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[184150_at]

Description F21F3.1 /REP_DB=WormBase Gene ID /WP=CE09531 /TR=P91268 /GB=AAB42278.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 63      6grp_high1_10_10 > 36      4grp_highest_8_8 > 46      4grp_high_8_8 > 11      6grp_highest_nonorm > 05      6grp_total > 35      4grp_total > 14      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9388
1 2 3 4 range
A 10516.3
(2.6)
P 		5502.6
(-2)
P 		4463.8
(-3)
P 		10943.7
(3)
P 		6480
B 9944.3
(0.5)
P 		6189.2
(-1.9)
P 		4560.4
(-3)
P 		13851.3
(3)
P 		9291
C 9754.1
(1)
P 		5787.1
(-2.4)
P 		5029.6
(-3)
P 		12066.8
(3)
P 		7037
avr 10071.6
(1.3) 		5826.3
(-2.1) 		4684.6
(-3) 		12287.3
(3) 		7603

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4878
1 2 3 4 5 6 range
A 6369.5
(1.8)
P 		5969.4
(0.9)
P 		6957.7
(3)
P 		4109.4
(-3)
P 		4648.1
(-1.9)
P 		5611.3
(0.2)
P 		2848
B 8977.3
(3)
P 		7536
(1)
P 		7380.4
(0.7)
P 		4896.2
(-2.8)
P 		4737.1
(-3)
P 		6553.6
(-0.4)
P 		4240
C 6644.4
(1.9)
P 		7188.3
(3)
P 		5458.7
(-0.4)
P 		5715.5
(0.1)
P 		4099.5
(-3)
P 		7074.5
(2.8)
P 		3089
avr 7330.4
(3) 		6897.9
(2.1) 		6598.9
(1.5) 		4907
(-2.1) 		4494.9
(-3) 		6413.1
(1.1) 		2836
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2772
1 2 3 4 range
A 3829
(3)
P 		1194.5
(-2.9)
P 		1166.9
(-3)
P 		1601.9
(-2)
P 		2662
B 3171.1
(3)
P 		1528.6
(-1.7)
P 		1056.7
(-3)
P 		2951.7
(2.4)
P 		2114
C 3228.9
(3)
P 		1663.8
(-3)
P 		1753
(-2.7)
P 		2034.7
(-1.6)
P 		1565
avr 3409.7
(3) 		1462.3
(-2.6) 		1325.5
(-3) 		2196.1
(-0.5) 		2084

