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[184167_s_at]

Description W04B5.4 /REP_DB=WormBase Gene ID /WP=CE20141 /TR=Q9UA63 /GB=AAC68918.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 12      6grp_high1_10_10 > 20      4grp_highest_8_8 > 19      4grp_high_8_8 > 63      6grp_highest_nonorm > 61      6grp_total > 50      4grp_total > 56      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 264
1 2 3 4 range
A 502
(3)
P 		494.4
(2.2)
P 		462
(-1.4)
P 		447.8
(-3)
P 		54
B 427.8
(-2.7)
P 		416.5
(-3)
P 		680.8
(3)
P 		448.6
(-2.3)
P 		264
C 507.3
(-0.7)
P 		485.9
(-3)
P 		542
(3)
P 		487.4
(-2.8)
P 		56
avr 479
(-1.9) 		465.6
(-2.7) 		561.6
(3) 		461.3
(-3) 		100

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 322
1 2 3 4 5 6 range
A 569.8
(2.9)
P 		451.2
(0.5)
P 		574
(3)
P 		418.3
(-0.1)
P 		274.8
(-3)
P 		384.4
(-0.8)
P 		299
B 477.5
(0.5)
P 		429.8
(-0.7)
P 		584.7
(3)
P 		436.3
(-0.5)
P 		330.4
(-3)
P 		427.5
(-0.7)
P 		254
C 477.2
(1)
P 		438.9
(0.3)
P 		467.1
(0.8)
P 		585.2
(3)
P 		262.9
(-3)
P 		505.1
(1.5)
P 		322
avr 508.2
(2.2) 		440
(0.6) 		541.9
(3) 		479.9
(1.5) 		289.4
(-3) 		439
(0.6) 		253
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 123
1 2 3 4 range
A 182.8
(3)
P 		107.3
(-0.9)
P 		120.8
(-0.2)
P 		65.6
(-3)
P 		117
B 136.4
(0.9)
P 		102.9
(-2.3)
P 		157.8
(3)
P 		95.6
(-3)
P 		62
C 167.9
(1.8)
P 		139.7
(0.2)
P 		188.9
(3)
P 		82.2
(-3)
P 		107
avr 162.4
(3) 		116.6
(-0.4) 		155.8
(2.5) 		81.1
(-3) 		81

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 94
1 2 3 4 5 6 range
A 105
(-1.3)
P 		160.5
(3)
P 		125
(0.3)
P 		106.1
(-1.2)
P 		82.9
(-3)
P 		93.6
(-2.2)
P 		78
B 166.2
(3)
P 		143.1
(0.3)
P 		142
(0.1)
P 		115.6
(-3)
P 		134.2
(-0.8)
P 		159.4
(2.2)
P 		51
C 176.9
(3)
P 		133.9
(0.2)
P 		118.9
(-0.8)
P 		117.7
(-0.9)
P 		85.1
(-3)
P 		169.1
(2.5)
P 		92
avr 149.4
(3) 		145.8
(2.6) 		128.6
(0.4) 		113.1
(-1.5) 		100.7
(-3) 		140.7
(1.9) 		49
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 184167_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6282
Sequence Type Exemplar sequence
Representative Public ID W04B5.4 NCBI
Target Description W04B5.4 /REP_DB=WormBase Gene ID /WP=CE20141 /TR=Q9UA63 /GB=AAC68918.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065149(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065149 NCBI Caenorhabditis elegans W04B5.4 (W04B5.4) mRNA, complete cds. 11/11 None
GENEFINDER00000019384 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2406439:2410951:-1 11/11 None
SNAP00000019368 ENSEMBL cdna:SNAP chromosome:CEL140:III:2406443:2410951:-1 9/11 None
W04B5.4 ENSEMBL cdna:known chromosome:CEL140:III:2406320:2410963:-1 gene:W04B5.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2406438-2409812(-) 96.8 96.8
Public Domain and Genome References
Gene Title
Ensembl W04B5.4
Entrez Gene 175359 Entrez gene
SwissProt Q9UA63 EMBL-EBI
WormBase CE20141 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC68918 Hypothetical protein W04B5.4 [Caenorhabditis elegans] ref|NP_497550.1| W04B5.4 [Caenorhabditis elegans] 1.0E-115
blast CAE69463 Hypothetical protein CBG15659 [Caenorhabditis briggsae] 1.0E-105
blast AAC68918 Hypothetical protein W04B5.4 [Caenorhabditis elegans] ref|NP_497550.1| W04B5.4 [Caenorhabditis elegans] 1.0E-103
blast CAE69463 Hypothetical protein CBG15659 [Caenorhabditis briggsae] 9.0E-94
blast AAC68918 Hypothetical protein W04B5.4 [Caenorhabditis elegans] ref|NP_497550.1| W04B5.4 [Caenorhabditis elegans] 8.0E-85
blast CAE69463 Hypothetical protein CBG15659 [Caenorhabditis briggsae] 5.0E-77
blast AAL49140 RE56957p [Drosophila melanogaster] 6.0E-9
Sequence
Target Sequence 
>C. ELEGANS:184167_S_AT
atcgctgggtataccgtacggacaacaaatggacccgctacttgccacgtagaaatgacg
ccgtcaaagagtttgactacgataagaatggagcatctctcaaacattatactgaagaaa
tttcagaggaaatgcgcccaaaacaagcgtcaaaactgtggatggcgtggctctaccgtg
acacgtctgccgagccgaaatggaccaaaaaacacgtggaaaacctgtttggagcggatt
ttaaggtcggtcgaatggaagtgttccgtaacactgagctcatcaacacagagctctgga
aaatcaaacatctcatcgaattgcgaccggttgagttcaaaaatggcgtcgagccgaccg
aagacgacattttttcgacgagcctggcaccaaatggacagtgtgaagtgattcagggtg
gacatgtggcatcggaaaatgatttgaaattggctgatgggaagcagtggacccgaaaag
agctgggacgtgacctcaacaagaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCGCTGGGTATACCGTACGGACAA 93 27 32 Antisense
ACCCGCTACTTGCCACGTAGAAATG 505 87 64 Antisense
GGAGCATCTCTCAAACATTATACTG 316 521 121 Antisense
AAAACTGTGGATGGCGTGGCTCTAC 452 131 183 Antisense
TGGAGCGGATTTTAAGGTCGGTCGA 375 547 261 Antisense
TGTTCCGTAACACTGAGCTCATCAA 203 563 293 Antisense
ACATCTCATCGAATTGCGACCGGTT 372 105 339 Antisense
GCGTCGAGCCGACCGAAGACGACAT 320 289 377 Antisense
TTTTTTCGACGAGCCTGGCACCAAA 528 673 401 Antisense
ATTCAGGGTGGACATGTGGCATCGG 666 7 442 Antisense
AGCTGGGACGTGACCTCAACAAGAA 232 81 512 Antisense
Sequence Source Affymetrix Proprietary Database