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[184326_at]

Description Y60A3A.10 /REP_DB=WormBase Gene ID /WP=CE24517 /TR=Q9U1Y6 /GB=CAB60405.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 36      4grp_total > 30      6grp_cgc > 25      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 94
1 2 3 4 range
A 81.5
(3)
A 		53.8
(0.7)
A 		8.4
(-3)
A 		70.3
(2.1)
A 		73
B 97.8
(3)
A 		38.3
(-2)
A 		26.7
(-3)
A 		59.4
(-0.2)
A 		71
C 78.7
(3)
A 		4.1
(-3)
A 		30.3
(-0.9)
A 		62.7
(1.7)
A 		75
avr 86
(3) 		32.1
(-2) 		21.8
(-3) 		64.1
(1) 		64

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 72
1 2 3 4 5 6 range
A 79.9
(3)
A 		44.2
(-0.9)
A 		37.6
(-1.6)
A 		48.7
(-0.4)
A 		40.3
(-1.3)
A 		24.5
(-3)
A 		55
B 75.2
(3)
A 		57.6
(1.4)
A 		17.1
(-2.3)
A 		51.1
(0.8)
A 		9.7
(-3)
A 		42.4
(-0)
A 		66
C 8.3
(-3)
A 		65.7
(3)
A 		25.4
(-1.2)
A 		11.7
(-2.6)
A 		20.1
(-1.8)
A 		51.3
(1.5)
A 		57
avr 54.5
(2.8) 		55.8
(3) 		26.7
(-2.4) 		37.2
(-0.4) 		23.4
(-3) 		39.4
(-0) 		32
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 30
1 2 3 4 range
A 29.7
(3)
A 		11.7
(-0.9)
A 		2.2
(-3)
A 		10.3
(-1.2)
A 		28
B 31.2
(3)
A 		9.5
(-2.2)
A 		6.2
(-3)
A 		12.7
(-1.4)
A 		25
C 26.1
(3)
A 		1.2
(-3)
A 		10.6
(-0.7)
A 		10.6
(-0.7)
A 		25
avr 29
(3) 		7.5
(-2.7) 		6.3
(-3) 		11.2
(-1.7) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 14.7
(2.4)
A 		15.7
(3)
A 		8.2
(-1.6)
A 		12.3
(0.9)
A 		12.2
(0.8)
A 		6
(-3)
A 		10
B 26.2
(3)
A 		19.2
(1.1)
A 		4.2
(-2.9)
A 		13.5
(-0.4)
A 		4
(-3)
A 		15.8
(0.2)
A 		22
C 3.1
(-2.8)
A 		20.1
(3)
A 		6.5
(-1.6)
A 		2.4
(-3)
A 		6.5
(-1.6)
A 		17.2
(2)
A 		18
avr 14.7
(1.2) 		18.3
(3) 		6.3
(-3) 		9.4
(-1.5) 		7.6
(-2.4) 		13
(0.4) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184326_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13827
Sequence Type Exemplar sequence
Representative Public ID Y60A3A.10 NCBI
Target Description Y60A3A.10 /REP_DB=WormBase Gene ID /WP=CE24517 /TR=Q9U1Y6 /GB=CAB60405.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075459(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075459 NCBI Caenorhabditis elegans DeHydrogenases, Short chain family member (dhs-24) (dhs-24) mRNA, complete cds. 11/11 None
SNAP00000032801 ENSEMBL cdna:SNAP chromosome:CEL140:V:19940111:19944614:-1 11/11 None
GENEFINDER00000032822 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:19937779:19944614:-1 11/11 None
Y60A3A.10.2 ENSEMBL cdna:known chromosome:CEL140:V:19940111:19944619:-1 gene:Y60A3A.10 11/11 None
Y60A3A.10.1 ENSEMBL cdna:known chromosome:CEL140:V:19940111:19944626:-1 gene:Y60A3A.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:19940110-19944062(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol dhs-24
Ensembl Y60A3A.10
Entrez Gene 180306 Entrez gene
SwissProt Q9U1Y6 EMBL-EBI
WormBase CE38457 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB60405 Hypothetical protein Y60A3A.10 [Caenorhabditis elegans] ref|NP_507860.3| DeHydrogenases, Short chain family member (dhs-24) [Caenorhabditis elegans] 0.0
blast CAE56729 Hypothetical protein CBG24516 [Caenorhabditis briggsae] 1.0E-155
blast CAB60405 Hypothetical protein Y60A3A.10 [Caenorhabditis elegans] ref|NP_507860.3| DeHydrogenases, Short chain family member (dhs-24) [Caenorhabditis elegans] 1.0E-144
blast CAE56729 Hypothetical protein CBG24516 [Caenorhabditis briggsae] 1.0E-137
blast CAE56729 Hypothetical protein CBG24516 [Caenorhabditis briggsae] 1.0E-136
Protein Domains
Database ID Description E-Value
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 6.0E-21
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000032822 1 358-377
Sequence
Target Sequence 
>C. ELEGANS:184326_AT
tcaggcaccaaatacgatcttcacgaggatcttgccggcaaaacgtacatcgtaaccggt
gccacctcaggcatcggccaggcgactgcagaagaactggcaaaacgtaatgcacgtgtc
attatggcttgccggaatcgcgagaaatgcgttcaagttcgccgggatattgtcctgaat
accaggaacaagcaggatggaatcgaaaaaaccatcgccacaaatcacctgggaagcttt
ttgctcaccggactcctcttggacaagctcctcgcccagccgaatcccgtccgaattgta
tttctaaattcgaatattatcgatcgaaagtgtgacctaaatttggcggatttcaactca
gaaaacgccgggaaaaaattcgatggatatgagatttacaagcactcgaaactggcgtcg
gctctttttacgaaagagctctctgagaggctttccgatac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCAGGCACCAAATACGATCTTCACG 36 623 85 Antisense
TCTTCACGAGGATCTTGCCGGCAAA 277 617 102 Antisense
GCATCGGCCAGGCGACTGCAGAAGA 530 307 155 Antisense
GCACGTGTCATTATGGCTTGCCGGA 603 315 196 Antisense
GGCTTGCCGGAATCGCGAGAAATGC 425 537 210 Antisense
AGTTCGCCGGGATATTGTCCTGAAT 189 61 240 Antisense
CCATCGCCACAAATCACCTGGGAAG 380 271 296 Antisense
ACCTGGGAAGCTTTTTGCTCACCGG 415 87 311 Antisense
GAATCCCGTCCGAATTGTATTTCTA 133 329 366 Antisense
TACAAGCACTCGAAACTGGCGTCGG 513 633 481 Antisense
AGCTCTCTGAGAGGCTTTCCGATAC 70 81 521 Antisense
Sequence Source Affymetrix Proprietary Database