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2082
1 2 3 4 5 6 range
A 1174.2
(-2.3)
P 		2123
(3)
P 		1515.2
(-0.4)
P 		1042.2
(-3)
P 		1402.6
(-1)
P 		1366.2
(-1.2)
P 		1081
B 3123.7
(3)
P 		2508.4
(1)
P 		1792.2
(-1.4)
P 		1296.8
(-3)
P 		1924.6
(-0.9)
P 		2443.8
(0.8)
P 		1827
C 2462.8
(3)
P 		2193.4
(1.8)
P 		1389.3
(-1.9)
P 		1149.4
(-3)
P 		1326.5
(-2.2)
P 		2368.3
(2.6)
P 		1313
avr 2253.6
(2.9) 		2274.9
(3) 		1565.6
(-0.8) 		1162.8
(-3) 		1551.2
(-0.9) 		2059.4
(1.8) 		1112
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184150_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2413
Sequence Type Exemplar sequence
Representative Public ID F21F3.1 NCBI
Target Description F21F3.1 /REP_DB=WormBase Gene ID /WP=CE09531 /TR=P91268 /GB=AAB42278.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059074(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059074 NCBI Caenorhabditis elegans F21F3.1 (F21F3.1) mRNA, complete cds. 11/11 None
GENEFINDER00000035685 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:4906848:4911760:1 11/11 None
SNAP00000035675 ENSEMBL cdna:SNAP chromosome:CEL140:I:4909366:4911760:1 11/11 None
F21F3.1 ENSEMBL cdna:known chromosome:CEL140:I:4906827:4911824:1 gene:F21F3.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4906298-4911760(+) 97.78 100.0
Public Domain and Genome References
Gene Title
Ensembl F21F3.1
Entrez Gene 172108 Entrez gene
SwissProt P91268 EMBL-EBI
WormBase CE32870 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1632701_AT Peptidyl-hydroxyglycine-&agr;-amidating lyase dm
DROSGENOME1:147795_AT Peptidyl-hydroxyglycine-&agr;-amidating lyase dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6518 peptide metabolism inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4504 peptidylglycine monooxygenase activity inferred from electronic annotation QuickGO AmiGO
5507 copper ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB42278 Hypothetical protein F21F3.1 [Caenorhabditis elegans] ref|NP_491475.2| F21F3.1 [Caenorhabditis elegans] 0.0
blast AAB42278 Hypothetical protein F21F3.1 [Caenorhabditis elegans] ref|NP_491475.2| F21F3.1 [Caenorhabditis elegans] 1.0E-172
blast CAE73111 Hypothetical protein CBG20492 [Caenorhabditis briggsae] 1.0E-168
blast CAE73111 Hypothetical protein CBG20492 [Caenorhabditis briggsae] 1.0E-155
blast P12890 Peptidyl-glycine alpha-amidating monooxygenase II precursor (Peptide C-terminal alpha-amidating enzyme II) (AE-II) gb|AAA49667.1| alpha-amidating enzyme precursor 1.0E-50
Protein Domains
Database ID Description E-Value
Pfam IPR001258 EMBL-EBI NHL repeat 47.0
Pfam IPR001258 EMBL-EBI NHL repeat 8.9E-10
Pfam IPR001258 EMBL-EBI NHL repeat 4.2E-10
Pfam IPR001258 EMBL-EBI NHL repeat 1.5E-6
Pfam IPR001258 EMBL-EBI NHL repeat 47.0
Pfam IPR001258 EMBL-EBI NHL repeat 8.9E-10
Pfam IPR001258 EMBL-EBI NHL repeat 4.2E-10
Pfam IPR001258 EMBL-EBI NHL repeat 1.5E-6
Sequence
Target Sequence 
>C. ELEGANS:184150_AT
ggatcaagctcatttctgcaagccaactgatgtggcagtggctaagaatggtcatatctt
tgtggccgatgggtactgtaacagccgtattctcaagtttgacgccaagggaaatctgat
ggctcagatcaatgcagctactgaagaaaaccaaccatctgaattcgtcgttccacactc
tttgtctctcattgaggatatgaatattgtctgcgttgctgacagagaaaaccagcgtgt
tcaatgcttctcagctggtctttcggagggagatcgtacacttccaacaggaattccaat
aacttcggccaccgacattggccgtgtgttcgcaattcgagagcgtgagcactatctgat
tggtgtgacgggtaacagtgaagacgttgaagcacaaatgttctcaattgatatgcaaac
tggaaagactgagacattcgcaaaaggagttcgtaatactcatgcacttgcaattgctgc
cgacggagtcatgttcgtgtcac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATCAAGCTCATTTCTGCAAGCCA 531 511 684 Antisense
GAATGGTCATATCTTTGTGGCCGAT 295 333 729 Antisense
GTACTGTAACAGCCGTATTCTCAAG 264 459 756 Antisense
CCAACCATCTGAATTCGTCGTTCCA 175 277 834 Antisense
CACACTCTTTGTCTCTCATTGAGGA 570 195 857 Antisense
TTCAATGCTTCTCAGCTGGTCTTTC 493 685 923 Antisense
GAGGGAGATCGTACACTTCCAACAG 16 403 949 Antisense
AATAACTTCGGCCACCGACATTGGC 640 171 981 Antisense
GACATTGGCCGTGTGTTCGCAATTC 451 371 997 Antisense
GTTCGTAATACTCATGCACTTGCAA 507 441 1132 Antisense
GCCGACGGAGTCATGTTCGTGTCAC 593 283 1162 Antisense
Sequence Source Affymetrix Proprietary Database