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[184323_at]

Description Y60A3A.14 /REP_DB=WormBase Gene ID /WP=CE24520 /TR=Q9U1Z2 /GB=CAB60399.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 36      6grp_high1_10_10 > 53      4grp_highest_8_8 > 02      4grp_high_8_8 > 46      6grp_highest_nonorm > 90      6grp_total > 52      4grp_total > 49      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 99
1 2 3 4 range
A 201.5
(-3)
P 		238
(2.3)
P 		243
(3)
P 		211.4
(-1.6)
P 		42
B 211.8
(-2)
P 		286.5
(2.5)
P 		294.1
(3)
P 		194.9
(-3)
P 		99
C 208.7
(-3)
P 		281.2
(3)
P 		239.3
(-0.5)
P 		230.9
(-1.2)
P 		73
avr 207.3
(-3) 		268.6
(3) 		258.8
(2) 		212.4
(-2.5) 		61

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 108
1 2 3 4 5 6 range
A 182.3
(-0.7)
P 		166.5
(-1.8)
P 		238.9
(3)
P 		167.5
(-1.7)
P 		148.2
(-3)
P 		190
(-0.2)
P 		91
B 142.4
(-2.2)
P 		177.8
(0)
P 		212.9
(2.2)
P 		225.1
(3)
P 		130.5
(-3)
P 		153.9
(-1.5)
P 		95
C 147.9
(-2.3)
P 		175.4
(0.4)
P 		202.7
(3)
P 		152.9
(-1.8)
P 		140.4
(-3)
P 		146.8
(-2.4)
P 		62
avr 157.5
(-1.6) 		173.2
(-0.4) 		218.2
(3) 		181.8
(0.2) 		139.7
(-3) 		163.6
(-1.2) 		79
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 52
1 2 3 4 range
A 73.4
(3)
P 		51.7
(-0.1)
P 		63.5
(1.6)
P 		31
(-3)
P 		42
B 67.5
(2.3)
P 		70.8
(3)
P 		68.2
(2.5)
P 		41.5
(-3)
P 		29
C 69.1
(1.1)
P 		80.9
(2.7)
P 		83.4
(3)
P 		38.9
(-3)
P 		45
avr 70
(2.7) 		67.8
(2.3) 		71.7
(3) 		37.1
(-3) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 33.6
(-3)
P 		59.2
(3)
P 		52
(1.3)
P 		42.5
(-0.9)
P 		44.7
(-0.4)
P 		46.3
(-0)
P 		26
B 49.6
(-3)
P 		59.2
(2.8)
P 		51.7
(-1.7)
P 		59.6
(3)
P 		53
(-1)
P 		57.4
(1.7)
P 		10
C 54.8
(3)
P 		53.5
(2.7)
P 		51.6
(2.2)
P 		30.7
(-3)
P 		45.4
(0.7)
P 		49.1
(1.6)
P 		24
avr 46
(-2.2) 		57.3
(3) 		51.8
(0.5) 		44.3
(-3) 		47.7
(-1.4) 		50.9
(0) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184323_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13720
Sequence Type Exemplar sequence
Representative Public ID Y60A3A.14 NCBI
Target Description Y60A3A.14 /REP_DB=WormBase Gene ID /WP=CE24520 /TR=Q9U1Z2 /GB=CAB60399.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075458(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075458 NCBI Caenorhabditis elegans Y60A3A.14 (Y60A3A.14) mRNA, complete cds. 11/11 None
SNAP00000032797 ENSEMBL cdna:SNAP chromosome:CEL140:V:19923443:19934188:-1 11/11 None
Y60A3A.14 ENSEMBL cdna:known chromosome:CEL140:V:19925878:19930856:-1 gene:Y60A3A.14 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_075654 5/11 Cross Hyb Matching Probes None
GENEFINDER00000032844 3/11 Cross Hyb Matching Probes None
GENEFINDER00000032846 3/11 Cross Hyb Matching Probes None
SNAP00000032586 3/11 Cross Hyb Matching Probes None
GENEFINDER00000032588 4/11 Cross Hyb Matching Probes None
T08D2.2 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:19925877-19930856(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y60A3A.14
Entrez Gene 190436 Entrez gene
SwissProt Q9U1Z2 EMBL-EBI
WormBase CE24520 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:251626_AT UDP-GlcNAc:dolichol phosphate N-acetylglucosamine-1-phosphate transferase, putative at
CANINE_2:CFA.16867.1.S1_AT similar to UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (GPT) (G1PT) (N-acetylglucosamine-1-phosphate transferase) (GlcNAc-1-P transferase) cfa
CANINE_2:CFAAFFX.19107.1.S1_AT similar to UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (GPT) (G1PT) (N-acetylglucosamine-1-phosphate transferase) (GlcNAc-1-P transferase) cfa
DROSOPHILA_2:1638344_AT dm
DROSGENOME1:152751_AT dm
CHICKEN:GGA.9701.1.S1_AT dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) gga
CHICKEN:GGA.9701.2.S1_A_AT dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) gga
HU35KSUBA:Z82022_AT dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) hs
HG-U133A:209509_S_AT dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) hs
HG-U133_PLUS_2:209509_S_AT dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) hs
