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[184145_at]

Description F30H5.2 /REP_DB=WormBase Gene ID /WP=CE01926 /TR=Q09982 /GB=AAA68406.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 07      4grp_total > 44      6grp_cgc > 12      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 37
1 2 3 4 range
A 43.7
(3)
A 		19.8
(-0.9)
A 		8.2
(-2.8)
A 		7.2
(-3)
A 		37
B 27.9
(1.4)
A 		35.2
(3)
A 		23.8
(0.5)
A 		8.1
(-3)
A 		27
C 9.4
(-2.9)
A 		31.5
(1.9)
A 		8.8
(-3)
A 		36.5
(3)
A 		28
avr 27
(2.3) 		28.8
(3) 		13.6
(-3) 		17.3
(-1.5) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 187
1 2 3 4 5 6 range
A 132.1
(3)
A 		64.9
(-1.1)
M 		43.8
(-2.3)
A 		87.3
(0.3)
A 		60.8
(-1.3)
A 		32.7
(-3)
A 		99
B 189
(3)
P 		92.3
(-0.5)
P 		36.8
(-2.6)
A 		44.1
(-2.3)
A 		28.2
(-2.9)
A 		25.5
(-3)
A 		164
C 194.5
(3)
P 		96
(-0.2)
A 		11.8
(-2.9)
A 		62.8
(-1.2)
A 		36.2
(-2.1)
A 		7.3
(-3)
A 		187
avr 171.9
(3) 		84.4
(-0.5) 		30.8
(-2.6) 		64.7
(-1.3) 		41.7
(-2.2) 		21.8
(-3) 		150
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 15.9
(3)
A 		4.3
(-1.7)
A 		2.1
(-2.6)
A 		1
(-3)
A 		15
B 8.9
(3)
A 		8.7
(2.8)
A 		5.5
(0.2)
A 		1.7
(-3)
A 		7
C 3.1
(-3)
A 		9
(3)
A 		3.1
(-3)
A 		6.2
(0.2)
A 		6
avr 9.3
(3) 		7.3
(1.1) 		3.6
(-2.4) 		3
(-3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 70
1 2 3 4 5 6 range
A 24.4
(3)
A 		23.1
(2.5)
M 		9.5
(-2.5)
A 		22.1
(2.2)
A 		18.4
(0.8)
A 		8
(-3)
A 		16
B 65.8
(3)
P 		30.7
(-0.7)
P 		8.9
(-3)
A 		11.7
(-2.7)
A 		11.5
(-2.7)
A 		9.5
(-2.9)
A 		57
C 72.1
(3)
P 		29.3
(-0.7)
A 		3
(-2.9)
A 		12.6
(-2.1)
A 		11.7
(-2.2)
A 		2.4
(-3)
A 		70
avr 54.1
(3) 		27.7
(-0.3) 		7.1
(-2.9) 		15.5
(-1.9) 		13.9
(-2.1) 		6.6
(-3) 		48
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 184145_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8257
Sequence Type Exemplar sequence
Representative Public ID F30H5.2 NCBI
Target Description F30H5.2 /REP_DB=WormBase Gene ID /WP=CE01926 /TR=Q09982 /GB=AAA68406.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064803(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064803 NCBI Caenorhabditis elegans Neuropeptide-Like Protein family member (nlp-32) (nlp-32) mRNA, complete cds. 11/11 None
SNAP00000018209 ENSEMBL cdna:SNAP chromosome:CEL140:III:486167:486531:-1 11/11 None
GENEFINDER00000018217 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:486167:486531:-1 11/11 None
F30H5.2 ENSEMBL cdna:known chromosome:CEL140:III:486167:486531:-1 gene:F30H5.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:486166-486531(-) 63.52 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nlp-32
Ensembl F30H5.2
Entrez Gene 185132 Entrez gene
SwissProt Q09982 EMBL-EBI
WormBase CE01926 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_497204 Neuropeptide-Like Protein family member (nlp-32) [Caenorhabditis elegans] gb|AAA68406.1| Neuropeptide-like protein protein 32 [Caenorhabditis elegans] sp|Q09982|NLP32_CAEEL Putative neuropeptide-like protein 32 precursor [Contains: YGGWG-amide; GGW-amide; GG-amide; GYG-amide; GGGWG-amide; GGGW-amide; GGG-amide; GFYGG-amide; GW-amide] 2.0E-23
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497204.1 1 7-29
F30H5.2 1 7-29
SNAP00000018209 1 7-29
GENEFINDER00000018217 1 7-29
Sequence
Target Sequence 
>C. ELEGANS:184145_AT
atgagacaattcaacttgctacttgtattctgtcttatcgcattgaccgccttgccggtt
ttctcttttccaaatgggctaactatggactccattgatatggagccaatgggagcattt
gatgaaaatggagccgctgatgaatcaccccgagtgaagcgttacggtggatggggaggt
cgtggtggatggggtcgcggaggcggccgtggatatggtggacgtggcggtggatgggga
ggacgtggaggtggctggggacgtggcggaggtggacgtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGAGACAATTCAACTTGCTACTTG 695 45 13 Antisense
TGCTACTTGTATTCTGTCTTATCGC 172 581 29 Antisense
TATTCTGTCTTATCGCATTGACCGC 588 663 38 Antisense
TTTCTCTTTTCCAAATGGGCTAACT 619 671 72 Antisense
ATGGGCTAACTATGGACTCCATTGA 439 49 86 Antisense
GATATGGAGCCAATGGGAGCATTTG 415 425 109 Antisense
AATGGAGCCGCTGATGAATCACCCC 204 161 139 Antisense
GAATCACCCCGAGTGAAGCGTTACG 180 325 154 Antisense
GAAGCGTTACGGTGGATGGGGAGGT 9 153 168 Antisense
GGACGTGGAGGTGGCTGGGGACGTG 260 523 253 Antisense
TGGGGACGTGGCGGAGGTGGACGTG 123 553 268 Antisense
Sequence Source Affymetrix Proprietary Database