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[184164_at]

Description Y56A3A.3 /REP_DB=WormBase Gene ID /WP=CE22574 /TR=Q9U228 /GB=CAB60512.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 37      6grp_high1_10_10 > 73      4grp_highest_8_8 > 40      4grp_high_8_8 > 43      6grp_highest_nonorm > 36      6grp_total > 63      4grp_total > 53      6grp_highest_sub > 03_04      6grp_cgc > 76      6grp_high1_sub > 03_04      6grp_cgc_sub > 03_04      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 939
1 2 3 4 range
A 2556.2
(3)
P 		2422.4
(1.9)
P 		2102.3
(-0.7)
P 		1818.5
(-3)
P 		738
B 2474.1
(3)
P 		2463.2
(2.9)
P 		2106.3
(0)
P 		1729.2
(-3)
P 		745
C 2474.6
(3)
P 		2356.6
(2.2)
P 		1949.3
(-0.7)
P 		1617.1
(-3)
P 		858
avr 2501.6
(3) 		2414.1
(2.3) 		2052.6
(-0.5) 		1721.6
(-3) 		780

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2895
1 2 3 4 5 6 range
A 2549.8
(-3)
P 		3119.8
(-1.6)
P 		4750.2
(2.3)
P 		5038.3
(3)
P 		3313.5
(-1.2)
P 		3067.9
(-1.8)
P 		2489
B 2472.3
(-3)
P 		3062.1
(-1.8)
P 		5367.5
(3)
P 		4181.8
(0.5)
P 		3512.2
(-0.8)
P 		3232.1
(-1.4)
P 		2895
C 2493.9
(-3)
P 		2771.9
(-2.3)
P 		4360.6
(1.7)
P 		4857
(3)
P 		3461.3
(-0.5)
P 		3652.2
(-0.1)
P 		2363
avr 2505.3
(-3) 		2984.6
(-1.8) 		4826.1
(3) 		4692.4
(2.7) 		3429
(-0.6) 		3317.4
(-0.9) 		2321
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 665
1 2 3 4 range
A 930.7
(3)
P 		525.8
(-0.7)
P 		549.6
(-0.4)
P 		266.2
(-3)
P 		665
B 789
(3)
P 		608.3
(0.4)
P 		488.1
(-1.3)
P 		368.5
(-3)
P 		421
C 819.2
(3)
P 		677.5
(1.4)
P 		679.4
(1.5)
P 		272.7
(-3)
P 		547
avr 846.3
(3) 		603.9
(0.3) 		572.4
(-0) 		302.5
(-3) 		544

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 957
1 2 3 4 5 6 range
A 470
(-3)
P 		1109.6
(1.8)
P 		1034.5
(1.2)
P 		1277.8
(3)
P 		999.9
(0.9)
P 		746.9
(-0.9)
P 		808
B 860.2
(-3)
P 		1019.2
(-1.3)
P 		1303.4
(1.7)
P 		1107.6
(-0.4)
P 		1427
(3)
P 		1205.2
(0.7)
P 		567
C 924.4
(-1.7)
P 		845.8
(-3)
P 		1109.9
(1.2)
P 		976.7
(-0.9)
P 		1120
(1.4)
P 		1222.6
(3)
P 		377
avr 751.5
(-3) 		991.5
(0.3) 		1149.3
(2.5) 		1120.7
(2.1) 		1182.3
(3) 		1058.2
(1.3) 		431
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184164_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6281
Sequence Type Exemplar sequence
Representative Public ID Y56A3A.3 NCBI
Target Description Y56A3A.3 /REP_DB=WormBase Gene ID /WP=CE22574 /TR=Q9U228 /GB=CAB60512.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067135(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067135 NCBI Caenorhabditis elegans MIF (macrophage migration inhibitory factor) related family member (mif-1) (mif-1) mRNA, complete cds. 11/11 None
SNAP00000035188 ENSEMBL cdna:SNAP chromosome:CEL140:III:11848031:11849207:1 11/11 None
Y56A3A.3.1 ENSEMBL cdna:known chromosome:CEL140:III:11848026:11849386:1 gene:Y56A3A.3 11/11 None
Y56A3A.3.2 ENSEMBL cdna:known chromosome:CEL140:III:11848028:11849480:1 gene:Y56A3A.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000035237 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:11848026-11849203(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol mif-1
Ensembl Y56A3A.3
Entrez Gene 176615 Entrez gene
SwissProt Q9U228 EMBL-EBI
WormBase CE22574 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB60512 Hypothetical protein Y56A3A.3 [Caenorhabditis elegans] ref|NP_499536.1| MIF (macrophage migration inhibitory factor) related family member (mif-1) [Caenorhabditis elegans] 2.0E-61
blast CAE67727 Hypothetical protein CBG13302 [Caenorhabditis briggsae] 2.0E-43
blast AAL12629 macrophage migration inhibitory factor-like protein [Trichinella spiralis] 6.0E-21
Protein Domains
Database ID Description E-Value
Pfam IPR001398 EMBL-EBI Macrophage migration inhibitory factor 7.0E-31
Sequence
Target Sequence 
>C. ELEGANS:184164_AT
tgccagttttctccatcaatgtcaacgtaaaggtccctgccgagaagcagaatgagatct
tgaaagagttgtcaactgttctcggaaagcttctcaacaagccggagcagtacatgtgta
ttcacttccacgaggatcagggaatcctgtatgctgggaccactgagccagcaggctttg
ccgtgttgaaatcgatcggcggagttggaagcgctaagcagaataatgcgatttccgccg
tcgttttcccgattattgagaaacatcttgggattccaggaaatcgcctctacatcgaat
tcgtcaacctcggcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCCAGTTTTCTCCATCAATGTCAA 505 585 14 Antisense
AATGTCAACGTAAAGGTCCCTGCCG 436 159 31 Antisense
GTCAACTGTTCTCGGAAAGCTTCTC 118 465 84 Antisense
GTACATGTGTATTCACTTCCACGAG 627 459 123 Antisense
CCTGTATGCTGGGACCACTGAGCCA 571 265 159 Antisense
CACTGAGCCAGCAGGCTTTGCCGTG 550 183 174 Antisense
GGCTTTGCCGTGTTGAAATCGATCG 129 537 187 Antisense
AATAATGCGATTTCCGCCGTCGTTT 432 171 235 Antisense
GCCGTCGTTTTCCCGATTATTGAGA 174 283 250 Antisense
GATTCCAGGAAATCGCCTCTACATC 645 429 285 Antisense
TACATCGAATTCGTCAACCTCGGCG 505 643 304 Antisense
Sequence Source Affymetrix Proprietary Database