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[184226_at]

Description F53G12.3 /REP_DB=WormBase Gene ID /WP=CE24999 /TR=O01795 /GB=AAB54159.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 28      4grp_total > 39      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 24.7
(3)
A 		16.9
(-3)
A 		23.7
(2.2)
A 		18.1
(-2.1)
A 		8
B 88
(3)
A 		14.3
(-3)
A 		16.1
(-2.9)
A 		18.5
(-2.7)
A 		74
C 76.7
(2.1)
A 		43.1
(-2.5)
A 		39.1
(-3)
A 		83.2
(3)
A 		44
avr 63.1
(3) 		24.8
(-3) 		26.3
(-2.8) 		39.9
(-0.6) 		38

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 65
1 2 3 4 5 6 range
A 71.4
(3)
A 		35.1
(-0.7)
A 		38
(-0.4)
A 		30.7
(-1.2)
A 		14.4
(-2.8)
A 		12.7
(-3)
A 		59
B 25.7
(1.1)
A 		11.1
(-2)
A 		6.5
(-3)
A 		22.9
(0.5)
A 		34.8
(3)
A 		32.8
(2.6)
A 		28
C 44.3
(0.6)
A 		55.7
(3)
A 		42.5
(0.3)
A 		26.6
(-3)
A 		54.8
(2.8)
A 		44.6
(0.7)
A 		29
avr 47.1
(3) 		34
(-0.9) 		29
(-2.3) 		26.7
(-3) 		34.7
(-0.6) 		30
(-2) 		20
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 25
1 2 3 4 range
A 9
(3)
A 		3.7
(-2)
A 		6.2
(0.3)
A 		2.7
(-3)
A 		6
B 28.1
(3)
A 		3.5
(-3)
A 		3.7
(-3)
A 		3.9
(-2.9)
A 		25
C 25.4
(3)
A 		12.4
(-3)
A 		13.6
(-2.4)
A 		14
(-2.3)
A 		13
avr 20.8
(3) 		6.5
(-3) 		7.8
(-2.5) 		6.9
(-2.8) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 13.2
(3)
A 		12.5
(2.6)
A 		8.3
(0.1)
A 		7.8
(-0.2)
A 		4.3
(-2.3)
A 		3.1
(-3)
A 		10
B 8.9
(0.5)
A 		3.7
(-2)
A 		1.6
(-3)
A 		6.1
(-0.9)
A 		14.2
(3)
A 		12.2
(2)
A 		13
C 16.4
(2.4)
A 		17
(2.7)
A 		10.8
(-0.3)
A 		5.3
(-3)
A 		17.7
(3)
A 		14.9
(1.6)
A 		12
avr 12.8
(3) 		11.1
(1.4) 		6.9
(-2.5) 		6.4
(-3) 		12.1
(2.3) 		10.1
(0.5) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184226_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2593
Sequence Type Exemplar sequence
Representative Public ID F53G12.3 NCBI
Target Description F53G12.3 /REP_DB=WormBase Gene ID /WP=CE24999 /TR=O01795 /GB=AAB54159.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058283(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058283 NCBI Caenorhabditis elegans F53G12.3 (F53G12.3) mRNA, complete cds. 11/11 None
GENEFINDER00000020932 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:135566:144565:1 11/11 None
SNAP00000020928 ENSEMBL cdna:SNAP chromosome:CEL140:I:141716:144565:1 11/11 None
F53G12.3 ENSEMBL cdna:known chromosome:CEL140:I:137845:144565:1 gene:F53G12.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:137844-144565(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F53G12.3
Entrez Gene 186188 Entrez gene
SwissProt O01795 EMBL-EBI
WormBase CE24999 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4601 peroxidase activity inferred from electronic annotation QuickGO AmiGO
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK73871 Hypothetical protein F53G12.3 [Caenorhabditis elegans] ref|NP_490684.1| F53G12.3 [Caenorhabditis elegans] 0.0
blast AAK73882 Blistered cuticle protein 3 [Caenorhabditis elegans] ref|NP_490686.3| BLIstered cuticle family member (bli-3) [Caenorhabditis elegans] 0.0
blast AAF71303 dual oxidase [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.088
Pfam IPR002007 EMBL-EBI Animal haem peroxidase 1.0E-126
Pfam IPR002916 EMBL-EBI Ferric reductase-like transmembrane component 3.8E-68
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.088
Pfam IPR002007 EMBL-EBI Animal haem peroxidase 1.0E-126
Pfam IPR002916 EMBL-EBI Ferric reductase-like transmembrane component 3.8E-68
Pfam IPR002916 EMBL-EBI Ferric reductase-like transmembrane component 3.8E-68
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_490684.1 7 600-622,994-1016,1031-1053,1074-1096,1135-1157,1170-1192,1196-1215
F53G12.3 7 600-622,994-1016,1031-1053,1074-1096,1135-1157,1170-1192,1196-1215
GENEFINDER00000020932 7 864-886,1258-1280,1295-1317,1338-1360,1399-1421,1434-1456,1460-1479
SNAP00000020928 6 123-145,160-182,203-225,264-286,299-321,325-344
Sequence
Target Sequence 
>C. ELEGANS:184226_AT
tgaatacagggtcaccattcccattaattcatatgaaaggaccatatggtgatggtaatc
aggaatggatgaattatgaagttgcaataatggttggagcaggaatcggagtgactccat
atgcatccacgcttgttgatcttgtacaaaaaacatcaagtgactcgtttcatagagttc
gatgtcgtaaagtatatttcctatgggtgtgttcgagtcacaagaactttgaatggtttg
tggatatgctgaaaaatgttgaaaatcaagcaaagccgggaatcctggagacacacatat
tcgtcactcagatgttccataagtttgatttaagaactactatgctttacatttgcgaga
agcacttccgtgccaccaactcaggcatatcaatgttcactggcctacacgctaagaatc
att

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAATACAGGGTCACCATTCCCATT 675 575 3920 Antisense
GGTCACCATTCCCATTAATTCATAT 390 503 3929 Antisense
TGCATCCACGCTTGTTGATCTTGTA 364 579 4041 Antisense
GTGACTCGTTTCATAGAGTTCGATG 124 483 4079 Antisense
TTTCCTATGGGTGTGTTCGAGTCAC 272 669 4116 Antisense
GCAAAGCCGGGAATCCTGGAGACAC 652 317 4189 Antisense
GGAGACACACATATTCGTCACTCAG 494 519 4206 Antisense
ATTCGTCACTCAGATGTTCCATAAG 586 9 4218 Antisense
GCCACCAACTCAGGCATATCAATGT 139 281 4291 Antisense
ATATCAATGTTCACTGGCCTACACG 87 21 4306 Antisense
ACTGGCCTACACGCTAAGAATCATT 109 97 4318 Antisense
Sequence Source Affymetrix Proprietary Database