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[184228_at]

Description F53G12.9 /REP_DB=WormBase Gene ID /WP=CE11022 /TR=O01801 /GB=AAB54164.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 02      4grp_total > 10      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 41.8
(0)
A 		70.9
(3)
A 		12.3
(-3)
A 		69.1
(2.8)
A 		59
B 29.5
(-3)
A 		31.9
(-2.5)
A 		55.1
(2.2)
A 		58.8
(3)
A 		29
C 27.8
(-1.7)
A 		13.6
(-3)
A 		30.2
(-1.5)
A 		78.6
(3)
A 		65
avr 33
(-2.9) 		38.8
(-2) 		32.5
(-3) 		68.8
(3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 58
1 2 3 4 5 6 range
A 58.9
(0.8)
A 		70.2
(3)
A 		41.2
(-2.7)
A 		62.6
(1.5)
A 		42.3
(-2.5)
A 		39.9
(-3)
A 		30
B 42.6
(1.3)
A 		49.9
(2.7)
A 		27.3
(-1.8)
A 		42.3
(1.2)
A 		51.4
(3)
A 		21.1
(-3)
A 		30
C 52
(3)
A 		47.1
(2.3)
A 		12.3
(-3)
A 		14.2
(-2.7)
A 		22.1
(-1.5)
A 		46.6
(2.2)
A 		40
avr 51.2
(2.1) 		55.7
(3) 		26.9
(-3) 		39.7
(-0.3) 		38.6
(-0.6) 		35.9
(-1.1) 		29
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 15.2
(2.9)
A 		15.4
(3)
A 		3.2
(-3)
A 		10.1
(0.4)
A 		12
B 9.4
(-1.2)
A 		7.9
(-3)
A 		12.8
(3)
A 		12.5
(2.6)
A 		5
C 9.2
(0.4)
A 		3.9
(-3)
A 		10.5
(1.3)
A 		13.2
(3)
A 		9
avr 11.3
(1.8) 		9.1
(-2.4) 		8.8
(-3) 		11.9
(3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 10.9
(-2.3)
A 		25
(3)
A 		9
(-3)
A 		15.9
(-0.4)
A 		12.8
(-1.6)
A 		9.7
(-2.7)
A 		16
B 14.8
(0.4)
A 		16.6
(1.2)
A 		6.6
(-3)
A 		11.2
(-1.1)
A 		20.9
(3)
A 		7.9
(-2.5)
A 		14
C 19.3
(3)
A 		14.4
(1.2)
A 		3.1
(-2.9)
A 		2.9
(-3)
A 		7.1
(-1.5)
A 		15.6
(1.6)
A 		16
avr 15
(1.2) 		18.7
(3) 		6.2
(-3) 		10
(-1.2) 		13.6
(0.6) 		11.1
(-0.6) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184228_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2597
Sequence Type Exemplar sequence
Representative Public ID F53G12.9 NCBI
Target Description F53G12.9 /REP_DB=WormBase Gene ID /WP=CE11022 /TR=O01801 /GB=AAB54164.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058276(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058276 NCBI Caenorhabditis elegans F53G12.9 (F53G12.9) mRNA, complete cds. 11/11 None
SNAP00000020921 ENSEMBL cdna:SNAP chromosome:CEL140:I:112365:113661:1 11/11 None
GENEFINDER00000020935 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:112365:114681:1 11/11 None
F53G12.9 ENSEMBL cdna:known chromosome:CEL140:I:112365:113699:1 gene:F53G12.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:112364-113124(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F53G12.9
Entrez Gene 171603 Entrez gene
SwissProt O01801 EMBL-EBI
WormBase CE30376 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM15578 Hypothetical protein F53G12.9 [Caenorhabditis elegans] ref|NP_490677.2| F53G12.9 [Caenorhabditis elegans] 1.0E-136
blast AAM15578 Hypothetical protein F53G12.9 [Caenorhabditis elegans] ref|NP_490677.2| F53G12.9 [Caenorhabditis elegans] 1.0E-135
blast AAM15578 Hypothetical protein F53G12.9 [Caenorhabditis elegans] ref|NP_490677.2| F53G12.9 [Caenorhabditis elegans] 4.0E-95
blast T29033 hypothetical protein F53G12.9 - Caenorhabditis elegans 2.0E-74
blast T29033 hypothetical protein F53G12.9 - Caenorhabditis elegans 3.0E-74
blast T29033 hypothetical protein F53G12.9 - Caenorhabditis elegans 6.0E-74
blast T29032 hypothetical protein F53G12.8 - Caenorhabditis elegans 3.0E-68
blast T29032 hypothetical protein F53G12.8 - Caenorhabditis elegans 3.0E-40
Sequence
Target Sequence 
>C. ELEGANS:184228_AT
gaatcgtaaaaccgctttgctgactactctcccgagaacagtcactgttccctactatgc
tccatcctgtttatttctagaaacttttcttcggagcaagctaattccctatgaaacaac
gcaatgttctctctacaatgtactccctcgtgagcacctatacccattgattgatgtcga
tgggtatttcttcaaaaatttgatggaaggacttgattatctgctcgccaaatatggaaa
atctctggactcgggcctgactccaaaagagcgagcacaggctctcgcattgagcgcttt
gctcgacgagcttacttggatgctcgcctactcgcgaggccaagatttcacgtggctccg
cgatgatcgtaagattatcgaagacttcgggcttgttcagctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATCGTAAAACCGCTTTGCTGACT 341 329 15 Antisense
GACTACTCTCCCGAGAACAGTCACT 230 373 36 Antisense
TATGCTCCATCCTGTTTATTTCTAG 632 655 70 Antisense
CTCTACAATGTACTCCCTCGTGAGC 63 227 145 Antisense
GAGCACCTATACCCATTGATTGATG 624 387 166 Antisense
GAAGGACTTGATTATCTGCTCGCCA 452 333 220 Antisense
ATGGAAAATCTCTGGACTCGGGCCT 250 53 248 Antisense
GAGCGAGCACAGGCTCTCGCATTGA 156 387 283 Antisense
GCATTGAGCGCTTTGCTCGACGAGC 339 307 301 Antisense
AGGCCAAGATTTCACGTGGCTCCGC 23 53 351 Antisense
CGAAGACTTCGGGCTTGTTCAGCTC 319 251 393 Antisense
Sequence Source Affymetrix Proprietary Database