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[184225_at]

Description F53G12.4 /REP_DB=WormBase Gene ID /WP=CE11012 /TR=O01796 /GB=AAB54160.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 16      4grp_total > 54      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 93
1 2 3 4 range
A 99
(3)
A 		28.1
(-2.1)
A 		51.1
(-0.4)
A 		15.1
(-3)
A 		84
B 94.3
(2.3)
A 		101.6
(3)
A 		55.9
(-1.3)
A 		38.1
(-3)
A 		64
C 91
(3)
A 		64.9
(1.1)
A 		24.4
(-1.9)
A 		9
(-3)
A 		82
avr 94.8
(3) 		64.9
(0.6) 		43.8
(-1.1) 		20.7
(-3) 		74

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 87
1 2 3 4 5 6 range
A 71.4
(3)
A 		65
(2.2)
A 		51.3
(0.6)
A 		42.3
(-0.5)
A 		21.3
(-3)
A 		48.8
(0.3)
A 		50
B 108.6
(3)
A 		84.3
(1.1)
A 		32.2
(-3)
A 		37.7
(-2.6)
A 		48.8
(-1.7)
A 		48.7
(-1.7)
A 		76
C 75.1
(3)
A 		59.3
(0.2)
A 		41.7
(-3)
A 		73.2
(2.7)
A 		65.4
(1.3)
A 		49.3
(-1.6)
A 		33
avr 85
(3) 		69.5
(0.9) 		41.7
(-3) 		51.1
(-1.7) 		45.2
(-2.5) 		48.9
(-2) 		43
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 35
1 2 3 4 range
A 36
(3)
A 		6.1
(-2.3)
A 		13.3
(-1)
A 		2.2
(-3)
A 		34
B 30.1
(3)
A 		25.1
(1.6)
A 		13
(-1.7)
A 		8.1
(-3)
A 		22
C 30.1
(3)
A 		18.7
(0.6)
A 		8.5
(-1.5)
A 		1.5
(-3)
A 		29
avr 32.1
(3) 		16.6
(-0.3) 		11.6
(-1.4) 		3.9
(-3) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 31
1 2 3 4 5 6 range
A 13.2
(-0.6)
A 		23.1
(3)
A 		11.2
(-1.3)
A 		10.7
(-1.5)
A 		6.4
(-3)
A 		11.9
(-1)
A 		17
B 37.8
(3)
A 		28.1
(1.1)
A 		7.8
(-3)
A 		10
(-2.6)
A 		19.8
(-0.6)
A 		18.1
(-0.9)
A 		30
C 27.8
(3)
A 		18.1
(-0.4)
A 		10.6
(-3)
A 		14.7
(-1.6)
A 		21.1
(0.7)
A 		16.5
(-0.9)
A 		17
avr 26.3
(3) 		23.1
(1.8) 		9.9
(-3) 		11.8
(-2.3) 		15.8
(-0.8) 		15.5
(-1) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184225_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2589
Sequence Type Exemplar sequence
Representative Public ID F53G12.4 NCBI
Target Description F53G12.4 /REP_DB=WormBase Gene ID /WP=CE11012 /TR=O01796 /GB=AAB54160.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058282(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058282 NCBI Caenorhabditis elegans F53G12.4 (F53G12.4) mRNA, complete cds. 10/11 None
SNAP00000020926 ENSEMBL cdna:SNAP chromosome:CEL140:I:134354:137282:1 11/11 None
GENEFINDER00000020932 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:135566:144565:1 11/11 None
F53G12.4 ENSEMBL cdna:known chromosome:CEL140:I:135566:136810:1 gene:F53G12.4 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:135565-137282(+) 99.21 100.0
Public Domain and Genome References
Gene Title
Ensembl F53G12.4
Entrez Gene 186189 Entrez gene
SwissProt O01796 EMBL-EBI
WormBase CE36696 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK73871 Hypothetical protein F53G12.3 [Caenorhabditis elegans] ref|NP_490684.1| F53G12.3 [Caenorhabditis elegans] 0.0
blast AAK73882 Blistered cuticle protein 3 [Caenorhabditis elegans] ref|NP_490686.3| BLIstered cuticle family member (bli-3) [Caenorhabditis elegans] 0.0
blast AAF71303 dual oxidase [Caenorhabditis elegans] 0.0
blast AAB54160 Hypothetical protein F53G12.4 [Caenorhabditis elegans] ref|NP_490683.2| F53G12.4 [Caenorhabditis elegans] 1.0E-136
blast AAB54160 Hypothetical protein F53G12.4 [Caenorhabditis elegans] ref|NP_490683.2| F53G12.4 [Caenorhabditis elegans] 1.0E-116
blast CAE61282 Hypothetical protein CBG05101 [Caenorhabditis briggsae] 3.0E-28
blast CAE61282 Hypothetical protein CBG05101 [Caenorhabditis briggsae] 5.0E-13
Protein Domains
Database ID Description E-Value
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.088
Pfam IPR002007 EMBL-EBI Animal haem peroxidase 1.0E-126
Pfam IPR002916 EMBL-EBI Ferric reductase-like transmembrane component 3.8E-68
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000020932 7 864-886,1258-1280,1295-1317,1338-1360,1399-1421,1434-1456,1460-1479
Sequence
Target Sequence 
>C. ELEGANS:184225_AT
tggagagggaacgactcgcacgtctatccaaccaggcagaagacgattccgacgattcat
cgtcctcctccgactcttccacgtcatccgcgtcgtcatcttccgaggagaagttgactt
tttcggatatcttggggaaacccacagcagctggcgcggagaaaatgatcgaactaagcc
aaattcccaacgcatggggtccgtcaaaatccgcgttagtcactgatgacgtggcaagca
cttctgacccacttccggcgattttcaacgcacatcttccgccgctctccatgggacgac
caccgctgacaaaaagcgcgaaaagtcgggaaatcgacgccattttcggctcggataacg
tggattatgatttagaggatttcgagcaggatggcgtggcgaagaaaaagtctatactgc
ctatggagaaaaagaaggaggaagcgaaaccaaaggaacaggagctagtggagccgttgg
tcttgtcagaaggagaatcaatcgacgagcttgaagatttcgacaccggtctacta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGAGAGGGAACGACTCGCACGTCT 405 547 332 Antisense
CACGTCTATCCAACCAGGCAGAAGA 479 199 350 Antisense
GCGTCGTCATCTTCCGAGGAGAAGT 105 293 421 Antisense
AACTAAGCCAAATTCCCAACGCATG 348 109 503 Antisense
CCGTCAAAATCCGCGTTAGTCACTG 78 255 532 Antisense
GATGACGTGGCAAGCACTTCTGACC 18 413 556 Antisense
CCACTTCCGGCGATTTTCAACGCAC 202 265 580 Antisense
TCGACGCCATTTTCGGCTCGGATAA 111 603 665 Antisense
GGATTTCGAGCAGGATGGCGTGGCG 343 509 708 Antisense
TAGTGGAGCCGTTGGTCTTGTCAGA 372 653 797 Antisense
TGAAGATTTCGACACCGGTCTACTA 311 569 843 Antisense
Sequence Source Affymetrix Proprietary Database