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[184324_s_at]

Description C13F10.4 /REP_DB=WormBase Gene ID /WP=CE08145 /TR=O01485 /GB=AAC47965.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 52      6grp_high1_10_10 > 25      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_highest_nonorm > 61      6grp_total > 26      4grp_total > 46      6grp_highest_sub > 01_02      6grp_high1_sub > 01_02      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 276
1 2 3 4 range
A 527.5
(3)
P 		337.8
(-2.4)
P 		316.2
(-3)
P 		322.2
(-2.8)
P 		211
B 592.1
(3)
P 		403.8
(-1.4)
P 		337.9
(-3)
P 		342.2
(-2.9)
P 		254
C 485.5
(3)
P 		425.1
(0.3)
P 		352.2
(-3)
P 		369.3
(-2.2)
P 		133
avr 535
(3) 		388.9
(-1.4) 		335.4
(-3) 		344.6
(-2.7) 		200

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 275
1 2 3 4 5 6 range
A 571
(3)
P 		498
(1.2)
P 		330.2
(-2.8)
P 		321.2
(-3)
P 		424.3
(-0.5)
P 		386.6
(-1.4)
P 		250
B 514.7
(1.2)
P 		595.9
(3)
P 		382.2
(-1.7)
P 		472.6
(0.3)
P 		323.4
(-3)
P 		386.7
(-1.6)
P 		273
C 446
(0.6)
P 		521.5
(3)
P 		342.4
(-2.6)
P 		426.7
(0)
P 		330.5
(-3)
P 		336.8
(-2.8)
P 		191
avr 510.6
(2.1) 		538.5
(3) 		351.6
(-3) 		406.8
(-1.2) 		359.4
(-2.7) 		370
(-2.4) 		187
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 145
1 2 3 4 range
A 192.1
(3)
P 		73.3
(-1.9)
P 		82.7
(-1.5)
P 		47.2
(-3)
P 		145
B 188.8
(3)
P 		99.7
(-1.6)
P 		78.3
(-2.7)
P 		72.9
(-3)
P 		116
C 160.7
(3)
P 		122.2
(0.7)
P 		122.8
(0.7)
P 		62.3
(-3)
P 		98
avr 180.5
(3) 		98.4
(-1.1) 		94.6
(-1.3) 		60.8
(-3) 		120

