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[184186_at]

Description F25F1.2 /REP_DB=WormBase Gene ID /WP=CE17701 /TR=O61714 /GB=AAC16425.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 4grp_high_8_8 > 20      6grp_total > 35      4grp_total > 28      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 89
1 2 3 4 range
A 135.1
(0.3)
P 		154.1
(1.7)
M 		90.7
(-3)
P 		170.9
(3)
P 		80
B 115.9
(2.7)
P 		118
(3)
P 		105.8
(1)
A 		81.9
(-3)
A 		36
C 118.3
(1.2)
P 		88.5
(-3)
A 		122.9
(1.9)
M 		130.8
(3)
A 		42
avr 123.1
(1.7) 		120.2
(0.8) 		106.5
(-3) 		127.9
(3) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 134
1 2 3 4 5 6 range
A 101.3
(0.5)
P 		157.5
(3)
P 		64.7
(-1.2)
A 		124.6
(1.5)
A 		23.5
(-3)
A 		116.8
(1.2)
A 		134
B 85.8
(2)
A 		93.6
(3)
A 		48.4
(-3)
A 		66.2
(-0.6)
A 		48.1
(-3)
A 		68.6
(-0.3)
A 		46
C 119.4
(1.6)
A 		102.7
(0.4)
A 		140
(3)
P 		54.1
(-3)
A 		83.1
(-1)
P 		56.9
(-2.8)
A 		86
avr 102.2
(1.6) 		117.9
(3) 		84.4
(-0) 		81.6
(-0.3) 		51.6
(-3) 		80.8
(-0.4) 		66
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 32
1 2 3 4 range
A 49.2
(3)
P 		33.4
(-0.7)
M 		23.7
(-3)
P 		25
(-2.7)
P 		26
B 37
(3)
P 		29.2
(0.6)
P 		24.5
(-0.8)
A 		17.5
(-3)
A 		20
C 39.2
(2)
P 		25.4
(-2)
A 		42.8
(3)
M 		22
(-3)
A 		21
avr 41.8
(3) 		29.3
(-0.7) 		30.3
(-0.4) 		21.5
(-3) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 18.7
(-1.6)
P 		56
(3)
P 		14.1
(-2.1)
A 		31.6
(0)
A 		7.1
(-3)
A 		28.4
(-0.4)
A 		49
B 29.9
(2.6)
A 		31.2
(3)
A 		11.8
(-3)
A 		17.5
(-1.2)
A 		19.5
(-0.6)
A 		25.6
(1.3)
A 		19
C 44.3
(3)
A 		31.3
(0.7)
A 		35.6
(1.4)
P 		10.9
(-3)
A 		26.9
(-0.1)
P 		19
(-1.5)
A 		33
avr 31
(0.6) 		39.5
(3) 		20.5
(-2.3) 		20
(-2.4) 		17.8
(-3) 		24.3
(-1.2) 		22
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184186_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.316
Sequence Type Exemplar sequence
Representative Public ID F25F1.2 NCBI
Target Description F25F1.2 /REP_DB=WormBase Gene ID /WP=CE17701 /TR=O61714 /GB=AAC16425.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026367(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026367 NCBI Caenorhabditis elegans K03E5.5a (K03E5.5) mRNA, complete cds. 11/11 None
K03E5.5a ENSEMBL cdna:known chromosome:CEL140:I:3375100:3402865:-1 gene:K03E5.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
184929 2 1 NM_001026367 182445_at (11), 184186_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3394660-3399254(-) 97.1 100.0
Public Domain and Genome References
Ensembl K03E5.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001021538 K03E5.5a [Caenorhabditis elegans] 0.0
blast NP_001021539 K03E5.5b [Caenorhabditis elegans] 0.0
blast T33824 hypothetical protein W05F2.4 - Caenorhabditis elegans 0.0
Sequence
Target Sequence 
>C. ELEGANS:184186_AT
aaagagtctgctccagcatcaactccagcttcagcctcagcgccaactccagctccagcc
acatccaaaccaactccgactgcagtgaaaccagctgaaactccaaagacgcctgcaaaa
ccagttcctgaaaaagtccaggttcctgctgttgtcaaagttgctccagaaaaagtgaaa
tcacctactactgctgttgagccaaagaaatcagctccagctgtacctactgtgactgaa
aaaccaaaagaagctccgaaggagactccaaagccaggaatcgaaaagaaaaaagtgcta
ctgaaaaagaagaaaatcattccgattgatgatgaagttgaggcaccaaaagtgctcagc
gagcagaacactgaaatatcaaaagtattttcaagaagagcatggggagcacaaaaaacc
gagaaaaagatgaaattgagaaatgagatgaagggaatttcaattcgttgcaaggcggct
ggaaatcaaacaactcaaattgaagagcactatcgtaggaaaccacaagccgaaagagct
acggttcttgtggatactcct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAGAGTCTGCTCCAGCATCAACTC 323 123 1348 Antisense
CAAACCAACTCCGACTGCAGTGAAA 188 193 1413 Antisense
ACCAGCTGAAACTCCAAAGACGCCT 695 85 1437 Antisense
AAAGACGCCTGCAAAACCAGTTCCT 346 123 1452 Antisense
AAGTCCAGGTTCCTGCTGTTGTCAA 503 157 1481 Antisense
GAAATCACCTACTACTGCTGTTGAG 416 363 1524 Antisense
CTCCAGCTGTACCTACTGTGACTGA 522 235 1562 Antisense
TTCAATTCGTTGCAAGGCGGCTGGA 509 601 1806 Antisense
GGCGGCTGGAAATCAAACAACTCAA 196 541 1821 Antisense
GCACTATCGTAGGAAACCACAAGCC 8 315 1854 Antisense
AGCTACGGTTCTTGTGGATACTCCT 621 79 1884 Antisense
Sequence Source Affymetrix Proprietary Database