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[184354_at]

Description Y73F8A.35 /REP_DB=WormBase Gene ID /WP=CE24655 /TR=Q9NA43 /GB=CAB60564.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 25      4grp_total > 39      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 29
1 2 3 4 range
A 20.9
(3)
A 		8.5
(-1.8)
A 		5.4
(-3)
A 		14.7
(0.6)
A 		16
B 32.7
(3)
A 		8.7
(-2.6)
A 		7.2
(-3)
A 		13.7
(-1.5)
A 		26
C 34.5
(3)
A 		5.3
(-3)
A 		6.4
(-2.8)
A 		15.2
(-1)
A 		29
avr 29.4
(3) 		7.5
(-2.7) 		6.3
(-3) 		14.5
(-0.9) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 19.3
(3)
A 		12.9
(0.7)
A 		2.7
(-3)
A 		5.8
(-1.9)
A 		6.3
(-1.7)
A 		3
(-2.9)
A 		17
B 4.3
(-2.3)
A 		9.1
(3)
A 		5.7
(-0.8)
A 		4.8
(-1.8)
A 		3.7
(-3)
A 		5.3
(-1.2)
A 		5
C 6.2
(-1.5)
A 		8.4
(-0.2)
A 		13.7
(3)
A 		4.7
(-2.5)
A 		3.8
(-3)
A 		4.8
(-2.4)
A 		10
avr 9.9
(2.8) 		10.1
(3) 		7.4
(0.2) 		5.1
(-2.3) 		4.6
(-2.8) 		4.4
(-3) 		6
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 7.6
(3)
A 		1.8
(-2.6)
A 		1.4
(-3)
A 		2.2
(-2.2)
A 		6
B 10.4
(3)
A 		2.2
(-2.7)
A 		1.7
(-3)
A 		2.9
(-2.2)
A 		9
C 11.4
(3)
A 		1.5
(-3)
A 		2.2
(-2.6)
A 		2.6
(-2.3)
A 		10
avr 9.8
(3) 		1.8
(-3) 		1.8
(-3) 		2.6
(-2.4) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4
1 2 3 4 5 6 range
A 3.5
(1.4)
A 		4.6
(3)
A 		0.6
(-3)
A 		1.5
(-1.7)
A 		1.9
(-1.1)
A 		0.7
(-2.9)
A 		4
B 1.5
(-2.3)
A 		3
(3)
A 		1.4
(-2.6)
A 		1.3
(-3)
A 		1.5
(-2.3)
A 		2
(-0.5)
A 		2
C 2.3
(0.2)
A 		2.6
(0.9)
A 		3.5
(3)
A 		0.9
(-3)
A 		1.2
(-2.3)
A 		1.6
(-1.4)
A 		3
avr 2.4
(0.3) 		3.4
(3) 		1.8
(-1.4) 		1.2
(-3) 		1.5
(-2.2) 		1.4
(-2.5) 		2
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184354_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9152
Sequence Type Exemplar sequence
Representative Public ID Y73F8A.35 NCBI
Target Description Y73F8A.35 /REP_DB=WormBase Gene ID /WP=CE24655 /TR=Q9NA43 /GB=CAB60564.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070469(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070469 NCBI Caenorhabditis elegans Y73F8A.35 (Y73F8A.35) mRNA, complete cds. 11/11 None
SNAP00000030840 ENSEMBL cdna:SNAP chromosome:CEL140:IV:15533308:15536869:-1 10/11 None
GENEFINDER00000030888 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:15532459:15539901:-1 11/11 None
Y73F8A.35 ENSEMBL cdna:known chromosome:CEL140:IV:15532459:15539901:-1 gene:Y73F8A.35 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:15532464-15539907(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y73F8A.35
Entrez Gene 178438 Entrez gene
SwissProt Q9NA43 EMBL-EBI
WormBase CE24655 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB60564 Hypothetical protein Y73F8A.35 [Caenorhabditis elegans] ref|NP_502870.1| Y73F8A.35 [Caenorhabditis elegans] 0.0
blast CAA93678 Hypothetical protein R03G8.3 [Caenorhabditis elegans] ref|NP_510174.2| R03G8.3 [Caenorhabditis elegans] 0.0
blast CAE61382 Hypothetical protein CBG05229 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004245 EMBL-EBI Protein of unknown function DUF229 9.5E-120
Pfam IPR004245 EMBL-EBI Protein of unknown function DUF229 7.7E-55
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_502870.1 1 13-35
Y73F8A.35 1 13-35
GENEFINDER00000030888 1 13-35
Sequence
Target Sequence 
>C. ELEGANS:184354_AT
atcaatctaccaccaacttcttggacacagaaattctcaaaattccgggagaaaagggat
tttcatttattcggaagcaacctgacacaccgagaaactgtaaaacaatgcccataccaa
ttcaatattgtctttgtaaatttaatagaacaactgaatcaatacacagtgatctcgccc
attcaatggctaaagctctgacagcagctgttaatacggagctggaaaatggaaaacttc
agagtcggtgtataaaaatggagtattcaattattctttctctggatcggttagatgaga
aattccgaggatccatactctacacaattgttgtagaaatgaaaaagcccagtaatgctt
ggtttaaggcaaacatcaaaatgttgagcaccggagagctcaaagtacttggaataattg
aaagagtcaatgaatatggacaaactgctcattgcattaaatctgaatatcatcgacctt
attgtttttgtagaaatcagattaatgtattttatgctcaagataggaaaaagaagagaa
gacagcgagatggatcacctcaaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCAATCTACCACCAACTTCTTGGA 700 25 1433 Antisense
GCAACCTGACACACCGAGAAACTGT 436 319 1509 Antisense
AACAATGCCCATACCAATTCAATAT 55 135 1536 Antisense
TCAATACACAGTGATCTCGCCCATT 538 633 1591 Antisense
GTGATCTCGCCCATTCAATGGCTAA 687 483 1601 Antisense
CAATGGCTAAAGCTCTGACAGCAGC 507 183 1616 Antisense
AATTATTCTTTCTCTGGATCGGTTA 150 173 1701 Antisense
GAAATTCCGAGGATCCATACTCTAC 283 361 1731 Antisense
GGATCCATACTCTACACAATTGTTG 603 511 1741 Antisense
GCACCGGAGAGCTCAAAGTACTTGG 526 315 1820 Antisense
GACAGCGAGATGGATCACCTCAAAA 658 367 1973 Antisense
Sequence Source Affymetrix Proprietary Database