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 126
1 2 3 4 5 6 range
A 105.3
(-1.1)
P 		177.1
(3)
P 		71.9
(-3)
P 		81.5
(-2.5)
P 		128
(0.2)
P 		94.1
(-1.7)
P 		105
B 179.1
(1.9)
P 		198.3
(3)
P 		92.8
(-3)
P 		125.2
(-1.2)
P 		131.4
(-0.8)
P 		144.2
(-0.1)
P 		106
C 165.3
(3)
P 		159.1
(2.5)
P 		87.1
(-2.9)
P 		85.8
(-3)
P 		106.9
(-1.4)
P 		112.8
(-1)
P 		80
avr 149.9
(1.2) 		178.2
(3) 		83.9
(-3) 		97.5
(-2.1) 		122.1
(-0.6) 		117
(-0.9) 		94
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184324_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11465
Sequence Type Exemplar sequence
Representative Public ID C13F10.4 NCBI
Target Description C13F10.4 /REP_DB=WormBase Gene ID /WP=CE08145 /TR=O01485 /GB=AAC47965.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072427(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072427 NCBI Caenorhabditis elegans C13F10.4 (C13F10.4) mRNA, complete cds. 11/11 None
SNAP00000004757 ENSEMBL cdna:SNAP chromosome:CEL140:V:7208545:7217480:-1 11/11 None
GENEFINDER00000004762 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:7208545:7221582:-1 11/11 None
C13F10.4 ENSEMBL cdna:known chromosome:CEL140:V:7208214:7217480:-1 gene:C13F10.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:7208545-7217481(-) 99.34 100.0
Public Domain and Genome References
Gene Title
Ensembl C13F10.4
Entrez Gene 179109 Entrez gene
SwissProt O01485 EMBL-EBI
WormBase CE08145 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.9986.1.S1_S_AT similar to CG2747-PB, isoform B cfa
DROSOPHILA_2:1629748_S_AT dm
DROSGENOME1:149470_AT dm
CHICKEN:GGA.7454.1.S1_AT similar to KIAA1414 protein gga
HG-U133_PLUS_2:233642_S_AT KIAA1414 protein hs
HG-U133B:233642_S_AT KIAA1414 protein hs
HU35KSUBA:H59417_S_AT KIAA1414 protein hs
HU35KSUBA:D82385_AT KIAA1414 protein hs
HU35KSUBB:RC_N66289_I_AT KIAA1414 protein hs
HU35KSUBD:RC_H73161_F_AT KIAA1414 protein hs
U133_X3P:HS.92991.1.S1_3P_A_AT KIAA1414 protein hs
HG-U95B:55631_AT KIAA1414 protein hs
HG-U95D:89435_AT KIAA1414 protein hs
U133_X3P:HS2.363439.1.S1_3P_X_AT KIAA1414 protein hs
U133_X3P:HS2.363439.1.S1_3P_AT KIAA1414 protein hs
U133_X3P:1569503_3P_AT KIAA1414 protein hs
HG-U133_PLUS_2:1569503_AT KIAA1414 protein hs
HU35KSUBC:RC_H84644_AT KIAA1414 protein hs
MOUSE430A_2:1420449_AT RIKEN cDNA A230048G03 gene mm
MOUSE430_2:1420449_AT RIKEN cDNA A230048G03 gene mm
MOE430A:1420449_AT RIKEN cDNA A230048G03 gene mm
MOUSE430_2:1439815_AT RIKEN cDNA A230048G03 gene mm
MOE430B:1439815_AT RIKEN cDNA A230048G03 gene mm
MG-U74CV2:138396_AT RIKEN cDNA A230048G03 gene mm
MOE430B:1438085_AT RIKEN cDNA A230048G03 gene mm
MOUSE430_2:1438085_AT RIKEN cDNA A230048G03 gene mm
MOE430B:1459634_AT RIKEN cDNA A230048G03 gene mm
MOUSE430_2:1459634_AT RIKEN cDNA A230048G03 gene mm
MOE430B:1459257_AT Premature mRNA for mKIAA1414 protein mm
MOUSE430_2:1459257_AT Premature mRNA for mKIAA1414 protein mm
RAE230B:1383710_AT similar to KIAA1414 protein rn
RAT230_2:1383710_AT similar to KIAA1414 protein rn
Protein Similarities
Method ID Description E-Value
blast AAC47965 Hypothetical protein C13F10.4 [Caenorhabditis elegans] ref|NP_504828.1| C13F10.4 [Caenorhabditis elegans] 0.0
blast CAE72021 Hypothetical protein CBG19103 [Caenorhabditis briggsae] 0.0
blast XP_863392 PREDICTED: similar to CG2747-PB, isoform B isoform 7 [Canis familiaris] 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000004762 1 108-130
Sequence
Target Sequence 
>C. ELEGANS:184324_S_AT
ctcggtcatcaggagtccttcaacaaattaattgtattgctgaagcgtcatttgcaaagt
gataatgtttcagttgtcatgaaaactctacaatcattggcttcgatattcggcagaaaa
ggcttcggtggaattttcgtcaaacatctcggaaaggagattatgccagtagtcaagaga
tatctatcaaaagtggattgtgaaaacgagaagatcacagaatccgacctagtcattctt
caagaatgtgtgaaggttatcgaagttcttgcaatgagcgcaaaagatggaaaacgaata
caagtgatttcgctgcttgttcaacttttggttcgactactcagagcaacatctcataca
gaatggcgaaaagttggtccaattgagaagaagcttcacgagatggcgattggacgacta
aatgcggcagcttccatgtggccagctgaatttaaaaaagttatcgaatggaataatgaa
ttgaagactcgactggagagtgctcttcttcttcaatccaccagacatgctcatcaaatt
tcaatggcaaagactcaagaggcaaagacaacaccggtcgttcagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTCGGTCATCAGGAGTCCTTCAACA 406 231 5614 Antisense
CAATCATTGGCTTCGATATTCGGCA 475 179 5704 Antisense
GTGGAATTTTCGTCAAACATCTCGG 398 491 5741 Antisense
GAATCCGACCTAGTCATTCTTCAAG 260 329 5833 Antisense
AGTGATTTCGCTGCTTGTTCAACTT 52 61 5916 Antisense
AACTTTTGGTTCGACTACTCAGAGC 212 139 5936 Antisense
GGACGACTAAATGCGGCAGCTTCCA 130 523 6025 Antisense
GCTTCCATGTGGCCAGCTGAATTTA 199 307 6043 Antisense
TCGACTGGAGAGTGCTCTTCTTCTT 366 601 6102 Antisense
TTCTTCTTCAATCCACCAGACATGC 58 691 6119 Antisense
GCAAAGACAACACCGGTCGTTCAGC 92 319 6175 Antisense
Sequence Source Affymetrix Proprietary Database