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[184162_at]

Description T14B4.6 /REP_DB=WormBase Gene ID /WP=CE04953 /GEN=dpy-2 /GB=AAK31557.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 10      6grp_high1_10_10 > 23      6grp_highest_nonorm > 21      6grp_total > 03      4grp_total > 49      6grp_cgc > 40      6grp_high1_sub > 02      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3789
1 2 3 4 range
A 39.2
(-3)
A 		3795.7
(3)
P 		2491.6
(0.9)
P 		67.8
(-3)
A 		3757
B 82.6
(-2.9)
A 		2699.3
(2.6)
P 		2913.3
(3)
P 		20.7
(-3)
A 		2893
C 26.5
(-3)
A 		2581.7
(3)
P 		2419.3
(2.6)
P 		7.2
(-3)
A 		2575
avr 49.4
(-3) 		3025.6
(3) 		2608.1
(2.2) 		31.9
(-3) 		2994

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1413
1 2 3 4 5 6 range
A 1816.8
(3)
P 		1562.9
(1.9)
P 		1086.8
(-0.2)
P 		1729.3
(2.6)
P 		792.2
(-1.5)
P 		439.3
(-3)
P 		1378
B 1564.4
(2.3)
P 		1711.5
(3)
P 		1113.9
(0.3)
P 		1347.8
(1.3)
P 		807.3
(-1.1)
P 		403.6
(-3)
P 		1308
C 1674.9
(2.7)
P 		1705.8
(2.9)
P 		1199.6
(0.5)
P 		1733.4
(3)
P 		997.3
(-0.4)
P 		435.4
(-3)
P 		1298
avr 1685.4
(3) 		1660.1
(2.9) 		1133.4
(0.4) 		1603.5
(2.6) 		865.6
(-0.9) 		426.1
(-3) 		1259
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 842
1 2 3 4 range
A 14.3
(-3)
A 		823.9
(3)
P 		651.4
(1.7)
P 		9.9
(-3)
A 		814
B 26.3
(-2.8)
A 		666.7
(2.9)
P 		675.1
(3)
P 		4.4
(-3)
A 		671
C 8.8
(-2.9)
A 		742.3
(2.3)
P 		843.2
(3)
P 		1.2
(-3)
A 		842
avr 16.5
(-2.9) 		744.3
(3) 		723.2
(2.8) 		5.2
(-3) 		739

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 514
1 2 3 4 5 6 range
A 334.9
(0)
P 		555.8
(3)
P 		236.7
(-1.3)
P 		438.6
(1.4)
P 		239
(-1.2)
P 		107
(-3)
P 		449
B 544.3
(2.6)
P 		569.7
(3)
P 		270.5
(-1.3)
P 		357
(-0)
P 		328
(-0.5)
P 		150.5
(-3)
P 		419
C 620.8
(3)
P 		520.5
(1.7)
P 		305.3
(-1)
P 		348.6
(-0.4)
P 		322.7
(-0.8)
P 		145.8
(-3)
P 		475
avr 500
(2.3) 		548.7
(3) 		270.8
(-1) 		381.4
(0.6) 		296.6
(-0.7) 		134.4
(-3) 		414
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184162_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5671
Sequence Type Exemplar sequence
Representative Public ID T14B4.6 NCBI
Target Description T14B4.6 /REP_DB=WormBase Gene ID /WP=CE04953 /GEN=dpy-2 /GB=AAK31557.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062966(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062966 NCBI Caenorhabditis elegans DumPY : shorter than wild-type family member (dpy-2) (dpy-2) mRNA, complete cds. 11/11 A 
SNAP00000000074 ENSEMBL cdna:SNAP chromosome:CEL140:II:6714234:6715732:1 11/11 None
GENEFINDER00000000086 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6714234:6716632:1 11/11 None
T14B4.6 ENSEMBL cdna:known chromosome:CEL140:II:6714232:6715915:1 gene:T14B4.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6714230-6715729(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol dpy-2
Ensembl T14B4.6
Entrez Gene 174107 Entrez gene
SwissProt P35799 EMBL-EBI
WormBase CE04953 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6817 phosphate transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5737 cytoplasm inferred from electronic annotation QuickGO AmiGO
5581 collagen inferred from sequence similarity QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
42302 structural constituent of cuticle inferred from electronic annotation QuickGO AmiGO
42329 structural constituent of cuticle (sensu Nematoda) inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_495367 DumPY : shorter than wild-type family member (dpy-2) [Caenorhabditis elegans] gb|AAK31557.1| Dumpy : shorter than wild-type protein 2 [Caenorhabditis elegans] 1.0E-143
blast NP_495367 DumPY : shorter than wild-type family member (dpy-2) [Caenorhabditis elegans] gb|AAK31557.1| Dumpy : shorter than wild-type protein 2 [Caenorhabditis elegans] 1.0E-129
blast P35799 Cuticle collagen dpy-2 precursor (Dumpy-2 protein) 1.0E-125
blast AAA17398 collagen [Caenorhabditis elegans] 1.0E-124
Protein Domains
Database ID Description E-Value
Pfam IPR002486 EMBL-EBI Nematode cuticle collagen, N-terminal 5.0E-6
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 1.6E-8
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 4.2E-12
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495367.1 1 26-48
T14B4.6 1 26-48
SNAP00000000074 1 26-48
GENEFINDER00000000086 1 26-48
Sequence
Target Sequence 
>C. ELEGANS:184162_AT
gattcccaggtgatccaggagagtatggaattggaggaagaccaggatccgatggaatgc
cagggaaaccaggagatccaggactcgcaggaccaattggaccaccaggagaatctggac
caattggagataagggaagaacaccagaagcacatgttattccaggaccacctggagatt
ctggattaccaggaccatggggaccaccaggatcagctggtatgcctggagaagatggat
acgcaggaacacctggagagaagggatggccaggaccgccaggagcaccaggaccaggtg
gaatgcctggaccaaatggtccaactggagaacaaggaccttctggtactcctggtactt
gtgtttgccaagatactgaagtagttatgaatgatgaaaagggacgtgttccagctcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTCCCAGGTGATCCAGGAGAGTA 427 433 560 Antisense
CAGGACTCGCAGGACCAATTGGACC 131 209 638 Antisense
GCACATGTTATTCCAGGACCACCTG 162 315 709 Antisense
GATTACCAGGACCATGGGGACCACC 39 431 743 Antisense
ACCATGGGGACCACCAGGATCAGCT 391 85 753 Antisense
AGGATCAGCTGGTATGCCTGGAGAA 102 57 768 Antisense
GAAGATGGATACGCAGGAACACCTG 608 335 790 Antisense
GGAGAGAAGGGATGGCCAGGACCGC 80 515 814 Antisense
GAACAAGGACCTTCTGGTACTCCTG 431 353 889 Antisense
GTACTCCTGGTACTTGTGTTTGCCA 575 459 905 Antisense
TGAAAAGGGACGTGTTCCAGCTCCA 241 571 954 Antisense
Sequence Source Affymetrix Proprietary Database