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[184168_at]

Description R02F2.8 /REP_DB=WormBase Gene ID /WP=CE00772 /TR=Q21652 /GB=AAA50724.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 87      6grp_high1_10_10 > 78      4grp_highest_8_8 > 23      4grp_high_8_8 > 05      6grp_highest_nonorm > 94      6grp_total > 79      4grp_total > 03      6grp_highest_sub > Da      6grp_high1_sub > Cu      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2743
1 2 3 4 range
A 574.3
(-2.5)
P 		405.5
(-2.8)
P 		335.4
(-3)
P 		3041.4
(3)
P 		2706
B 603.4
(-2.5)
P 		428.3
(-3)
P 		458.8
(-2.9)
P 		2598.1
(3)
P 		2170
C 670.5
(-1.9)
P 		298.2
(-3)
P 		384.6
(-2.7)
P 		2344.4
(3)
P 		2046
avr 616.1
(-2.4) 		377.3
(-3) 		392.9
(-3) 		2661.3
(3) 		2284

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 973
1 2 3 4 5 6 range
A 502.1
(-1.6)
P 		432.4
(-2)
P 		285.3
(-3)
P 		306.6
(-2.9)
P 		620.6
(-0.8)
P 		1198.1
(3)
P 		913
B 437.7
(-1.5)
P 		347.6
(-2.1)
P 		225.3
(-3)
P 		369.2
(-2)
P 		672.2
(0.3)
P 		1048.4
(3)
P 		823
C 398.5
(-2.2)
P 		341.6
(-2.7)
P 		297.2
(-3)
P 		300.7
(-3)
P 		593.1
(-0.7)
P 		1061.3
(3)
P 		764
avr 446.1
(-1.7) 		373.9
(-2.2) 		269.3
(-3) 		325.5
(-2.6) 		628.6
(-0.4) 		1102.6
(3) 		833
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 468
1 2 3 4 range
A 209.1
(-1)
P 		88
(-3)
P 		87.7
(-3)
P 		445.2
(3)
P 		358
B 192.4
(-1.8)
P 		105.8
(-3)
P 		106.3
(-3)
P 		553.6
(3)
P 		448
C 222
(-0.4)
P 		85.7
(-3)
P 		134.1
(-2.1)
P 		395.3
(3)
P 		310
avr 207.8
(-1.1) 		93.2
(-3) 		109.4
(-2.7) 		464.7
(3) 		372

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 336
1 2 3 4 5 6 range
A 92.6
(-2.2)
P 		153.8
(-0.6)
P 		62.1
(-3)
P 		77.8
(-2.6)
P 		187.3
(0.3)
P 		291.7
(3)
P 		230
B 152.3
(-1.3)
P 		115.7
(-1.9)
P 		54.7
(-3)
P 		97.8
(-2.2)
P 		273.1
(0.9)
P 		391
(3)
P 		336
C 147.7
(-1.2)
P 		104.2
(-2.1)
P 		75.6
(-2.7)
P 		60.5
(-3)
P 		191.9
(-0.3)
P 		355.3
(3)
P 		295
avr 130.9
(-1.6) 		124.6
(-1.7) 		64.1
(-3) 		78.7
(-2.7) 		217.4
(0.3) 		346
(3) 		282
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184168_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6483
Sequence Type Exemplar sequence
Representative Public ID R02F2.8 NCBI
Target Description R02F2.8 /REP_DB=WormBase Gene ID /WP=CE00772 /TR=Q21652 /GB=AAA50724.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065772(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065772 NCBI Caenorhabditis elegans R02F2.8 (R02F2.8) mRNA, complete cds. 11/11 None
SNAP00000033071 ENSEMBL cdna:SNAP chromosome:CEL140:III:5492950:5495209:-1 11/11 None
GENEFINDER00000033086 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5492950:5495209:-1 11/11 None
R02F2.8 ENSEMBL cdna:known chromosome:CEL140:III:5492906:5495224:-1 gene:R02F2.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5492948-5495208(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R02F2.8
Entrez Gene 187530 Entrez gene
SwissProt Q21652 EMBL-EBI
WormBase CE00772 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1633189_AT dm
DROSGENOME1:150541_AT dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6865 amino acid transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5279 amino acid-polyamine transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA50724 Hypothetical protein R02F2.8 [Caenorhabditis elegans] ref|NP_498173.1| R02F2.8 [Caenorhabditis elegans] 0.0
blast CAE61373 Hypothetical protein CBG05215 [Caenorhabditis briggsae] emb|CAE61365.1| Hypothetical protein CBG05207 [Caenorhabditis briggsae] 0.0
blast CAE61366 Hypothetical protein CBG05208 [Caenorhabditis briggsae] 1.0E-109
Protein Domains
Database ID Description E-Value
Pfam IPR002422 EMBL-EBI Amino acid/polyamine transporter II 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498173.1 11 23-45,55-77,111-133,148-170,177-196,216-233,246-268,288-310,331-353,357-379,414-436
R02F2.8 11 23-45,55-77,111-133,148-170,177-196,216-233,246-268,288-310,331-353,357-379,414-436
SNAP00000033071 11 23-45,55-77,111-133,148-170,177-196,216-233,246-268,288-310,331-353,357-379,414-436
GENEFINDER00000033086 11 23-45,55-77,111-133,148-170,177-196,216-233,246-268,288-310,331-353,357-379,414-436
Sequence
Target Sequence 
>C. ELEGANS:184168_AT
gattgctgttcacgtgatcactaccatcgtcatagtgatgtctccaccgattcagcaggt
tgagcagcttctcaaggttccgcataaattcggagtcaagagattcttggttcgatccat
tcttttctggtttgtcattttcattggattatctattccccactttggaccggttttgga
cttgatcggagcatccaccatggttctaatgaccttaattttgccaccaattttctatct
ctcaattcgcactcaggaaataatctggctgcagggtaatgaaaagtctgatgctgcaga
gccgactcacgagagagcaacgtttaaggaaattctacgtctgactccaaaactgatcct
tgccctaaacgcagctgtgctgatatttggaatcatcggtggtacgctgtctactgtaac
atctgtcattcggcttgtagggtctgatatggcagctccttgttattatcaatactttac
taaaggtctaccattctctggcgacaacagtggatctgttagctgctgtggtacattccg
aa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTGCTGTTCACGTGATCACTACC 235 433 900 Antisense
GTCATAGTGATGTCTCCACCGATTC 396 467 928 Antisense
GTTCGATCCATTCTTTTCTGGTTTG 240 441 1009 Antisense
ATTGGATTATCTATTCCCCACTTTG 122 5 1042 Antisense
TCGGAGCATCCACCATGGTTCTAAT 79 603 1085 Antisense
TTGCCACCAATTTTCTATCTCTCAA 125 703 1120 Antisense
TTGCCCTAAACGCAGCTGTGCTGAT 617 697 1259 Antisense
GAATCATCGGTGGTACGCTGTCTAC 4 329 1289 Antisense
ATCTGTCATTCGGCTTGTAGGGTCT 14 35 1320 Antisense
AGGGTCTGATATGGCAGCTCCTTGT 649 57 1338 Antisense
GTTAGCTGCTGTGGTACATTCCGAA 410 447 1417 Antisense
Sequence Source Affymetrix Proprietary Database