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[184212_s_at]

Description C56C10.3 /REP_DB=WormBase Gene ID /WP=CE02568 /TR=Q18886 /GB=AAA68771.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 72      6grp_high1_10_10 > 28      4grp_highest_8_8 > 39      4grp_high_8_8 > 12      6grp_highest_nonorm > 40      6grp_total > 27      4grp_total > 04      6grp_highest_sub > Cu      6grp_cgc > 05      6grp_high1_sub > De      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 943
1 2 3 4 range
A 1033.8
(-1)
P 		786.5
(-2.6)
P 		724.2
(-3)
P 		1667
(3)
P 		943
B 994.2
(-1.3)
P 		829.9
(-3)
P 		826.8
(-3)
P 		1423.8
(3)
P 		597
C 1086.6
(-0.5)
P 		922.2
(-1.8)
P 		774.2
(-3)
P 		1512.5
(3)
P 		738
avr 1038.2
(-0.9) 		846.2
(-2.4) 		775.1
(-3) 		1534.4
(3) 		759

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 579
1 2 3 4 5 6 range
A 1027.1
(3)
P 		764.6
(0.3)
P 		575.6
(-1.7)
P 		448.4
(-3)
P 		503.5
(-2.4)
P 		610
(-1.3)
P 		579
B 986.9
(3)
P 		729.1
(0.1)
P 		631.7
(-1)
P 		454.5
(-3)
P 		663.5
(-0.6)
P 		662.5
(-0.7)
P 		532
C 1001.7
(3)
P 		723.3
(-0)
P 		531.5
(-2.1)
P 		449.1
(-3)
P 		537.7
(-2)
P 		698.8
(-0.3)
P 		553
avr 1005.2
(3) 		739
(0.1) 		579.6
(-1.6) 		450.7
(-3) 		568.2
(-1.7) 		657.1
(-0.8) 		555
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 206
1 2 3 4 range
A 376.4
(3)
P 		170.7
(-3)
P 		189.3
(-2.5)
P 		244
(-0.9)
P 		206
B 317.1
(3)
P 		205
(-2.4)
P 		191.6
(-3)
P 		303.4
(2.3)
P 		126
C 359.7
(3)
P 		265.1
(-2.4)
P 		269.8
(-2.2)
P 		255
(-3)
P 		105
avr 351.1
(3) 		213.6
(-3) 		216.9
(-2.9) 		267.5
(-0.6) 		138

