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[184284_s_at]

Description Y51H4A.25 /REP_DB=WormBase Gene ID /WP=CE25368 /TR=Q9TVP0 /GB=CAB63368.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 12      6grp_total > 30      4grp_total > 14      6grp_high1_sub > De      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 98
1 2 3 4 range
A 51.6
(-1.4)
P 		32.7
(-3)
P 		41.2
(-2.3)
A 		103.1
(3)
A 		70
B 99.9
(3)
P 		80.2
(1.6)
P 		17.3
(-3)
A 		88.1
(2.1)
P 		83
C 81.5
(1.7)
P 		5.4
(-3)
A 		30.8
(-1.4)
A 		103.4
(3)
P 		98
avr 77.7
(1.2) 		39.4
(-2.2) 		29.8
(-3) 		98.2
(3) 		68

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 69
1 2 3 4 5 6 range
A 28.6
(-2.6)
A 		43.6
(-1)
P 		82.6
(3)
P 		64.8
(1.2)
P 		59
(0.6)
P 		24.3
(-3)
A 		58
B 54.7
(3)
A 		51.8
(2.4)
P 		54.7
(3)
A 		44.5
(0.8)
P 		27
(-3)
P 		40.6
(-0.1)
P 		28
C 64.6
(0.4)
P 		93.7
(3)
P 		47.7
(-1.2)
P 		51.4
(-0.8)
A 		38.8
(-2)
P 		27.3
(-3)
A 		66
avr 49.3
(0.5) 		63
(3) 		61.7
(2.8) 		53.6
(1.3) 		41.6
(-1) 		30.7
(-3) 		32
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 30
1 2 3 4 range
A 18.8
(3)
P 		7.1
(-3)
P 		10.8
(-1.1)
A 		15.1
(1.1)
A 		12
B 31.8
(3)
P 		19.8
(0.4)
P 		4
(-3)
A 		18.8
(0.2)
P 		28
C 27
(3)
P 		1.5
(-3)
A 		10.7
(-0.8)
A 		17.4
(0.7)
P 		26
avr 25.9
(3) 		9.5
(-2.7) 		8.5
(-3) 		17.1
(-0) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 23
1 2 3 4 5 6 range
A 5.3
(-3)
A 		15.5
(1.8)
P 		18
(3)
P 		16.4
(2.2)
P 		17.8
(2.9)
P 		5.9
(-2.7)
A 		13
B 19
(3)
A 		17.3
(1.7)
P 		13.3
(-1.3)
A 		11.8
(-2.4)
P 		11
(-3)
P 		15.1
(0.1)
P 		8
C 23.9
(1.6)
P 		28.6
(3)
P 		12.1
(-2.1)
P 		10.3
(-2.6)
A 		12.6
(-1.9)
P 		9.1
(-3)
A 		20
avr 16.1
(0.5) 		20.5
(3) 		14.5
(-0.4) 		12.8
(-1.4) 		13.8
(-0.8) 		10
(-3) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184284_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8389
Sequence Type Exemplar sequence
Representative Public ID Y51H4A.25 NCBI
Target Description Y51H4A.25 /REP_DB=WormBase Gene ID /WP=CE25368 /TR=Q9TVP0 /GB=CAB63368.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028310(11), NM_070675(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028310 NCBI Caenorhabditis elegans Y51H4A.25a (Y51H4A.25) mRNA, complete cds. 11/11 None
NM_070675 NCBI Caenorhabditis elegans T28F3.9 (T28F3.9) mRNA, complete cds. 11/11 A 
SNAP00000037215 ENSEMBL cdna:SNAP chromosome:CEL140:IV:16741384:16743040:1 11/11 None
GENEFINDER00000037226 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:16741384:16743040:1 11/11 None
Y51H4A.25a ENSEMBL cdna:known chromosome:CEL140:IV:16736288:16743047:1 gene:Y51H4A.25 11/11 A 
T28F3.9 ENSEMBL cdna:known chromosome:CEL140:IV:17308480:17311650:1 gene:T28F3.9 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000040190 4/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
190159 2 1 NM_001028310 184283_at (11), 184284_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:16740136-16743046(+) 100.0 100.0
chrIV:17308485-17311656(+) 95.78 95.78
Public Domain and Genome References
Gene Title
Ensembl Y51H4A.25
T28F3.9
Entrez Gene 189057 Entrez gene
SwissProt Q9XTM4 EMBL-EBI
WormBase CE20119 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8375 acetylglucosaminyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB63388 Hypothetical protein Y51H4A.25a [Caenorhabditis elegans] emb|CAB63368.1| Hypothetical protein Y51H4A.25a [Caenorhabditis elegans] ref|NP_001023481.1| Y51H4A.25a [Caenorhabditis elegans] 0.0
blast CAB05303 Hypothetical protein T28F3.9 [Caenorhabditis elegans] emb|CAB05168.1| Hypothetical protein T28F3.9 [Caenorhabditis elegans] ref|NP_503076.1| T28F3.9 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003406 EMBL-EBI Glycosyl transferase, family 14 4.7E-78
Pfam IPR003406 EMBL-EBI Glycosyl transferase, family 14 5.3E-75
Pfam IPR003406 EMBL-EBI Glycosyl transferase, family 14 4.7E-78
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001023481.1 1 46-68
NP_503076.1 1 45-67
Y51H4A.25a 1 46-68
T28F3.9 1 45-67
Sequence
Target Sequence 
>C. ELEGANS:184284_S_AT
gtttcgaaacgaatcgggactcgatgatgaacttttgaagaaaccaatgaaaatagccag
tggatatgtacaagcttcattgtcaagagcagctgttgaatggttaatcaatacagtgga
tttaacattattcatcaaccagtttgatcgaacgaaatatggtggagacgagcagctgat
ttccagttttcaagtgaattcacaatttgaaatgcctggacactttacggacgagtgttc
caaatatcgttacgggcatgatcaaatctcaagaatgactcaatgggctggtggtgatgt
aaaaaggtgtgcctcaaaaaccatacgtcatgggatttgtctcatcggaattgaagattt
cagagctgtttctgaaatgccagttattatgttcaataagatgttgccatcgtttgacta
ctccataattgattgcacagctgaacttcttttcaatcgaacgttcctgaatcagacaaa
tattaaattaaatgaaaagtactatgaagaaatggttacggttatttatcacaaagatca
caaaaatccgggttatcatctgaattgcaccccca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTTCGAAACGAATCGGGACTCGAT 705 447 945 Antisense
GGGACTCGATGATGAACTTTTGAAG 512 493 960 Antisense
AAGAGCAGCTGTTGAATGGTTAATC 71 151 1029 Antisense
TAACATTATTCATCAACCAGTTTGA 560 639 1067 Antisense
GGAGACGAGCAGCTGATTTCCAGTT 307 519 1108 Antisense
GCTGATTTCCAGTTTTCAAGTGAAT 158 295 1119 Antisense
CACAATTTGAAATGCCTGGACACTT 381 193 1145 Antisense
AAACCATACGTCATGGGATTTGTCT 452 125 1262 Antisense
ATGGTTACGGTTATTTATCACAAAG 342 49 1456 Antisense
GATCACAAAAATCCGGGTTATCATC 616 421 1480 Antisense
GGTTATCATCTGAATTGCACCCCCA 70 507 1495 Antisense
Sequence Source Affymetrix Proprietary Database