018.1| Dicer Related Helicase family member (drh-1) [Caenorhabditis elegans] gb|AAM76083.1| dicer-related helicase [Caenorhabditis elegans] 0.0
blast CAE61501 Hypothetical protein CBG05400 [Caenorhabditis briggsae] 0.0
blast A88708 protein C01B10.1 [imported] - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 2.0E-11
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 1.6E-19
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 1.6E-19
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000005370 1 974-996
Sequence
Target Sequence 
>C. ELEGANS:184356_S_AT
gtctgagacaatctccctaatccaaaattcgccagctgagttccgaaaatgtgtagacga
ggaaagcaataaaatatggcctcgaatcctgagagaagataccgataaagcgcaaaaaat
cgaggagcaaattaatcgaaatattgtatacaagatcatctgtaaaaaatgtgaagcgat
tttgtgtacaagcaaggatattcgatcaagaaatactcaataccttgtctgtgatccggg
attctggagcctcgttcgaaaaactcgcctgacggatgagcaacaagcattaattaaata
taatgcaactggatcgattaattgtcgccgcgaaaattgtggtttaaagcttggtcagtt
gattgaagtgaatacagtggatcttccatgtctatcagctctttctatcgtgttgctagt
cgaaggtaccgataaacggatcatcgtcaagaaatggaaaaatattcttgacaaatattt
cacaccgaccgaaatacgacaattggatgttcaaacaatgagagatgcggatcaagctcg
aacaccaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCTGAGACAATCTCCCTAATCCAA 650 469 2523 Antisense
TCCAAAATTCGCCAGCTGAGTTCCG 591 587 2543 Antisense
AATATGGCCTCGAATCCTGAGAGAA 293 171 2595 Antisense
TACTCAATACCTTGTCTGTGATCCG 373 645 2736 Antisense
CTGTGATCCGGGATTCTGGAGCCTC 1 241 2751 Antisense
TGGAGCCTCGTTCGAAAAACTCGCC 315 547 2767 Antisense
AAAACTCGCCTGACGGATGAGCAAC 145 131 2782 Antisense
GGATCGATTAATTGTCGCCGCGAAA 154 513 2833 Antisense
AGTGGATCTTCCATGTCTATCAGCT 426 59 2898 Antisense
CACACCGACCGAAATACGACAATTG 516 195 3003 Antisense
ATGCGGATCAAGCTCGAACACCAAT 557 41 3047 Antisense
Sequence Source Affymetrix Proprietary Database