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 281
1 2 3 4 5 6 range
A 189.3
(-0.1)
P 		271.9
(3)
P 		125.3
(-2.6)
P 		113.7
(-3)
P 		151.9
(-1.6)
P 		148.5
(-1.7)
P 		158
B 343.4
(3)
P 		242.7
(0.3)
P 		153.4
(-2.1)
P 		120.4
(-3)
P 		269.6
(1)
P 		247
(0.4)
P 		223
C 371.3
(3)
P 		220.7
(-0.2)
P 		135.3
(-2)
P 		90.3
(-3)
P 		174
(-1.2)
P 		233.9
(0.1)
P 		281
avr 301.3
(3) 		245.1
(1.3) 		138
(-2.1) 		108.1
(-3) 		198.5
(-0.2) 		209.8
(0.2) 		193
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184212_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4747
Sequence Type Exemplar sequence
Representative Public ID C56C10.3 NCBI
Target Description C56C10.3 /REP_DB=WormBase Gene ID /WP=CE02568 /TR=Q18886 /GB=AAA68771.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062936(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062936 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-309) (tag-309) mRNA, complete cds. 11/11 None
SNAP00000022065 ENSEMBL cdna:SNAP chromosome:CEL140:II:6589656:6590707:1 11/11 None
GENEFINDER00000022080 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6589656:6590707:1 11/11 None
C56C10.3.1 ENSEMBL cdna:known chromosome:CEL140:II:6589235:6591029:1 gene:C56C10.3 11/11 None
C56C10.3.2 ENSEMBL cdna:known chromosome:CEL140:II:6589653:6591016:1 gene:C56C10.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6589652-6590704(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol tag-309
Ensembl C56C10.3
Entrez Gene 174091 Entrez gene
SwissProt Q18886 EMBL-EBI
WormBase CE02568 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:266698_AT SNF7 family protein at
CANINE:1589398_AT similar to Protein c20orf178 cfa
CANINE_2:CFA.4035.1.A1_AT similar to Protein c20orf178 cfa
CANINE_2:CFAAFFX.12085.1.S1_AT similar to Protein c20orf178 cfa
DROSOPHILA_2:1638126_AT dm
DROSGENOME1:152547_AT dm
CHICKEN:GGAAFFX.13192.1.S1_AT chromatin modifying protein 4B gga
HG-U133_PLUS_2:225119_AT chromatin modifying protein 4B hs
HG-U133B:225119_AT chromatin modifying protein 4B hs
HU35KSUBA:RC_AA464245_AT chromatin modifying protein 4B hs
U133_X3P:HS.178137.2.S1_3P_AT chromatin modifying protein 4B hs
U133_X3P:HS.164478.2.S1_3P_AT chromatin modifying protein 4B hs
HG-U133_PLUS_2:225498_AT chromatin modifying protein 4B hs
HG-U133B:225498_AT chromatin modifying protein 4B hs
HG-U95B:45298_AT chromatin modifying protein 4B hs
HG-U95E:77390_AT chromatin modifying protein 4B hs
HG-U95E:80658_AT chromatin modifying protein 4B hs
MU11KSUBA:AA178189_S_AT chromatin modifying protein 4B mm
MU11KSUBB:MSA.10058.0_S_AT chromatin modifying protein 4B mm
MG-U74AV2:103555_AT chromatin modifying protein 4B mm
MOE430A:1451988_S_AT chromatin modifying protein 4B mm
MOUSE430A_2:1451988_S_AT chromatin modifying protein 4B mm
MOUSE430_2:1451988_S_AT chromatin modifying protein 4B mm
MOE430A:1426240_AT chromatin modifying protein 4B mm
MOUSE430A_2:1426240_AT chromatin modifying protein 4B mm
MOUSE430_2:1426240_AT chromatin modifying protein 4B mm
MG-U74AV2:99472_AT Chromatin modifying protein 4B, mRNA (cDNA clone MGC:19416 IMAGE:3485793) mm
MG-U74AV2:99473_AT Chromatin modifying protein 4B, mRNA (cDNA clone MGC:19416 IMAGE:3485793) mm
MU19KSUBA:TC25197_I_AT Chromatin modifying protein 4B, mRNA (cDNA clone MGC:19416 IMAGE:3485793) mm
MU19KSUBC:TC37725_AT Chromatin modifying protein 4B, mRNA (cDNA clone MGC:19416 IMAGE:3485793) mm
MU19KSUBC:TC37725_G_AT Chromatin modifying protein 4B, mRNA (cDNA clone MGC:19416 IMAGE:3485793) mm
MU19KSUBC:TC37726_AT Chromatin modifying protein 4B, mRNA (cDNA clone MGC:19416 IMAGE:3485793) mm
RAE230A:1388838_AT Similar to 2010012F05Rik protein rn
RAT230_2:1388838_AT Similar to 2010012F05Rik protein rn
RG-U34B:RC_AA943752_AT Similar to 2010012F05Rik protein rn
YEAST_2:1779219_AT One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Sc
Protein Similarities
Method ID Description E-Value
blast AAA68771 Hypothetical protein C56C10.3 [Caenorhabditis elegans] ref|NP_495337.1| Temporarily Assigned Gene name family member (tag-309) [Caenorhabditis elegans] 1.0E-108
blast CAE60666 Hypothetical protein CBG04319 [Caenorhabditis briggsae] 1.0E-98
Protein Domains
Database ID Description E-Value
Pfam IPR005024 EMBL-EBI Snf7 1.9E-72
Sequence
Target Sequence 
>C. ELEGANS:184212_S_AT
cgaaaaacaagcgaatggctctccagtgtttgaatagaaagagaaatttcgagaagcagt
tggcccatattgacggagttttgtctactatcggatatcagagagaagccctcgaaaatg
cttcaacgaatgctgaagttctcaatgttatgggaactgctagcaaggcgttgaaagcgg
ctcataacaacatggatatcgaccaagttcatgatttgatggaagacatagctgagcaac
aagaagtggcaaacgaaatcgctgaagctatttcaaaccctgtcggcttcagcaccgcag
ttgacgatgacgatttgatgcgcgagttggaggctcttgaacaggaagaacttgacaaag
aattgcttgatgcgagagctccaccagtcacgcttccggatactccaaatattgcacttc
cagcagttccggcttccagaccgagagcgaaaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGAAAAACAAGCGAATGGCTCTCCA 523 251 179 Antisense
GACGGAGTTTTGTCTACTATCGGAT 200 375 250 Antisense
ATGGGAACTGCTAGCAAGGCGTTGA 404 49 328 Antisense
GCAAGGCGTTGAAAGCGGCTCATAA 327 321 341 Antisense
GGCTCATAACAACATGGATATCGAC 188 539 357 Antisense
GGATATCGACCAAGTTCATGATTTG 198 511 372 Antisense
AATCGCTGAAGCTATTTCAAACCCT 192 169 435 Antisense
TCAGCACCGCAGTTGACGATGACGA 330 625 467 Antisense
GACGATGACGATTTGATGCGCGAGT 346 377 481 Antisense
GATGCGCGAGTTGGAGGCTCTTGAA 192 415 495 Antisense
CGGCTTCCAGACCGAGAGCGAAAGA 351 241 608 Antisense
Sequence Source Affymetrix Proprietary Database