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[184229_at]

Description Y37D8A.10 /REP_DB=WormBase Gene ID /WP=CE20214 /TR=Q9XWW1 /GB=CAA21529.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 38      6grp_high1_10_10 > 72      4grp_highest_8_8 > 11      4grp_high_8_8 > 55      6grp_highest_nonorm > 57      6grp_total > 72      4grp_total > 56      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2113
1 2 3 4 range
A 1811.4
(-3)
P 		2735.4
(-0.3)
P 		3868.9
(3)
P 		1894.4
(-2.8)
P 		2058
B 1821.2
(-3)
P 		2908.8
(0.4)
P 		3730.1
(3)
P 		2150
(-2)
P 		1909
C 1810.6
(-2.8)
P 		2983.9
(1.1)
P 		3565.7
(3)
P 		1755.6
(-3)
P 		1810
avr 1814.4
(-3) 		2876
(0.3) 		3721.6
(3) 		1933.3
(-2.6) 		1907

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 998
1 2 3 4 5 6 range
A 1882
(-3)
P 		2264.3
(-0.7)
P 		2481.9
(0.6)
P 		2880.1
(3)
P 		2301.1
(-0.5)
P 		2181.8
(-1.2)
P 		998
B 2163.7
(-2.2)
P 		2067.7
(-3)
P 		2761.5
(3)
P 		2457.3
(0.4)
P 		2619.6
(1.8)
P 		2220.2
(-1.7)
P 		694
C 2337
(0.1)
P 		1963.5
(-3)
P 		2597.2
(2.2)
P 		2696.7
(3)
P 		2520.9
(1.6)
P 		2424.3
(0.8)
P 		733
avr 2127.6
(-2.7) 		2098.5
(-3) 		2613.5
(2.3) 		2678
(3) 		2480.5
(1) 		2275.4
(-1.2) 		580
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 966
1 2 3 4 range
A 659.5
(0.1)
P 		593.8
(-0.4)
P 		1011.4
(3)
P 		277.3
(-3)
P 		734
B 580.8
(-1.2)
P 		718.4
(0.8)
P 		864.3
(3)
P 		458.2
(-3)
P 		406
C 599.4
(-1.1)
P 		857.9
(0.6)
P 		1242.8
(3)
P 		296
(-3)
P 		947
avr 613.2
(-0.7) 		723.4
(0.3) 		1039.5
(3) 		343.8
(-3) 		696

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 717
1 2 3 4 5 6 range
A 346.9
(-3)
P 		805.3
(3)
P 		540.5
(-0.5)
P 		730.5
(2)
P 		694.4
(1.5)
P 		531.2
(-0.6)
P 		458
B 752.9
(-1.5)
P 		688.3
(-2.5)
P 		670.6
(-2.7)
P 		650.9
(-3)
P 		1064.3
(3)
P 		827.9
(-0.4)
P 		413
C 866.2
(3)
P 		599.1
(-1.9)
P 		661
(-0.8)
P 		542.3
(-3)
P 		815.7
(2.1)
P 		811.6
(2)
P 		324
avr 655.3
(-2.2) 		697.6
(-1.1) 		624
(-3) 		641.2
(-2.6) 		858.1
(3) 		723.6
(-0.4) 		234
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184229_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6386
Sequence Type Exemplar sequence
Representative Public ID Y37D8A.10 NCBI
Target Description Y37D8A.10 /REP_DB=WormBase Gene ID /WP=CE20214 /TR=Q9XWW1 /GB=CAA21529.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067275(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067275 NCBI Caenorhabditis elegans Y37D8A.10 (Y37D8A.10) mRNA, complete cds. 11/11 None
SNAP00000028778 ENSEMBL cdna:SNAP chromosome:CEL140:III:12874904:12875500:1 11/11 None
GENEFINDER00000028815 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:12874904:12875500:1 11/11 None
Y37D8A.10 ENSEMBL cdna:known chromosome:CEL140:III:12874902:12875656:1 gene:Y37D8A.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:12874899-12875496(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y37D8A.10
Entrez Gene 176703 Entrez gene
SwissProt Q9XWW1 EMBL-EBI
