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[184155_at]

Description C45B11.4 /REP_DB=WormBase Gene ID /WP=CE05428 /TR=Q18640 /GB=CAA98432.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 72      4grp_total > 05      6grp_cgc > 75      6grp_cgc_sub > 03_04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 172
1 2 3 4 range
A 46
(-0.1)
A 		9.7
(-3)
A 		29.5
(-1.4)
A 		85.4
(3)
P 		76
B 29.3
(-0.3)
A 		13.3
(-2)
A 		3.2
(-3)
A 		61.1
(3)
A 		58
C 58.4
(-1.4)
A 		15.8
(-3)
A 		40
(-2.1)
A 		175.2
(3)
P 		159
avr 44.6
(-1) 		12.9
(-3) 		24.2
(-2.3) 		107.2
(3) 		94

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 62
1 2 3 4 5 6 range
A 31
(-1.3)
A 		15.4
(-3)
A 		69
(3)
A 		44.1
(0.2)
A 		58.3
(1.8)
A 		22.4
(-2.2)
A 		54
B 7.5
(-3)
A 		45.9
(1.4)
A 		45.4
(1.4)
A 		59.4
(3)
A 		54.1
(2.4)
A 		36.8
(0.4)
A 		52
C 10.1
(-2.9)
A 		30.4
(-0.6)
A 		8.8
(-3)
A 		63
(3)
A 		27.3
(-1)
A 		26
(-1.1)
A 		54
avr 16.2
(-3) 		30.6
(-0.8) 		41.1
(0.8) 		55.5
(3) 		46.6
(1.6) 		28.4
(-1.1) 		39
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 29
1 2 3 4 range
A 16.8
(3)
A 		2.1
(-3)
A 		7.7
(-0.7)
A 		12.5
(1.2)
P 		15
B 9.4
(1.2)
A 		3.3
(-1.7)
A 		0.7
(-3)
A 		13
(3)
A 		12
C 19.3
(0.6)
A 		4.5
(-3)
A 		13.9
(-0.7)
A 		29.5
(3)
P 		25
avr 15.2
(1.8) 		3.3
(-3) 		7.4
(-1.4) 		18.3
(3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 5.7
(-2.9)
A 		5.5
(-3)
A 		15
(1.7)
A 		11.2
(-0.2)
A 		17.6
(3)
A 		5.5
(-3)
A 		12
B 2.6
(-3)
A 		15.3
(0.9)
A 		11
(-0.4)
A 		15.7
(1.1)
A 		22
(3)
A 		13.7
(0.4)
A 		19
C 3.7
(-2.1)
A 		9.3
(1.1)
A 		2.2
(-3)
A 		12.7
(3)
A 		8.8
(0.8)
A 		8.7
(0.7)
A 		11
avr 4
(-3) 		10
(-0) 		9.4
(-0.3) 		13.2
(1.6) 		16.1
(3) 		9.3
(-0.4) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184155_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13087
Sequence Type Exemplar sequence
Representative Public ID C45B11.4 NCBI
Target Description C45B11.4 /REP_DB=WormBase Gene ID /WP=CE05428 /TR=Q18640 /GB=CAA98432.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073412(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073412 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-74) (srh-74) mRNA, complete cds. 11/11 None
GENEFINDER00000011926 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:11048338:11050691:-1 9/11 None
C45B11.4 ENSEMBL cdna:known chromosome:CEL140:V:11049815:11051332:-1 gene:C45B11.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11049814-11051332(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol srh-74
Ensembl C45B11.4
Entrez Gene 183468 Entrez gene
SwissProt Q18640 EMBL-EBI
WormBase CE05428 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA98432 Hypothetical protein C45B11.4 [Caenorhabditis elegans] ref|NP_505813.1| Serpentine Receptor, class H family member (srh-74) [Caenorhabditis elegans] 1.0E-180
blast CAA98431 Hypothetical protein C45B11.3 [Caenorhabditis elegans] ref|NP_505812.1| DeHydrogenases, Short chain family member (dhs-18) [Caenorhabditis elegans] 1.0E-155
blast CAE75163 Hypothetical protein CBG23100 [Caenorhabditis briggsae] 1.0E-141
blast CAE75160 Hypothetical protein CBG23097 [Caenorhabditis briggsae] 1.0E-121
blast CAE62784 Hypothetical protein CBG06955 [Caenorhabditis briggsae] 1.0E-118
blast CAB04680 Hypothetical protein T04C12.2a [Caenorhabditis elegans] ref|NP_001024099.1| Serpentine Receptor, class H family member (srh-75) [Caenorhabditis elegans] 1.0E-116
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 8.0E-110
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 6.2E-36
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505813.1 7 32-54,67-89,109-131,152-174,215-237,258-280,295-314
C45B11.4 7 32-54,67-89,109-131,152-174,215-237,258-280,295-314
GENEFINDER00000011926 5 7-29,39-59,80-102,141-163,184-206
Sequence
Target Sequence 
>C. ELEGANS:184155_AT
ccattgtgggatacctcataccgccctttttccacattccagatcaaaatgcagcaaaat
taaatctgcttcagacaataccatgccccacggaagagttcttttacagtgaaattttcg
ttttagcaaccgatgatttttggcacacttatctctggatgtttacaactattatagtta
ttggaatttttattcaagtcgctttcttttttctctgttgtttatattatatttattttt
ccacaacaatcactctttctccgaaaacaaaaaagtatcaacgtacatttttcttaggaa
caattgctcaagcactggttccattgattttccttttggcaccagcggcacttgtatttt
tatccattttctttaattactatgatcaatctcttaataattttattgttttatttatct
catttcatgattttgtctcaacgtttataattattctgattcaccatccatat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCATTGTGGGATACCTCATACCGCC 298 269 494 Antisense
ATCTGCTTCAGACAATACCATGCCC 126 33 557 Antisense
TACCATGCCCCACGGAAGAGTTCTT 127 651 572 Antisense
GATTTTTGGCACACTTATCTCTGGA 337 429 628 Antisense
TATTCAAGTCGCTTTCTTTTTTCTC 632 661 684 Antisense
AACAATCACTCTTTCTCCGAAAACA 641 133 738 Antisense
GTATCAACGTACATTTTTCTTAGGA 366 443 768 Antisense
AATTGCTCAAGCACTGGTTCCATTG 686 177 795 Antisense
CATTGATTTTCCTTTTGGCACCAGC 680 215 815 Antisense
CACCAGCGGCACTTGTATTTTTATC 702 201 833 Antisense
AATTATTCTGATTCACCATCCATAT 631 175 942 Antisense
Sequence Source Affymetrix Proprietary Database