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[184288_at]

Description H14A12.3 /REP_DB=WormBase Gene ID /WP=CE11582 /TR=O17213 /GB=AAB70984.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 94      6grp_high1_10_10 > 59      4grp_highest_8_8 > 56      4grp_high_8_8 > 35      6grp_highest_nonorm > 75      6grp_total > 49      4grp_total > 44      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 363
1 2 3 4 range
A 892.6
(1.9)
P 		953.4
(3)
P 		673.3
(-2.1)
P 		625.6
(-3)
P 		328
B 777.4
(0.6)
P 		858.5
(3)
P 		653.3
(-3)
P 		712.7
(-1.3)
P 		205
C 988.5
(3)
P 		860.7
(0.8)
P 		761.2
(-0.9)
P 		637.1
(-3)
P 		351
avr 886.2
(2.9) 		890.9
(3) 		695.9
(-2) 		658.5
(-3) 		232

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 406
1 2 3 4 5 6 range
A 822.4
(-0.4)
P 		1033
(3)
P 		663
(-3)
P 		796.3
(-0.8)
P 		767.6
(-1.3)
P 		872.1
(0.4)
P 		370
B 826.6
(2.1)
P 		776.4
(0.8)
P 		626.7
(-3)
P 		860.6
(3)
P 		732.1
(-0.3)
P 		784.6
(1.1)
P 		234
C 893
(1)
P 		709
(-3)
P 		974.9
(2.8)
P 		788.7
(-1.3)
P 		984.8
(3)
P 		943.8
(2.1)
P 		276
avr 847.3
(2) 		839.5
(1.5) 		754.9
(-3) 		815.2
(0.2) 		828.2
(0.9) 		866.8
(3) 		112
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 236
1 2 3 4 range
A 325
(3)
P 		206.9
(-0)
P 		176
(-0.8)
P 		91.6
(-3)
P 		233
B 247.9
(3)
P 		212
(0.8)
P 		151.4
(-3)
P 		151.9
(-3)
P 		97
C 327.2
(3)
P 		247.5
(0.8)
P 		265.3
(1.3)
P 		107.4
(-3)
P 		220
avr 300
(3) 		222.1
(0.4) 		197.6
(-0.4) 		117
(-3) 		183

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 223
1 2 3 4 5 6 range
A 151.6
(-2.8)
P 		367.4
(3)
P 		144.4
(-3)
P 		202
(-1.5)
P 		231.6
(-0.7)
P 		212.3
(-1.2)
P 		223
B 287.6
(2.6)
P 		258.4
(1.4)
P 		152.2
(-3)
P 		227.9
(0.1)
P 		297.5
(3)
P 		292.6
(2.8)
P 		145
C 331
(3)
P 		216.3
(-1)
P 		248.1
(0.1)
P 		158.6
(-3)
P 		318.7
(2.6)
P 		316
(2.5)
P 		172
avr 256.7
(1.5) 		280.7
(2.9) 		181.6
(-3) 		196.2
(-2.1) 		282.6
(3) 		273.6
(2.5) 		101
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184288_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6770
Sequence Type Exemplar sequence
Representative Public ID H14A12.3 NCBI
Target Description H14A12.3 /REP_DB=WormBase Gene ID /WP=CE11582 /TR=O17213 /GB=AAB70984.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066240(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066240 NCBI Caenorhabditis elegans H14A12.3 (H14A12.3) mRNA, complete cds. 11/11 None
SNAP00000033734 ENSEMBL cdna:SNAP chromosome:CEL140:III:7463843:7464942:1 11/11 None
GENEFINDER00000033744 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7463843:7464942:1 11/11 None
H14A12.3 ENSEMBL cdna:known chromosome:CEL140:III:7463733:7465089:1 gene:H14A12.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7463704-7464940(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl H14A12.3
Entrez Gene 176058 Entrez gene
SwissProt O17213 EMBL-EBI
WormBase CE28734 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB70984 Hypothetical protein H14A12.3 [Caenorhabditis elegans] ref|NP_498641.1| H14A12.3 [Caenorhabditis elegans] 1.0E-166
blast CAE64465 Hypothetical protein CBG09181 [Caenorhabditis briggsae] 1.0E-140
blast AAF49370 CG7725-PA, isoform A [Drosophila melanogaster] gb|AAL25418.1| LD26833p [Drosophila melanogaster] ref|NP_648956.1| CG7725-PA, isoform A [Drosophila melanogaster] 6.0E-19
Sequence
Target Sequence 
>C. ELEGANS:184288_AT
gaaggcagcagtcacacttctggacgacaatgttattcaagctgaagtgaccgtaaagta
tcctaaagctggtggtggatattatcgagcagtcgcacaacccgacgtccaatggaaact
tcaacaacttcaggatcttggaaatcacatttccagagttacaatcactctctgcgattt
acaacatgaagttaatttgttgaaaggagatggagaacgagatgctttcactctggctac
cggagcacgtattttggaggaactcaagctcaccatgaatgaaatatcattggctcgaaa
ttcaattatgttacctagaaaaagaagcctgttggagttgtgttacttcccaccgacccg
gaaatttgttccacctcttcctcaagatcaactaatctccttctatatttcatgttgccg
cttggtttgtgcatcctaccaaatggttccgaagacagttcatccacaaggcctctcagt
gttcatggcagaatctcaacttccacacctcgacgatgtcatcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGGCAGCAGTCACACTTCTGGAC 651 333 276 Antisense
GTTACAATCACTCTCTGCGATTTAC 700 445 433 Antisense
AGAACGAGATGCTTTCACTCTGGCT 669 73 489 Antisense
CTCTGGCTACCGGAGCACGTATTTT 245 231 506 Antisense
TGTTGGAGTTGTGTTACTTCCCACC 578 559 605 Antisense
GATCAACTAATCTCCTTCTATATTT 664 421 661 Antisense
TTTCATGTTGCCGCTTGGTTTGTGC 185 671 683 Antisense
GAAGACAGTTCATCCACAAGGCCTC 281 339 726 Antisense
CAAGGCCTCTCAGTGTTCATGGCAG 173 183 742 Antisense
CATGGCAGAATCTCAACTTCCACAC 484 209 759 Antisense
TTCCACACCTCGACGATGTCATCAA 597 699 776 Antisense
Sequence Source Affymetrix Proprietary Database