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[184222_s_at]

Description W03F11.3 /REP_DB=WormBase Gene ID /WP=CE28502 /TR=O01778 /GB=AAK18986.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 98      4grp_total > 35      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 64
1 2 3 4 range
A 28.5
(-2.9)
A 		59.6
(2.2)
A 		27.6
(-3)
A 		64.3
(3)
A 		37
B 47.3
(-1.2)
A 		92
(3)
A 		59.3
(-0.1)
A 		27.8
(-3)
A 		64
C 76
(3)
A 		64.2
(0.2)
A 		50.9
(-3)
A 		74.9
(2.7)
A 		25
avr 50.6
(-1.9) 		71.9
(3) 		45.9
(-3) 		55.7
(-0.7) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 180
1 2 3 4 5 6 range
A 11.8
(-3)
A 		32.7
(-2.3)
A 		60.8
(-1.4)
A 		71.7
(-1)
A 		149.5
(1.6)
P 		191.5
(3)
P 		180
B 21.8
(-3)
A 		33.8
(-2.3)
A 		21.4
(-3)
A 		55.5
(-1.1)
A 		121.5
(2.5)
P 		131.3
(3)
P 		110
C 40.9
(-2.2)
A 		26.7
(-3)
A 		85.9
(0.3)
A 		32.8
(-2.7)
A 		124.7
(2.5)
P 		133.3
(3)
P 		107
avr 24.8
(-3) 		31.1
(-2.7) 		56
(-1.5) 		53.3
(-1.7) 		131.9
(2.1) 		152
(3) 		127
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 10.4
(0.4)
A 		12.9
(3)
A 		7.2
(-3)
A 		9.4
(-0.7)
A 		6
B 15.1
(0.3)
A 		22.7
(3)
A 		13.7
(-0.2)
A 		5.9
(-3)
A 		17
C 25.2
(3)
A 		18.4
(-0.2)
A 		17.8
(-0.5)
A 		12.6
(-3)
A 		13
avr 16.9
(2.2) 		18
(3) 		12.9
(-0.5) 		9.3
(-3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 47
1 2 3 4 5 6 range
A 2.2
(-3)
A 		11.6
(-1.7)
A 		13.2
(-1.5)
A 		18.2
(-0.8)
A 		45.1
(2.8)
P 		46.6
(3)
P 		44
B 7.6
(-2.7)
A 		11.3
(-2.2)
A 		5.2
(-3)
A 		14.7
(-1.7)
A 		49.4
(3)
P 		49
(2.9)
P 		44
C 15.1
(-1.7)
A 		8.2
(-2.7)
A 		21.9
(-0.6)
A 		6.6
(-3)
A 		40.3
(2.3)
P 		44.6
(3)
P 		38
avr 8.3
(-3) 		10.4
(-2.7) 		13.4
(-2.2) 		13.2
(-2.2) 		44.9
(2.7) 		46.7
(3) 		38
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184222_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.125
Sequence Type Exemplar sequence
Representative Public ID W03F11.3 NCBI
Target Description W03F11.3 /REP_DB=WormBase Gene ID /WP=CE28502 /TR=O01778 /GB=AAK18986.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058543(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058543 NCBI Caenorhabditis elegans W03F11.3 (W03F11.3) mRNA, complete cds. 11/11 A 
SNAP00000024823 ENSEMBL cdna:SNAP chromosome:CEL140:I:2215542:2234847:-1 11/11 None
GENEFINDER00000024825 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:2223354:2234847:-1 11/11 None
W03F11.3 ENSEMBL cdna:known chromosome:CEL140:I:2233779:2234907:-1 gene:W03F11.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2233778-2234738(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl W03F11.3
Entrez Gene 171779 Entrez gene
SwissProt O01778 EMBL-EBI
WormBase CE28502 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE60309 Hypothetical protein CBG03898 [Caenorhabditis briggsae] 0.0
blast CAE60828 Hypothetical protein CBG04537 [Caenorhabditis briggsae] 0.0
blast CAB07591 Hypothetical protein F21H7.9 [Caenorhabditis elegans] ref|NP_507101.1| Guanylyl CYclase family member (gcy-20) [Caenorhabditis elegans] 0.0
blast AAK18986 Hypothetical protein W03F11.3 [Caenorhabditis elegans] ref|NP_490944.1| W03F11.3 [Caenorhabditis elegans] 1.0E-102
blast CAE60307 Hypothetical protein CBG03896 [Caenorhabditis briggsae] 2.0E-61
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 0.018
Pfam IPR000719 EMBL-EBI Protein kinase 1.0E-13
Pfam IPR001054 EMBL-EBI Guanylate cyclase 2.1E-86
Pfam IPR002557 EMBL-EBI Chitin binding Peritrophin-A 1.0E-13
Pfam IPR001828 EMBL-EBI Extracellular ligand-binding receptor 6.8E-32
Pfam IPR000719 EMBL-EBI Protein kinase 0.29
Pfam IPR000719 EMBL-EBI Protein kinase 1.0E-13
Pfam IPR001054 EMBL-EBI Guanylate cyclase 2.8E-73
Pfam IPR001828 EMBL-EBI Extracellular ligand-binding receptor 4.4E-28
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000024823 2 602-624,760-782
GENEFINDER00000024825 2 585-607,769-791
Sequence
Target Sequence 
>C. ELEGANS:184222_S_AT
gcaaatgaaatgcacgtcccctgatcaggatcttgtcgtttatcccagatggctgttctt
ctactcgcaaaacgaataccgtactccactttacgtccgattctttgttgcaaatcgtga
tccgcatactgttgcatacaaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAAATGAAATGCACGTCCCCTGAT 89 319 15 Antisense
CACGTCCCCTGATCAGGATCTTGTC 627 199 27 Antisense
GGATCTTGTCGTTTATCCCAGATGG 27 513 42 Antisense
TTATCCCAGATGGCTGTTCTTCTAC 215 681 54 Antisense
GCTGTTCTTCTACTCGCAAAACGAA 451 295 66 Antisense
GCAAAACGAATACCGTACTCCACTT 623 315 81 Antisense
TCCACTTTACGTCCGATTCTTTGTT 78 589 99 Antisense
TTACGTCCGATTCTTTGTTGCAAAT 380 667 105 Antisense
GTTGCAAATCGTGATCCGCATACTG 479 439 121 Antisense
TCGTGATCCGCATACTGTTGCATAC 564 605 129 Antisense
GATCCGCATACTGTTGCATACAACA 315 417 133 Antisense
Sequence Source Affymetrix Proprietary Database