WormBase CE20214 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1582672_S_AT signal peptidase complex subunit 2 homolog (S. cerevisiae) cfa
CANINE:1592832_S_AT signal peptidase complex subunit 2 homolog (S. cerevisiae) cfa
CANINE_2:CFA.1257.1.A1_S_AT signal peptidase complex subunit 2 homolog (S. cerevisiae) cfa
CANINE_2:CFA.1257.1.A2_AT signal peptidase complex subunit 2 homolog (S. cerevisiae) cfa
CANINE:1592833_AT Signal peptidase complex 25 kDa subunit cfa
CANINE:1592834_S_AT Signal peptidase complex 25 kDa subunit cfa
CANINE:1594753_S_AT Signal peptidase complex 25 kDa subunit cfa
DROSOPHILA_2:1637839_AT dm
DROSGENOME1:154977_AT dm
CHICKEN:GGA.8045.2.S1_A_AT signal peptidase complex subunit 2 homolog (S. cerevisiae) gga
HG-U133_PLUS_2:201239_S_AT signal peptidase complex subunit 2 homolog (S. cerevisiae) hs
HG-U133A_2:201239_S_AT signal peptidase complex subunit 2 homolog (S. cerevisiae) hs
HG-U133A:201239_S_AT signal peptidase complex subunit 2 homolog (S. cerevisiae) hs
HG-U133_PLUS_2:201240_S_AT signal peptidase complex subunit 2 homolog (S. cerevisiae) hs
HG-U133A_2:201240_S_AT signal peptidase complex subunit 2 homolog (S. cerevisiae) hs
HG-U133A:201240_S_AT signal peptidase complex subunit 2 homolog (S. cerevisiae) hs
HUGENEFL:D14658_AT signal peptidase complex subunit 2 homolog (S. cerevisiae) hs
U133_X3P:G7661907_3P_A_AT signal peptidase complex subunit 2 homolog (S. cerevisiae) hs
U133_X3P:HS.77665.0.S1_3P_A_AT signal peptidase complex subunit 2 homolog (S. cerevisiae) hs
HG-U95AV2:37359_AT signal peptidase complex subunit 2 homolog (S. cerevisiae) hs
HG-U95E:70601_R_AT signal peptidase complex subunit 2 homolog (S. cerevisiae) hs
HG-U133_PLUS_2:243570_AT Signal peptidase complex subunit 2 homolog (S. cerevisiae) hs
HG-U133B:243570_AT Signal peptidase complex subunit 2 homolog (S. cerevisiae) hs
HG-U95B:48277_AT Signal peptidase complex subunit 2 homolog (S. cerevisiae) hs
U133_X3P:HS.87095.0.A1_3P_AT Signal peptidase complex subunit 2 homolog (S. cerevisiae) hs
HU35KSUBB:RC_AA232885_AT Signal peptidase complex subunit 2 homolog (S. cerevisiae) hs
MU11KSUBA:AA109790_G_AT signal peptidase complex subunit 2 homolog (S. cerevisiae) mm
MU11KSUBA:AA184359_S_AT signal peptidase complex subunit 2 homolog (S. cerevisiae) mm
MU11KSUBA:AA109790_AT signal peptidase complex subunit 2 homolog (S. cerevisiae) mm
MG-U74AV2:96855_AT signal peptidase complex subunit 2 homolog (S. cerevisiae) mm
MOE430A:1423215_AT signal peptidase complex subunit 2 homolog (S. cerevisiae) mm
MOUSE430A_2:1423215_AT signal peptidase complex subunit 2 homolog (S. cerevisiae) mm
MOUSE430_2:1423215_AT signal peptidase complex subunit 2 homolog (S. cerevisiae) mm
MOE430A:1450907_AT signal peptidase complex subunit 2 homolog (S. cerevisiae) mm
MOUSE430A_2:1450907_AT signal peptidase complex subunit 2 homolog (S. cerevisiae) mm
MOUSE430_2:1450907_AT signal peptidase complex subunit 2 homolog (S. cerevisiae) mm
RG-U34B:RC_AA943550_AT similar to RIKEN cDNA 5730406I15 (predicted) rn
RG-U34C:AA942693_AT similar to RIKEN cDNA 5730406I15 (predicted) rn
