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[184274_at]

Description Y32B12B.4 /REP_DB=WormBase Gene ID /WP=CE24197 /TR=Q9XX31 /GB=CAA21009.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 88      6grp_high1_10_10 > 88      4grp_highest_8_8 > 0      4grp_high_8_8 > 36      6grp_highest_nonorm > 91      6grp_total > 98      4grp_total > 43      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 98
1 2 3 4 range
A 180.8
(-0.2)
P 		193.1
(3)
P 		191.7
(2.6)
P 		169.9
(-3)
P 		23
B 197.3
(1.2)
P 		219.4
(3)
P 		164.1
(-1.6)
P 		146.5
(-3)
P 		73
C 156
(-3)
P 		244.7
(3)
P 		174.2
(-1.8)
P 		203.9
(0.2)
P 		89
avr 178
(-2.4) 		219.1
(3) 		176.7
(-2.6) 		173.4
(-3) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 204
1 2 3 4 5 6 range
A 206.7
(-1.6)
P 		196.1
(-2)
P 		163.4
(-3)
P 		192.7
(-2.1)
P 		289.9
(1)
P 		355.2
(3)
P 		192
B 197
(-1)
P 		200.2
(-0.8)
P 		166.5
(-3)
P 		230.1
(1.1)
P 		225.3
(0.8)
P 		260.1
(3)
P 		94
C 151.7
(-3)
P 		183.3
(-1.5)
P 		184.9
(-1.4)
P 		176.4
(-1.8)
P 		250.9
(1.7)
P 		279.1
(3)
P 		127
avr 185.1
(-2.4) 		193.2
(-2) 		171.6
(-3) 		199.7
(-1.7) 		255.4
(1) 		298.1
(3) 		127
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 45
1 2 3 4 range
A 65.8
(3)
P 		41.9
(-0.5)
P 		50.1
(0.7)
P 		24.9
(-3)
P 		41
B 62.9
(3)
P 		54.2
(1.4)
P 		38
(-1.7)
P 		31.2
(-3)
P 		32
C 51.6
(-0.1)
P 		70.3
(3)
P 		60.7
(1.4)
P 		34.4
(-3)
P 		36
avr 60.1
(3) 		55.5
(2.1) 		49.6
(0.9) 		30.2
(-3) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 62
1 2 3 4 5 6 range
A 38.1
(-2.7)
P 		69.7
(0.9)
P 		35.6
(-3)
P 		48.9
(-1.5)
P 		87.5
(3)
P 		86.5
(2.9)
P 		52
B 68.5
(-0)
P 		66.6
(-0.2)
P 		40.4
(-3)
P 		60.9
(-0.8)
P 		91.5
(2.4)
P 		97
(3)
P 		57
C 56.2
(-0.9)
P 		55.9
(-0.9)
P 		47.1
(-1.8)
P 		35.5
(-3)
P 		81.2
(1.7)
P 		93.4
(3)
P 		58
avr 54.3
(-1.4) 		64.1
(-0.3) 		41
(-3) 		48.4
(-2.1) 		86.7
(2.3) 		92.3
(3) 		51
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184274_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14745
Sequence Type Exemplar sequence
Representative Public ID Y32B12B.4 NCBI
Target Description Y32B12B.4 /REP_DB=WormBase Gene ID /WP=CE24197 /TR=Q9XX31 /GB=CAA21009.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074789(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074789 NCBI Caenorhabditis elegans Y32B12B.4 (Y32B12B.4) mRNA, complete cds. 11/11 None
SNAP00000012872 ENSEMBL cdna:SNAP chromosome:CEL140:V:16555917:16566719:1 10/11 None
Y32B12B.4 ENSEMBL cdna:known chromosome:CEL140:V:16555917:16566073:1 gene:Y32B12B.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000012876 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16555916-16565374(+) 98.17 99.91
Public Domain and Genome References
Gene Title
Ensembl Y32B12B.4
Entrez Gene 180110 Entrez gene
SwissProt Q9XX31 EMBL-EBI
WormBase CE24197 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA21009 Hypothetical protein Y32B12B.4 [Caenorhabditis elegans] ref|NP_507190.1| Y32B12B.4 [Caenorhabditis elegans] 0.0
Sequence
Target Sequence 
>C. ELEGANS:184274_AT
gagatcggaaagccttccagcggtcattcaggcagctccagatgctccagaagcccatcc
aaagcgaatcaagactgaaccacttgtggctccaccacaggagaagccttcaacatcaga
agttcgggatcatgcgccttgtttgaaaatggagatcgtggatcgggttttagaaccacc
ggcagacttaccatcggaaaaaataccggaaactgctgctccgcgaaaaaaactgaatct
caacgcgtcatgctgtgtctgcaaacggctttttcccatcaaagacctcaactatttccg
atttgaaaacgataaaatgatcatggttctaggacaagctctacgtggaaattttttgga
tttgaaaaaattccgaactctccgagccatatatgcctgtgatatgcatgtttctcaggg
gatcagtgagattttcaaatgtcttcaaatcactgatcacacgggcatcgccacgtgttt
tacgcattccatcgagaatatgttcgcctcactcggatcaccgttcccggaatttgtaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGATCGGAAAGCCTTCCAGCGGTC 688 395 4023 Antisense
AGACTGAACCACTTGTGGCTCCACC 405 69 4094 Antisense
TCAGAAGTTCGGGATCATGCGCCTT 415 621 4138 Antisense
TTAGAACCACCGGCAGACTTACCAT 292 681 4192 Antisense
AAATACCGGAAACTGCTGCTCCGCG 177 117 4223 Antisense
AATCTCAACGCGTCATGCTGTGTCT 339 169 4258 Antisense
TGCTGTGTCTGCAAACGGCTTTTTC 579 581 4273 Antisense
CTCTCCGAGCCATATATGCCTGTGA 491 231 4400 Antisense
ACACGGGCATCGCCACGTGTTTTAC 542 101 4481 Antisense
ACGTGTTTTACGCATTCCATCGAGA 32 95 4495 Antisense
GATCACCGTTCCCGGAATTTGTACA 476 419 4538 Antisense
Sequence Source Affymetrix Proprietary Database