HG-FOCUS:209509_S_AT dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) hs
HG-U133A_2:209509_S_AT dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) hs
U133_X3P:G12653116_3P_A_AT dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) hs
HG-U95AV2:35176_AT dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) hs
MU11KSUBB:X65603_S_AT dolichyl-phosphate (UDP-N-acetylglucosamine) acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) mm
MG-U74AV2:103352_AT dolichyl-phosphate (UDP-N-acetylglucosamine) acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) mm
MOE430A:1448549_A_AT dolichyl-phosphate (UDP-N-acetylglucosamine) acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) mm
MOUSE430A_2:1448549_A_AT dolichyl-phosphate (UDP-N-acetylglucosamine) acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) mm
MOUSE430_2:1448549_A_AT dolichyl-phosphate (UDP-N-acetylglucosamine) acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) mm
MG-U74AV2:161420_R_AT Dolichyl-phosphate (UDP-N-acetylglucosamine) acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase), mRNA (cDNA clone MGC:11562 IMAGE:3965178) mm
MU19KSUBA:TC20671_S_AT Dolichyl-phosphate (UDP-N-acetylglucosamine) acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase), mRNA (cDNA clone MGC:11562 IMAGE:3965178) mm
RG-U34B:RC_AA963476_AT dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) rn
RAE230A:1372270_AT dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) rn
RAT230_2:1372270_AT dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) rn
RAE230B:1398147_AT dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) rn
RAT230_2:1398147_AT dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) rn
YEAST_2:1773123_AT UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6629 lipid metabolism inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB60399 Hypothetical protein Y60A3A.14 [Caenorhabditis elegans] ref|NP_507859.1| Y60A3A.14 [Caenorhabditis elegans] 0.0
blast CAE56689 Hypothetical protein CBG24468 [Caenorhabditis briggsae] 1.0E-160
blast CAE56689 Hypothetical protein CBG24468 [Caenorhabditis briggsae] 1.0E-156
blast CAE56728 Hypothetical protein CBG24515 [Caenorhabditis briggsae] 1.0E-141
blast CAE56728 Hypothetical protein CBG24515 [Caenorhabditis briggsae] 1.0E-136
Protein Domains
Database ID Description E-Value
Pfam IPR000715 EMBL-EBI Glycosyl transferase, family 4 2.2E-59
Pfam IPR000715 EMBL-EBI Glycosyl transferase, family 4 2.2E-59
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507859.1 9 2-21,36-53,74-96,111-133,145-164,174-193,200-222,227-249,334-356
Y60A3A.14 9 2-21,36-53,74-96,111-133,145-164,174-193,200-222,227-249,334-356
SNAP00000032797 9 63-85,98-117,137-159,166-188,234-256,263-285,290-312,397-419,424-443
Sequence
Target Sequence 
>C. ELEGANS:184323_AT
atttcgtgcagattttccgtttctctgctgaaaatagtactggattctggcatcatacca
tctcgctctactttttgctaccatttactgcgtgtacagcgatactattttattttaata
agtacccatctcgtgtatttgtcggtgatacattctgctactggtccggcatgacacttg
cagttgtatcgattttgggacacttcagcaagactctgatgctcttcttcgttccacaaa
ttatcaatttcctctattcaattcctcaacttttccatttggtaccgtgtccacgtcatc
gactaccgaaatatgacccgaaaactgatacagtcagtatgagcatcgctgaattcaaga
aaaccgatttgaaacgacttggagcactttttattgcagtctgcaagtctattggaatgc
ttcatgtaaaagaagtggagaaggatggggaaatctatctccaaatcaacaatctgacaa
tcatcaatttggtgctgaaattcgccggaccccttcacgagaagaccctcaatgatgtgc
taatgagcattcaaattctgtgctcattactcgcattc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTTCGTGCAGATTTTCCGTTTCTC 443 17 476 Antisense
TGGATTCTGGCATCATACCATCTCG 225 551 516 Antisense
TACTTTTTGCTACCATTTACTGCGT 258 645 544 Antisense
GTACCCATCTCGTGTATTTGTCGGT 59 459 597 Antisense
TCGGTGATACATTCTGCTACTGGTC 569 605 617 Antisense
GTCCGGCATGACACTTGCAGTTGTA 131 477 639 Antisense
AGCAAGACTCTGATGCTCTTCTTCG 207 77 682 Antisense
GCTCTTCTTCGTTCCACAAATTATC 267 301 696 Antisense
CATTTGGTACCGTGTCCACGTCATC 450 209 751 Antisense
TTTGGTGCTGAAATTCGCCGGACCC 232 667 963 Antisense
AATTCTGTGCTCATTACTCGCATTC 477 177 1029 Antisense
Sequence Source Affymetrix Proprietary Database