RAE230A:1389140_AT similar to RIKEN cDNA 5730406I15 (predicted) rn
RAT230_2:1389140_AT similar to RIKEN cDNA 5730406I15 (predicted) rn
RG-U34B:RC_AA997014_AT Similar to RIKEN cDNA 5730406I15 (predicted) rn
RG-U34C:RC_AI228820_AT Similar to RIKEN cDNA 5730406I15 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast CAA21529 Hypothetical protein Y37D8A.10 [Caenorhabditis elegans] ref|NP_499676.1| Y37D8A.10 [Caenorhabditis elegans] sp|Q9XWW1|SPCS2_CAEEL Probable signal peptidase complex subunit 2 (Microsomal signal peptidase 25 kDa subunit) (SPase 25 kDa subunit) 1.0E-102
blast CAA21529 Hypothetical protein Y37D8A.10 [Caenorhabditis elegans] ref|NP_499676.1| Y37D8A.10 [Caenorhabditis elegans] sp|Q9XWW1|SPCS2_CAEEL Probable signal peptidase complex subunit 2 (Microsomal signal peptidase 25 kDa subunit) (SPase 25 kDa subunit) 1.0E-98
blast Q615A2 Probable signal peptidase complex subunit 2 (Microsomal signal peptidase 25 kDa subunit) (SPase 25 kDa subunit) emb|CAE69555.1| Hypothetical protein CBG15767 [Caenorhabditis briggsae] 9.0E-91
blast Q615A2 Probable signal peptidase complex subunit 2 (Microsomal signal peptidase 25 kDa subunit) (SPase 25 kDa subunit) emb|CAE69555.1| Hypothetical protein CBG15767 [Caenorhabditis briggsae] 3.0E-87
blast NP_001013487 hypothetical protein LOC541342 [Danio rerio] gb|AAH91463.1| Zgc:110364 [Danio rerio] sp|Q5BJI9|SPCS2_BRARE Probable signal peptidase complex subunit 2 (Microsomal signal peptidase 25 kDa subunit) (SPase 25 kDa subunit) 6.0E-30
Protein Domains
Database ID Description E-Value
Pfam IPR009582 EMBL-EBI Microsomal signal peptidase 25 kDa subunit 3.4E-118
Pfam IPR009582 EMBL-EBI Microsomal signal peptidase 25 kDa subunit 3.4E-111
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499676.1 2 41-58,73-95
Y37D8A.10 2 41-58,73-95
SNAP00000028778 2 41-58,73-95
GENEFINDER00000028815 2 56-73,88-110
Sequence
Target Sequence 
>C. ELEGANS:184229_AT
gacggtccaacagtgaagaatgcccttgatgaagtcgtcaaaaagattctgaacgacaaa
gtcggatggacggaatcgcataatctaatgaatctgcgtcttctcatctcattcatcggc
gtcgcgttctccgcgttcgcctgcggctacgactactacgagccgttcccaaagtcgaaa
atcgtgctcgccgtgtgctcggtttcctacttcatatgcatgggaattcttcaaatgtat
cagtggtacgtcgagaaggactgcatctacgaggccaccgaagtcgacggaaaacaatcg
agaaaatgggcgtggagctcggagatcaaggctcacgacgataagtacacgctgtcggcc
gagtttaagaaggaaggacgctctggacagggaaaaatcacgaaatctatcggagcttat
atcgataacgatggagagatcatcgtgccacttgtgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACGGTCCAACAGTGAAGAATGCCC 432 377 49 Antisense
CTAATGAATCTGCGTCTTCTCATCT 321 227 133 Antisense
CCTGCGGCTACGACTACTACGAGCC 598 263 188 Antisense
AAGTCGAAAATCGTGCTCGCCGTGT 665 157 220 Antisense
TGCTCGGTTTCCTACTTCATATGCA 640 579 244 Antisense
CGAGAAGGACTGCATCTACGAGGCC 339 251 300 Antisense
CTACGAGGCCACCGAAGTCGACGGA 651 225 315 Antisense
AATGGGCGTGGAGCTCGGAGATCAA 538 163 353 Antisense
GGCTCACGACGATAAGTACACGCTG 414 537 378 Antisense
TACACGCTGTCGGCCGAGTTTAAGA 355 639 394 Antisense
GAGAGATCATCGTGCCACTTGTGAA 399 395 482 Antisense
Sequence Source Affymetrix Proprietary Database